Vascular colonization patterns in susceptible and resistant tomato cultivars inoculated with <i>Fusarium oxysporum</i> f.sp. <i>lycopersici</i> races 0 and 1

Rodríguez‐Molina, M. C.; Medina, Israel Lima; Torres‐Vila, Luis M.; Cuartero, J.

doi:10.1046/j.1365-3059.2003.00810.x

Cited by 12 publications

(12 citation statements)

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“…the fungus distribution was discontinuous in all combinations from 2-8 dpi, then continuous from 14-21 dpi with distinct patterns in the incompatible and compatible combinations. From 14 dpi onwards, symptoms became obvious in the compatible interaction as generally reported in the literature [49,11,20]. Whereas the two virulent strains fully colonize the stem, colonization by the avirulent strain is reduced, and at 18 and 21 dpi the height reached in stems is significantly lower than that reached at 2 and 4 dpi.…”

Section: Discussionsupporting

confidence: 74%

Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

et al. 2011

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BackgroundFusarium oxysporum f. sp. melonis Snyd. & Hans. (FOM) causes Fusarium wilt, the most important infectious disease of melon (Cucumis melo L.). The four known races of this pathogen can be distinguished only by infection on appropriate cultivars. No molecular tools are available that can discriminate among the races, and the molecular basis of compatibility and disease progression are poorly understood. Resistance to races 1 and 2 is controlled by a single dominant gene, whereas only partial polygenic resistance to race 1,2 has been described. We carried out a large-scale cDNA-AFLP analysis to identify host genes potentially related to resistance and susceptibility as well as fungal genes associated with the infection process. At the same time, a systematic reisolation procedure on infected stems allowed us to monitor fungal colonization in compatible and incompatible host-pathogen combinations.ResultsMelon plants (cv. Charentais Fom-2), which are susceptible to race 1,2 and resistant to race 1, were artificially infected with a race 1 strain of FOM or one of two race 1,2 w strains. Host colonization of stems was assessed at 1, 2, 4, 8, 14, 16, 18 and 21 days post inoculation (dpi), and the fungus was reisolated from infected plants. Markedly different colonization patterns were observed in compatible and incompatible host-pathogen combinations. Five time points from the symptomless early stage (2 dpi) to obvious wilting symptoms (21 dpi) were considered for cDNA-AFLP analysis. After successful sequencing of 627 transcript-derived fragments (TDFs) differentially expressed in infected plants, homology searching retrieved 305 melon transcripts, 195 FOM transcripts expressed in planta and 127 orphan TDFs. RNA samples from FOM colonies of the three strains grown in vitro were also included in the analysis to facilitate the detection of in planta-specific transcripts and to identify TDFs differentially expressed among races/strains.ConclusionOur data suggest that resistance against FOM in melon involves only limited transcriptional changes, and that wilting symptoms could derive, at least partially, from an active plant response.We discuss the pathogen-derived transcripts expressed in planta during the infection process and potentially related to virulence functions, as well as transcripts that are differentially expressed between the two FOM races grown in vitro. These transcripts provide candidate sequences that can be further tested for their ability to distinguish between races.Sequence data from this article have been deposited in GenBank, Accession Numbers: HO867279-HO867981.

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Section: Discussionsupporting

confidence: 74%

Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

et al. 2011

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“…Some proved highly susceptible such as Super-Strain B, Prichard, Peto 95 and Super-Magic; others were less susceptible to infection, such as Yara and Fayrose while, the remainders proved relatively resistant such as Super-Balady, Marmand, Super-Shahd and Super-Marmand. Results obtained in this investigation are in agreement with those found by many investigators in Egypt and other parts of the world (El-Zawahry, 1984;Moustafa and Khafagi, 1992;Moustafa, 1999;Rodriguez et al, 2003;Reis et al, 2005;Al-Khatib et al (2006); Abdalla, 2007;Yousef, 2007;El-Kazzaz et al, 2008;Kapoor, 2008;Amini, 2009;Dordevic et al, 2012;Steinkellner et al, 2012 andRadwan et al, 2016). Moreover, the following Egyptian varieties and hybrids, i.e.…”

Section: C Rapd-pcr Analysis Of Genetic Variation Among Isolates Osupporting

confidence: 91%

“…Fusarium oxysporum f.sp. lycopersici has previously been reported to be main cause of tomato wilt in Egypt (El-Zawahry, 1984, El-Shami, 1987, Farag, 2011and Sammour et al, 2013and Radwan et al 2016 and other parts of the world (Rodriguez et al, 2003, Song et al, 2004, Amini, 2009, Nijue et al, 2012, Jacobs et al, 2013, Enespa and Dwivedi, 2014and Hamini-Kadar et al, 2014. Pathogenicity tests are the primary means to distinguish different pathogenic Fusarium strains, but they do not indicate whether isolates of a given physiologic race or forma speciales are genetically related (Katan et al, 1989).…”

Section: C Rapd-pcr Analysis Of Genetic Variation Among Isolates Omentioning

confidence: 99%

Molecular Variations among some Isolates of Fusarium oxysporum f.sp lycopersici and Response of Different Tomato Cultivars and Seedling Age to Infection

Saleh

Gabr

Khalil

et al. 2016

Egyptian Journal of Phytopathology

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urvey of tomato wilt was carried out through two successive growing seasons (2013 and 2014) in farms' located at 4 sites belonging to four Egyptian Governorates (Behera, Minofiya, Ismailia and Minia). The disease incidence in these sites ranged between 14.3 in Ismailia Governorate and 37.5% in Minofiya Governorate. Isolation on Potato Agar medium from naturally infected plants collected from some Egyptian Governorates resulted in obtaining many fungal isolates that proved to be pathogenic to tomato Super-Strain B cultivar on pathogenicity test.The agent responsible for wilt of tomato in Egypt was identified as Fusarium oxysporum. The pathogenic capabilities of eight fungal isolates representing different localities in different Governorates, i.e. Behera, Minofiya, Ismailia and Minia were investigated., Pathogenicity test using eight isolates of Fusarium oxysporum isolated from naturally infected tomato plants showing typical symptoms of wilt revealed that they were all able to cause wilt symptoms on different tomato cultivars. In this respect, isolate of F. oxysporum isolated from El-Khatatba, Minofiya Governorate was the most virulent that induced the disease in tomato plants. Ten plant species were evaluated for their reactions to infection with the most virulent isolate, El-Khattba (Minofiya Governorate). The RAPD-PCR of the DNA analysis using 10-mer primer of eight isolates of F. oxysporum f.sp. lycopersici collected from different Governorates showed variations in DNA pattern. To test their response to infection with this isolate, ten tomato cultivars were evaluated to infection by the most virulent isolate (isolated from El-Khattba, Minofiya Governorate) and the results indicated that cv. Super-Strain B was the highest susceptible, whereas cv. Super-Marmand was the lowest one. Moreover, effect of tomato seedlings age of Super-Strain B (highly susceptible) and Super-Marmand (low susceptible) on infection by the most virulent isolate of the pathogen was tested.

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“…lycopersici Synder and Hansen, is the most important which causes serious economic loss in all tomato growing areas of the world. The management of the disease can be done through cultural practices (Katan, 1989), use of resistant varieties Rodriguez et al, 2003), chemical measures (Zindan et al, 2000), biological control (Gnanamanikram, 2002;Myresiotis et al, 2012;Bansal et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Sustainable Integrated Approach for Management of Fusarium Wilt of Tomato Caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) Synder and Hansen

Singh¹,

Biswas²,

Nagar³

et al. 2015

SAR

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Integration of different methods for sustainable management of Fusarium wilt of tomato revealed that all the treatments were able to significantly reduce the disease severity over control. Among the treatments, the minimum disease severity was recorded in the T 3 treatment where treatment was given as seed treatment with T. harzianum + soil application of neem cake powder + foliar spray of carbendazim, representing the value 4.82, 8.23, 12.37 and 16.37 per cent against 10.75, 14.84, 20.00 and 25.75 per cent in case of control at 30, 45, 60 and 75 days age of plant, respectively. Integrated approach has also been able to increase shoot length as 27.00, 42.15, 54.98, and 65.07 cm at 30, 45, 60 and 75 days age of plant, respectively whereas in case of control it was 18.60, 25.70, 34.63 and 44.29. Similarly, fresh and dry shoot and root weight have also been found increased in case of integrated approaches. Biochemical analysis of the treated plants revealed that highest soluble protein and total phenol content is observed in T3 treatment (Seed treatment with T. harzianum + soil treatment with neem cake powder and foliar spray with carbendazim), showing 37.85, 36.78 and 35.76 mg/gm of fresh leave of protein and 2.40, 2.14 and 2.04 mg/gm of fresh leave of phenol at 30, 45 and 60 days age of plant. As per yield is concerned, it has found that the maximum yield was obtained per plant from the treatment given as seed treatment with T. harzianum + soil treatment with neem cake powder + foliar spray with carbendazim, showing the value of 500 g per plant which was followed by the treatment given as seed treatment with T. harzianum + soil treatment with neem cake powder + foliar spray with neem leaf extract showing 470 g per plant.

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Vascular colonization patterns in susceptible and resistant tomato cultivars inoculated with Fusarium oxysporum f.sp. lycopersici races 0 and 1

Cited by 12 publications

References 12 publications

Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

Molecular Variations among some Isolates of Fusarium oxysporum f.sp lycopersici and Response of Different Tomato Cultivars and Seedling Age to Infection

Sustainable Integrated Approach for Management of Fusarium Wilt of Tomato Caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) Synder and Hansen

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