Vascular graft healing: I. FTIR analysis of an implant model for studying the healing of a vascular graft

Lyman, Donald J.; Murray-Wijelath, Jacqueline

doi:10.1002/(sici)1097-4636(1999)48:2<172::aid-jbm12>3.0.co;2-x

Cited by 18 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13,14 Of interest is the increase in the 1034 cm Ϫ1 absorption relative to the 1076 cm Ϫ1 absorption, which would suggest the beginning of collagen IV formation in this mixture of saccharide-containing material. 5,10,11 Because collagen IV is an indicator of the presence of endothelial cells, 25 one would expect the detection (by histology techniques) of some endothelial cells on the luminal surface of the grafts. However, this was not the case.…”

Section: Discussionmentioning

confidence: 99%

“…There are also increases in the bands associated with collagen IV (1031 cm Ϫ1 band relative to the 1077 cm Ϫ1 band), 5,10,11 suggesting that some of these cellular membranes are from endothelial cells, because collagen IV has been reported to be a marker for endothelial cells. 25 Histologies did show that endothelial cells are present at this time point.…”

Section: Discussionmentioning

confidence: 99%

“…5,10,11 Because collagen IV is synthesized by endothelial cells and, as such, is an indicator of the presence of endothelial cells, 25 the lipid membranes noted above may be from endothelial cells. This IR observation is supported by the positive silver nitrate and Factor VIII/von Willebrand Factor (vWF) staining results on the 14-day explant sample.…”

Section: -Day Exposure To Flowing Bloodmentioning

confidence: 99%

See 2 more Smart Citations

Vascular graft healing. II. FTIR analysis of polyester graft samples from implanted bi-grafts

Lyman¹,

Murray-Wijelath²,

Ambrad–Chalela³

et al. 2001

J. Biomed. Mater. Res.

Self Cite

View full text Add to dashboard Cite

FTIR-ATR analysis has shown that the 4-step process for preclotting polyester vascular grafts results in a uniform and reproducible fibrin coating of the polyester fibers. Western blot analyses have shown that FN and VEGF are also present in this fibrin coating. FTIR-ATR analyses of explanted grafts indicate that, while the in vivo healing of these preclotted polyester grafts proceed through the inflammation, proliferation, and remodeling phases of normal wound healing, these phases are modified. Because the fibrin coating provides a nonporous barrier between peri-graft tissue and the flowing blood, these molecular changes are controlled by the interactions of blood-borne constituents with the lumenal surface of the preclotted graft. Also, a well prepared preclotted polyester graft shows a minimal inflammatory response. After implantation, the fibrin preclot is more than 90% gone by the fifth day. However, the proliferation phase, involving synthesis of new protein and polysaccharide materials to replace the fibrin, appears to have begun by the third day. Detection of collagen I in the 5-day explants suggests that the overlapping remodeling phase of healing has begun. Protein and saccharide materials continue to be synthesized and remodeled, and, by the tenth day, collagen IV is detected. By 14-days post-implantation, there is an increase in collagen IV and cellular membrane lipids. Because collagen IV is an indicator of the presence of endothelial cells, some of these cellular membranes must be of endothelial origin. Thus, it appears that FTIR-ATR can be a useful tool in the study of vascular healing.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: -Day Exposure To Flowing Bloodmentioning

confidence: 99%

See 1 more Smart Citation

Vascular graft healing. II. FTIR analysis of polyester graft samples from implanted bi-grafts

Lyman¹,

Murray-Wijelath²,

Ambrad–Chalela³

et al. 2001

J. Biomed. Mater. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Samples of ePTFE segments, retrieved at 1 and 3 h; 3, 5, 7, 10, and 14 days; and 4 and 8 weeks postimplantation, were analyzed by FTIR‐ATR. Empirical rules coupled with a number of experimental and theoretical studies7, 9–24 were used to assist in IR band assignments for these spectra.…”

Section: Resultsmentioning

confidence: 99%

“…A small band at 2120 cm −1 , due to a combination of water vibrations,28, 29 can be used for the subtraction of water, because biochemical materials usually do not absorb at this wavelength. In addition, the development of ATR techniques for examination of surfaces has allowed the nondestructive analysis of human tissue 7, 9–12, 16–21. These studies have shown that one can determine the presence of various collagens (and other proteins), cholesterol, and cholesterol esters in human tissue.…”

Section: Discussionmentioning

confidence: 99%

Vascular graft healing. III. FTIR analysis of ePTFE graft samples from implanted bigrafts

2004

Self Cite

View full text Add to dashboard Cite

A bigraft, composed of a 30-microm internodal distance expanded polytetrafluoroethylene (ePTFE) arterial graft segment and a preclotted polyethylene terephthalate arterial graft segment, was used to study the healing process of two different materials in the same dog. Healing was followed by FTIR-ATR spectroscopy and correlated with ELISA analyses of selected growth factors and matrix proteins. The FTIR analyses of the ePTFE explants from 1 and 3 h; 3, 5, 7, 10, and 14 days; and 4 and 8 weeks showed that the ePTFE grafts did not heal (endothelialize), but appeared to remain in overlapping inflammation and proliferation stages of wound healing. Although FN was found in the fibrin layer coating the luminal surface of the ePTFE, VEGF was not present. The inability of VEGF to complex with FN may be the result of FN binding sites being blocked or hidden by the conformation of the fibrin clot that forms on ePTFE. Also, TGF-beta was not present beyond the initial clot formation at 3 h postimplantation. The absence of these two growth factors may be an important factor in the lack of healing of 30-microm ePTFE grafts, because both were found to be present during the early stages of healing for the preclotted polyester grafts that did endothelialize.

show abstract

Role of fluid dynamics on the healing of an in vivo tissue engineered vascular graft

et al. 2005

Self Cite

View full text Add to dashboard Cite

A polyester (PET) reinforced fibrin-FN-VEGF-TGFbeta vascular graft, formed by a four-step preclotting technique of a porous PET arterial graft, shows the overlapping inflammation, proliferation, and remodeling steps of normal wound healing when implanted in the descending thoracic aorta (DTA) position in the dog, forming a surface layer of endothelial cells. While the DTA grafts readily healed (i.e., endothelialized), similar grafts implanted in the carotid-femoral artery position did not fully heal. Since the initial phases of healing were shown to be dependent upon the transport of blood-borne constituents to the graft surface, the extent of healing appears to be dependent on the fluid dynamics present in the artery-graft-artery construct. The length of the noncompliant graft, the construction of the anastomoses, bends in the construct, graft diameter, and graft compliance can affect the fluid dynamics in the implant, and thus the healing of the graft. This has clinical relevance for the testing and development of new vascular graft materials.

show abstract

Vascular graft healing: I. FTIR analysis of an implant model for studying the healing of a vascular graft

Cited by 18 publications

References 23 publications

Vascular graft healing. II. FTIR analysis of polyester graft samples from implanted bi-grafts

Vascular graft healing. II. FTIR analysis of polyester graft samples from implanted bi-grafts

Vascular graft healing. III. FTIR analysis of ePTFE graft samples from implanted bigrafts

Role of fluid dynamics on the healing of an in vivo tissue engineered vascular graft

Contact Info

Product

Resources

About