Role of fluid dynamics on the healing of an <i>in vivo</i> tissue engineered vascular graft

Lyman, Donald J.; Stewart, Sarah E.; Murray-Wijelath, Jacqueline; Wijelath, Errol S.

doi:10.1002/jbm.b.30436

Cited by 10 publications

(8 citation statements)

References 46 publications

(73 reference statements)

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“… 88 Interestingly, differential healing of various grafts in dogs was suggested to be implant site-dependent due to variations in fluid dynamics. 89 Taken together, these results highlight the importance of shear stress for in situ TE. For artificial heart valves, the shear stress profile depends on the valve’s geometrical design, 90 , 91 and as such represents an important design criterion.…”

Section: Modulating the Regenerative Response In The Hemodynamic Envimentioning

confidence: 66%

“…Small animals (mice, rats) in particular are being used extensively to answer preliminary research questions in the developmental phase of new prostheses (e.g., regarding biocompatibility, material choice, and device design). However, although the bulk of animal studies for in situ vascular regeneration reports on promising patency and endothelialization rates, both the underlying mechanisms and the timescales are subject to strong interspecies variations, as well as implant-site variations, 89 , 163 which may mislead their conclusions in terms of clinical application. It is often overlooked that the predictive value of an animal model is only as useful as the context in which it is interpreted.…”

Section: Current Challengesmentioning

confidence: 99%

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Biomaterial-driven in situ cardiovascular tissue engineering—a multi-disciplinary perspective

et al. 2017

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There is a persistent and growing clinical need for readily-available substitutes for heart valves and small-diameter blood vessels. In situ tissue engineering is emerging as a disruptive new technology, providing ready-to-use biodegradable, cell-free constructs which are designed to induce regeneration upon implantation, directly in the functional site. The induced regenerative process hinges around the host response to the implanted biomaterial and the interplay between immune cells, stem/progenitor cell and tissue cells in the microenvironment provided by the scaffold in the hemodynamic environment. Recapitulating the complex tissue microstructure and function of cardiovascular tissues is a highly challenging target. Therein the scaffold plays an instructive role, providing the microenvironment that attracts and harbors host cells, modulating the inflammatory response, and acting as a temporal roadmap for new tissue to be formed. Moreover, the biomechanical loads imposed by the hemodynamic environment play a pivotal role. Here, we provide a multidisciplinary view on in situ cardiovascular tissue engineering using synthetic scaffolds; starting from the state-of-the art, the principles of the biomaterial-driven host response and wound healing and the cellular players involved, toward the impact of the biomechanical, physical, and biochemical microenvironmental cues that are given by the scaffold design. To conclude, we pinpoint and further address the main current challenges for in situ cardiovascular regeneration, namely the achievement of tissue homeostasis, the development of predictive models for long-term performances of the implanted grafts, and the necessity for stratification for successful clinical translation.

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Section: Modulating the Regenerative Response In The Hemodynamic Envimentioning

confidence: 66%

Section: Current Challengesmentioning

confidence: 99%

Biomaterial-driven in situ cardiovascular tissue engineering—a multi-disciplinary perspective

et al. 2017

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“…A more complex model of vascular grafting will be needed to determine whether this enhanced cellular and capillary ingrowth will translate into a more rapid or complete endothelialization of the lumen of an ePTFE vascular graft. The role of fluid dynamics within the graft construct will also need to be examined, because bends in a graft construct can lead to less than optimal healing 26. Measurements of fibrous capsule formation and its effects on graft healing and endothelialization will also be required.…”

Section: Discussionmentioning

confidence: 99%

FTIR equivalent weight determination of perfluorosulfonate polymers

Perusich

2010

J of Applied Polymer Sci

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Fourier transform infrared (FTIR) and attenuated total reflectance (ATR) spectroscopy studies of the sulfonyl fluoride, potassium salt, and sulfonic acid forms of long and short side chain perfluorosulfonate polymers revealed bands indicative of the sidegroup and backbone compositions, endgroups on the main chain, water content, monomer concentration, and degree of salt hydrolysis. The equivalent weight (EW) of the polymer was obtained by titration and NMR measurements which were then calibrated to either the CAF/CAOAC absorbance band ratio for thin (<1.1 mil) films or to a CAF/SO 2 F absorbance band ratio for thick films (5 to 25 mils). An FTIR measurement of the film thickness based on the CAF group concentration was found to be both a function of the actual thickness and the EW; a method for compensating for this EW dependence is described. Esterification and fluorination of the polymers yielded FTIR measurements of the endgroup compositions on the polymer backbone which were shown to consist of ACOF, ACOOH, ACO 2 CH 3 , and ACF¼ ¼CF 2 groups. Thermogravimetric Analysis Infrared (TGA-IR) spectroscopy of the acid form polymers indicates that degradation begins by the decomposition of the ASO 3 H group at 320 C followed by bulk deterioration above 400 C. The FTIR techniques detailed herein have been developed for accurate, reproducible, and rapid compositional measurements of Nafion V R and other perfluorosulfonate polymers.

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“…38 The tube curvature could have been characterized and applied to the CAD model. 23 Both techniques were beyond the scope of this project.…”

Section: Study Limitationsmentioning

confidence: 99%

Effects of Thrombosed Vena Cava Filters on Blood Flow: Flow Visualization and Numerical Modeling

et al. 2008

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Inferior vena cava (IVC) filters are used to prevent pulmonary embolism (PE) in patients with deep vein thrombosis for whom anticoagulation is contraindicated. IVC filters have been shown to be effective in trapping embolized clots and preventing PE; however, among the commercially available designs, the optimal balance of clot capture efficiency, clot dissolution, and prevention of to vena cava occlusion is unknown. Clot capture efficiency has been quantified in numerous in vitro studies, in which model clots are released into a mock circulation system, with the relative capture efficiency of various IVC filters analyzed statistically. In general, two-stage filters have been found to be more efficient than one-stage filters. However, other factors may play a role in the ultimate dissolution of clots and in the overall effect of the resulting blood flow on caval vasculature. Clot dissolution has been shown to increase with increasing wall shear stress, while low and oscillating wall shear stresses are known to have a deleterious effect on vessel walls, causing intimal hyperplasia. This paper describes the effect of IVC filters on blood flow, velocity patterns, and wall shear stress by flow visualization and computational fluid dynamics.

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Role of fluid dynamics on the healing of an in vivo tissue engineered vascular graft

Cited by 10 publications

References 46 publications

Biomaterial-driven in situ cardiovascular tissue engineering—a multi-disciplinary perspective

Biomaterial-driven in situ cardiovascular tissue engineering—a multi-disciplinary perspective

FTIR equivalent weight determination of perfluorosulfonate polymers

Effects of Thrombosed Vena Cava Filters on Blood Flow: Flow Visualization and Numerical Modeling

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