Vascular endothelial growth factor induces anti-Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients

Yanqiu, Fang; Lü, Xiaodan; Liu, Lei; Lin, Xiuying; Sun, Munan; Fu, Jianhua; Xu, Shiwen; Tan, Yan

doi:10.3892/mmr.2016.5173

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…On the other hand, a positive correlation between intrafollicular AMH, AFC and AMH receptor (AMHR2) was also found. Coincidentally, previous studies showed that cows with high AFC had increased mRNA expression of AMHR2α compared with low AFC cows (Stojsin‐Carter et al., 2016; Rosa et al., 2018) and the expression of AMHR2 has been negatively correlated with follicular growth and oocyte maturation (Fang et al., 2016; Pierre et al., 2016). Therefore, findings from the current study and a growing body of literature suggest that cattle with lower circulating AMH could present greater LH concentrations, higher amounts of LH receptors and low AMHR2 expression, consequently affecting the growth pattern of dominant follicles.…”

Section: Discussionmentioning

confidence: 98%

Anti‐Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers

Batista¹,

Vieira²,

Freitas³

et al. 2020

Reprod Domestic Animals

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This study evaluated the association between plasma anti‐Mullerian hormone (AMH) concentration and fertility in Nelore (Bos indicus) heifers submitted to timed artificial insemination (TAI). At the onset of the synchronization protocol, heifers (n = 289) received a subcutaneous P4 ear implant (3 mg) and 2 mg of oestradiol benzoate. Eight days later, the P4 implant was removed and 0.5 mg of oestradiol cypionate, prostaglandin (0.265 mg, i.m.) and equine chorionic gonadotropin (300 UI, i.m.) was administered, and TAI was performed 48 hr after ear implant removal. Ovarian ultrasound evaluations were performed to measure number of ovarian follicles, dominant follicle size and ovulation response. Pregnancy diagnosis was performed by ultrasound 30 days after AI. Heifers with greater circulating AMH had more antral follicles, a smaller dominant follicle near timed ovulation and lower ovulation response to the timed AI protocol compared to heifers with lower circulating AMH. Although AMH and pregnancy outcome had a quadratic‐shaped pattern, AMH was not significantly associated with fertility. In conclusion, heifers with lower AMH had larger follicles towards the end of the synchronization protocol and greater ovulation responses, whereas greater circulating AMH was unrelated to conception success.

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Section: Discussionmentioning

confidence: 98%

Anti‐Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers

Batista¹,

Vieira²,

Freitas³

et al. 2020

Reprod Domestic Animals

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show abstract

“…GJA1 (CX43), which localizes in gap junctions between oocyte and CCs and participates in primordial follicle assembly, is downregulated in PCOS oocytes, suggesting that oocytes in PCOS patients may not connect well with the surrounding CCs33. Overexpression of AMHR2 increases the binding of AMH to it, which attenuates follicular or oocyte maturation34. Therefore, AMHR2 is a possible indicator for the quality of embryo35.…”

Section: Discussionmentioning

confidence: 99%

Single-cell analysis of differences in transcriptomic profiles of oocytes and cumulus cells at GV, MI, MII stages from PCOS patients

Liu

Feng

et al. 2016

Sci Rep

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Polycystic ovary syndrome (PCOS) is a common frequent endocrine disorder among women of reproductive age. Although assisted reproductive techniques (ARTs) are used to address subfertility in PCOS women, their effectiveness is not clear. Our aim was to compare transcriptomic profiles of oocytes and cumulus cells (CCs) between women with and without PCOS, and assess the effectiveness of ARTs in treating PCOS patients. We collected oocytes and CCs from 16 patients with and without PCOS patients to categorize them into 6 groups according to oocyte nuclear maturation. Transcriptional gene expression of oocyte and CCs was determined via single-cell RNA sequencing. The ratio of fertilization and cleavage was higher in PCOS patients than in non-PCOS patients undergoing ARTs, and there was no difference in the number of high-quality embryos between the groups. Differentially expressed genes including PPP2R1A, PDGFRA, EGFR, GJA1, PTGS2, TNFAIP6, TGF-β1, CAV1, INHBB et al. were investigated as potential causes of PCOS oocytes and CCs disorder at early stages, but their expression returned to the normal level at the metaphase II (MII) stage via ARTs. In conclusion, ARTs can improve the quality of cumulus-oocyte complex (COC) and increase the ratio of fertilization and cleavage in PCOS women.

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“…Oocytes from poorly vascularized follicles developed in morphologically inferior embryos as compared to those from well-vascularized follicles, indicating that vascularization of the follicles can be a potential marker for the developmental potential of an embryo (Nargund et al, 1996). Dysregulation of VEGF may attenuate follicular or oocyte maturation by stimulating AMHR2 overexpression, which increases AMH binding (Fang et al, 2016). These results suggest that ovarian angiogenesis is very deeply involved in oocyte maturation process.…”

Section: Discussionmentioning

confidence: 91%

Paeonia lactiflora improves ovarian function and oocyte quality in aged female mice

et al. 2020

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Although ovarian aging is a key cause of decreased ovarian function and oocyte quality, it remains a problem in infertility treatment. Therefore, this study is aimed to investigate whether Paeonia lactiflora (PL), a herb improves ovarian function and oocyte quality using aged female mice. C57BL/6 female mice aged 8 months were treated orally every day with PL of 26.5 mg/kg (n=7) and 53 mg/kg (n=7) of body weight for 4 weeks using an oral zoned needle. The control group (n=7) was treated with normal saline. Ovaries and serum were collected for the H&E stain and the evaluation of reactive oxygen species (ROS) levels, respectively. In the second experiment, female mice were orally administered with PL (26.5 mg/kg: n=12, 53 mg/kg: n=12, control: n=12) and then superovulated with PMSG and hCG, and mated with male mice. Zygotes were retrieved and cultured for 4 days. Ovaries were provided for examination of expressions of genes associated with angiogenesis (VEGF and visfatin), anti-aging (Sirt1 and Sirt2), and follicular development (c-Kit, BMP-15, and GDF-9). PL significantly increased numbers of surviving follicles (primordial, primary, secondary, and antral), numbers of zygotes retrieved, embryo development rate, and ovarian expression of VEGF, visfatin, c-Kit, BMP-15, and GDF-9 at both doses. However, ovarian expression of Sirt1 and Sirt2 was increased at 53.0 mg/kg of PL. ROS levels were not affected by PL. These results suggest that PL may possess beneficial effects regarding ovarian function and oocyte quality, possibly by activation of ovarian angiogenesis and follicular development.

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Vascular endothelial growth factor induces anti-Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients

Cited by 15 publications

References 26 publications

Anti‐Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers

Anti‐Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers

Single-cell analysis of differences in transcriptomic profiles of oocytes and cumulus cells at GV, MI, MII stages from PCOS patients

Paeonia lactiflora improves ovarian function and oocyte quality in aged female mice

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