Vasoactive intestinal peptide increases tyrosine hydroxylase activity in normal and neoplastic rat chromaffin cell cultures

Tischler, Arthur S.; Perlman, Robert L.; Costopoulos, Donna; Horwitz, Joel

doi:10.1016/0304-3940(85)90415-x

Cited by 33 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In further support of a wide distribution of this peptidergic regulation of TH throughout the sympathetic nervous system, secretin ( I p M ) and VIP (10 p M ) have also been found to stimulate TH activity in the upper six thoracic paravertebral ganglia of the rat, 1.8-and 3.4-fold, respectively [ p < 0.05 in each case by the Student's t test; (H. Chiou and R. Zigmond, unpublished observations)]. Furthermore, VIP has been found to increase TH activity in chromaffin cells of rat and bovine adrenal glands (Tischler et al, 1985;Craviso et al, 1987;Houchi et al, 1987), although secretin, interestingly, had no effect in bovine chromaffin cells.…”

Section: Peptidergic Stimulation Of Th Activity In the Sympathetic Nementioning

confidence: 93%

Effects of Peptides of the Secretin‐Glucagon Family and Cyclic Nucleotides on Tyrosine Hydroxylase Activity in Sympathetic Nerve Endings

Schwarzschild

Zigmond

1991

Journal of Neurochemistry

View full text Add to dashboard Cite

Previous studies have shown that certain peptides of the secretin-glucagon family stimulate tyrosine hydroxylase activity in sympathetic neurons of the superior cervical ganglion and three of its end organs, i.e., the iris, pineal gland, and submaxillary gland. To determine whether a similar regulation occurs in other sympathetic neurons, the effects of two of these peptides, secretin and vasoactive intestinal peptide, were examined in the right cardiac ventricle of the rat, a tissue innervated primarily by the middle and inferior cervical ganglia. Both peptides stimulated tyrosine hydroxylase activity, measured in situ, in this tissue. In addition, several second messenger systems were investigated as possible mediators of this peptidergic stimulation of tyrosine hydroxylase activity in autonomic end organs. 8-Bromoadenosine 3',5'-cyclic monophosphate and forskolin elevated tyrosine hydroxylase activity in slices of both the right ventricle and the submaxillary gland. 8-Bromoguanosine 3',5'-cyclic monophosphate also stimulated tyrosine hydroxylase activity in both tissues, whereas nitroprusside stimulated activity only in the submaxillary slices. Furthermore, the phosphodiesterase inhibitors 3-isobutyl-1-methylxanthine and/or Ro 20-1724 potentiated the stimulation by secretin, as well as the stimulations by forskolin and nitroprusside. Phorbol 12,13-dibutyrate also stimulated tyrosine hydroxylase activity in cardiac and submaxillary slices; however, no potentiation of these effects was seen following addition of either phosphodiesterase inhibitor. These data, taken together with those of previous studies, suggest a role for a cyclic nucleotide, probably adenosine 3',5'-cyclic monophosphate, in the peptidergic stimulation of tyrosine hydroxylase activity in sympathetic nerve terminals.

show abstract

Section: Peptidergic Stimulation Of Th Activity In the Sympathetic Nementioning

confidence: 93%

Effects of Peptides of the Secretin‐Glucagon Family and Cyclic Nucleotides on Tyrosine Hydroxylase Activity in Sympathetic Nerve Endings

Schwarzschild

Zigmond

1991

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…In the PNS, the differentiation and secretory profile of mammalian cells of the sympathoadrenal lineage have been shown to be modulated by VIP . For many cell types, however, the concentrations of VIP necessary for these responses are uncharacteristically high for specific peptide actions (Ip et al, 1982(Ip et al, , 1985Tischler et al, 1985 ;Audigier et al, 1986;Houchi et al, 1987;Malhotra et al, 1988Malhotra et al, , 1989Pincus et al, 1989Pincus et al, , 1990 . Micromolar levels of VIP, for example, are necessary to increase neuroblast mitosis, survival, neurite outgrowth, and cyclic AMP production in superior cervical ganglion (SCG) cultures from embryonic rats (Pincus et al, 1989(Pincus et al, , 1990 .…”

mentioning

confidence: 99%

“…Micromolar levels of VIP, for example, are necessary to increase neuroblast mitosis, survival, neurite outgrowth, and cyclic AMP production in superior cervical ganglion (SCG) cultures from embryonic rats (Pincus et al, 1989(Pincus et al, , 1990 . High concentrations of VIP are necessary to stimulate L-tyrosine, tetrahydrobiopterin:oxygen oxidoreductase (EC 1.14.16.2; tyrosine hydroxylase) activity, cyclic AMP production, and phospholipid breakdown in adult SCG cultures (Ip et al, 1982(Ip et al, , 1985Audigier et al, 1986) ; similarly high levels of VIP are required to augment second messenger production, calcium uptake, and catecholamine secretion and production in primary and neoplastic adrenal medullary chromaffin cell cultures (Tischler et al, 1985 ;Houchi et al ., 1987;Malhotra et al, 1988Malhotra et al, , 1989 . In some cases, VIP may be simulating the activities of other structurally related physiological ligands at sympathoadrenal cell receptors, and the PACAPs may be among the regulators of sympathetic and adrenal function.…”

mentioning

confidence: 99%

Pituitary Adenylate Cyclase‐Activating Polypeptide (PACAP) Regulation of Sympathetic Neuron Neuropeptide Y and Catecholamine Expression

May

Braas

1995

Journal of Neurochemistry

View full text Add to dashboard Cite

Two forms of pituitary adenylate cyclase-activating polypeptide (PACAP), the 38-and 27-amino-acid forms (PACAP38 and PACAP27, respectively), which share amino acid sequence homology with vasoactive intestinal peptide (VIP), were evaluated for their abilities to regulate sympathetic neuron catecholamine and neuropeptide Y (NPY) expression . PACAP38 and PACAP27 potently and efficaciously stimulated NPY and catecholamine secretion in primary cultured superior cervical ganglion (SCG) neurons; 100-to 1,000-fold higher concentrations of VIP were required to modulate secretion, suggesting that SCG neurons express the PACAP-selective type I receptor . PACAP38 elicited a sustained seven-to ninefold increase in the rate of NPY secretion and threefold stimulation in the rate of catecholamine release. PACAP38 and PACAP27 produced parallel neuronal NPY and catecholamine release, but cellular levels of NPY and catecholamines were differentially regulated. Sympathetic neuron NPY content was decreased, whereas cellular total catecholamine levels were elevated by the PACAP peptides ; total NPY and catecholamine levels (secreted plus cellular content) were increased. In concert with the increased total peptide and transmitter production, pro-NPY and tyrosine hydroxylase mRNA levels were elevated . Furthermore, PACAP38 was more efficacious than PACAP27 in regulating pro-NPY and tyrosine hydroxylase mRNA . SCG neuronal expression of mRNA encoding the type I PACAP receptor further supported the studies demonstrating that sympathetic neuronal levels of NPY and catecholamine content and secretion and mRNA are differentially regulated by the PACAP peptides . Key Words: Pituitary adenylate cyclase-activating polypeptide-Vasoactive intestinal peptide-Superior cervical ganglion-Cell culture-Neuropeptide-Tyrosine hydroxylase. J. Neurochem. 65,978-987 (1995) .Pituitary adenylate cyclase-activating polypeptides (PACAPs) belong to the vasoactive intestinal peptide (VIP)/ secretin/glucagon family of bioactive peptides and mediate various physiological functions in neuroendocrine tissues . Isolated originally from ovine hypo-

show abstract

“…In this regulatory pathway, VIP may act as a potent stimulatory factor, by innervating the adrenal cortex [4]. VIP acutely increases TH activity in cultures of dispersed normal adult rat chromaffin cells and of a PC 12 rat phaeochromocytoma cell line [29]. In our study it was also apparent that VIP and TH often occurred in the same nerve trunk and were also often co-localized in the same fibres.…”

Section: Discussionmentioning

confidence: 75%

Innervation of human adrenal gland and adrenal cortical lesions

Johansson

Grimelius

1999

Virchows Archiv

View full text Add to dashboard Cite

The innervation of the human adrenal gland and of cortical lesions was studied in sections of cortical tissue (n=10), hyperplastic cortical tissue (n=3), and tissue from cortical adenomas (n=5) and carcinomas (n=6). The presence and distribution of nerve structures containing neuronal markers indicating sympathetic and parasympathetic innervation were studied by immunohistochemistry and the co-existence and co-localization patterns of the different markers by immunofluorescence. The cortex and hyperplastic cortical tissue had a moderate to rich supply of nerve structures containing the typical neuronal markers: protein gene product 9.5 (PGP 9.5), neuron-specific enolase (NSE), small vesicle synaptic protein type 2 (SV2), and nerves showing immunoreactivity to the adrenergic marker tyrosine hydroxylase (TH). All these immunoreactive nerves were located predominantly adjacent to blood vessels, but also among parenchymal cells. The cortex showed numerous nerve structures containing the neuropeptide substance P (SP), neuropeptide Y (NPY) and vasoactive intestinal protein (VIP), but few nerves containing these peptides were seen in hyperplastic cortical tissue. Typical markers were occasionally observed in cortical adenomas but were not found in carcinomas, except in a few cases where PGP 9.5 and NSE were present, but only adjacent to necrotic areas. Nerves containing NPY and VIP occurred in varying numbers in both adenomas and carcinomas. NPY-and VIP-immunoreactive nerve structures were seen mostly alongside blood vessels. There were several types of co-existence. For instance, NSE/VIP-, TH/VIP-and TH/NPY-immunoreactive nerve structures were often seen in the same trunk, but were only partly co-localized.Abstract Adrenal cortex · Adrenal tumour · Innervation · Immunohistochemistry · Neuronal markers

show abstract

Vasoactive intestinal peptide increases tyrosine hydroxylase activity in normal and neoplastic rat chromaffin cell cultures

Cited by 33 publications

References 17 publications

Effects of Peptides of the Secretin‐Glucagon Family and Cyclic Nucleotides on Tyrosine Hydroxylase Activity in Sympathetic Nerve Endings

Effects of Peptides of the Secretin‐Glucagon Family and Cyclic Nucleotides on Tyrosine Hydroxylase Activity in Sympathetic Nerve Endings

Pituitary Adenylate Cyclase‐Activating Polypeptide (PACAP) Regulation of Sympathetic Neuron Neuropeptide Y and Catecholamine Expression

Innervation of human adrenal gland and adrenal cortical lesions

Contact Info

Product

Resources

About