Vasoactive Actions of Local Anaesthetics on Human Isolated Umbilical Veins and Arteries

Monuszko, E.; Halevy, S; Freese, K. J.; Liu-Barnett, M.; Altura, Burton M.; M.D., F.H.R.S. Dwight Reynolds

doi:10.1097/00132582-199001000-00045

Cited by 2 publications

(2 citation statements)

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“…The buffer was then switched to a modified Krebs buffer containing 90 mM KCl (24,29) for 2 min to generate smooth muscle contraction while the cardiomyocytes were paralyzed despite continuing electrical pacing at 0.1 Hz. The buffer was then switched to a modified Krebs buffer containing 90 mM KCl (24,29) for 2 min to generate smooth muscle contraction while the cardiomyocytes were paralyzed despite continuing electrical pacing at 0.1 Hz.…”

Section: Methodsmentioning

confidence: 99%

Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein

Liu

Feng

Jin

2014

American Journal of Physiology-Cell Physiology

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We recently demonstrated the abundant presence of cardiomyocytes in the wall of thoracic veins of adult mouse and rat. The highly differentiated morphology and myofilament protein contents of the venous cardiomyocytes suggested contractile functions. Here we further investigated the contractility of mouse and rat azygos venous rings compared with that of atrial strips and ventricular papillary muscle. 5-Bromo-4-chloro-indolyl-galactopyranoside (X-gal) staining of transgenic mouse vessels expressing lacZ under a cloned cardiac troponin T promoter demonstrated that the venous cardiomyocytes are discontinuous from atrial myocardium and aligned in the wall of thoracic veins perpendicular to the vessel axis. Histological sections displayed sarcomeric striations in the venous cardiomyocytes, which indicate an encirclement orientation of myofibrils in the vessel wall. Mechanical studies found that the rings of mouse and rat azygos vein produce strong cardiac type twitch contractions when stimulated with electrical pacing in contrast to the weak and slow smooth muscle contractions induced using 90 mM KCl. The twitch contraction and relaxation of mouse azygos veins further exhibited a cardiac type of β-adrenergic responses. Quantitative comparison showed that the contractions of venous cardiomyocytes are slightly slower than those of atrium muscle but significantly faster than those of ventricular papillary muscle. These novel findings indicate that the cardiomyocytes abundant in the wall of rodent thoracic veins possess fully differentiated cardiac muscle phenotype despite their anatomical and functional segregations from the heart.

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Section: Methodsmentioning

confidence: 99%

Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein

Liu

Feng

Jin

2014

American Journal of Physiology-Cell Physiology

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“…Strips were aerated with a gas mixture of 95% O 2 and 5% CO 2 throughout the experiment. This gas mixture was studied for in vitro organ bath experiments and provided similar CO 2 pressure and pH value to human plasma . Strips were initially placed under a resting tension of 2 g and were allowed to equilibrate for 1 h. During this period, the bath solution was changed every 15 min and the resting tension was readjusted to the 2 g level.…”

Section: Methodsmentioning

confidence: 99%

Effects of propofol and sevoflurane on isolated human umbilical arteries pre‐contracted with dopamine, adrenaline and noradrenaline

Gunduz

Arun

Bagci

et al. 2014

J of Obstet and Gynaecol

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Dopamine, adrenaline and noradrenaline elicit contractions in human umbilical arteries, and noradrenaline causes the highest contraction. Both propofol and sevoflurane inhibit these contractions in a dose-dependent manner. Propofol caused greater relaxation in the contractions elicited by dopamine and adrenaline while sevoflurane caused greater relaxation in the contraction elicited by noradrenaline.

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Vasoactive Actions of Local Anaesthetics on Human Isolated Umbilical Veins and Arteries

Cited by 2 publications

References 0 publications

Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein

Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein

Effects of propofol and sevoflurane on isolated human umbilical arteries pre‐contracted with dopamine, adrenaline and noradrenaline

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