Vascular and Morphogenetic Abnormalities Associated with Exposure of Cigarette Smoke Condensate during Chicken and Murine Embryogenesis

Ejaz, Sohail; Ahmed, Ejaz; Sohail, Amara; Lim, Chae Woong

doi:10.1016/s0895-3988(10)60068-2

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…Our data suggested a probable common mechanism of several environmental factors causing craniofacial syndromes. We note that the molecular links between RA, hypoxia, and diabetes revealed in our study reflect those shown between the RA signaling pathway and cigarette smoking 61 – 63 or ethanol exposure 64 – 66 which are other environmental factors associated with embryonic defects. Finally, our models offer new target genes for craniofacial disorders and thus help to decipher molecular pathways involved in craniofacial development.…”

Section: Discussionsupporting

confidence: 78%

Prenatal retinoic acid exposure reveals candidate genes for craniofacial disorders

Berenguer¹,

Darnaudéry²,

Claverol³

et al. 2018

Sci Rep

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Syndromes that display craniofacial anomalies comprise a major class of birth defects. Both genetic and environmental factors, including prenatal retinoic acid (RA) exposure, have been associated with these syndromes. While next generation sequencing has allowed the discovery of new genes implicated in these syndromes, some are still poorly characterized such as Oculo-Auriculo-Vertebral Spectrum (OAVS). Due to the lack of clear diagnosis for patients, developing new strategies to identify novel genes involved in these syndromes is warranted. Thus, our study aimed to explore the link between genetic and environmental factors. Owing to a similar phenotype of OAVS reported after gestational RA exposures in humans and animals, we explored RA targets in a craniofacial developmental context to reveal new candidate genes for these related disorders. Using a proteomics approach, we detected 553 dysregulated proteins in the head region of mouse embryos following their exposure to prenatal RA treatment. This novel proteomic approach implicates changes in proteins that are critical for cell survival/apoptosis and cellular metabolism which could ultimately lead to the observed phenotype. We also identified potential molecular links between three major environmental factors known to contribute to craniofacial defects including maternal diabetes, prenatal hypoxia and RA exposure. Understanding these links could help reveal common key pathogenic mechanisms leading to craniofacial disorders. Using both in vitro and in vivo approaches, this work identified two new RA targets, Gnai3 and Eftud2, proteins known to be involved in craniofacial disorders, highlighting the power of this proteomic approach to uncover new genes whose dysregulation leads to craniofacial defects.

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Section: Discussionsupporting

confidence: 78%

Prenatal retinoic acid exposure reveals candidate genes for craniofacial disorders

Berenguer¹,

Darnaudéry²,

Claverol³

et al. 2018

Sci Rep

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“…Our study revealed that exposure to chemicals in e-cigarettes significantly decreased the survival likelihood in chicken embryos as 76% of chicken embryos in the 60 μg ECL group died compared to only 11% from their matched controls. This finding is consistent with the current literature on the effects of traditional cigarette smoking on embryo survival [ 23 ]. Other studies have elucidated the harmful effects of ECL on embryogenesis albeit by using different animal models.…”

Section: Discussionsupporting

confidence: 93%

“…To our knowledge, no studies investigated the effects of ECL on embryo survival and its effects on the early stages of embryogenesis including angiogenesis. Therefore, we decided to use the chicken embryo model as it has been used to study similar parameters in previous studies [ 18 , 23 ]. Our study revealed that exposure to chemicals in e-cigarettes significantly decreased the survival likelihood in chicken embryos as 76% of chicken embryos in the 60 μg ECL group died compared to only 11% from their matched controls.…”

Section: Discussionmentioning

confidence: 99%

E-Cigarette Liquid Provokes Significant Embryotoxicity and Inhibits Angiogenesis

Ashour

Alhussain

Rashid

et al. 2020

Toxics

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E-cigarette smoking (ECS) is a new method of tobacco smoking that is gaining popularity as it is thought to be a “healthy method” of tobacco consumption. The adverse outcomes of ECS on the respiratory and cardiovascular systems in humans have been recently demonstrated. Nevertheless, the effect of e-cigarette liquid (ECL) on the early stage of embryogenesis and angiogenesis has not been explored yet. Chicken embryo at 3 days of incubation and its chorioallantoic membrane (CAM) of 5 days were used to explore the outcome of ECL on the embryo. Real-time PCR was also employed to study the regulation of a set of key controller genes of embryogenesis as well as angiogenesis. Our study revealed that ECL exposure is associated with a high rate of mortality in embryos as around 70% of treated embryos, at 3 days of incubation, die after 5 days of exposure. Additionally, ECL inhibits angiogenesis of the CAM of 5 days of incubation by more than 30%. These effects could be explained by the upregulation of ATF-3, FOXA2, INHBA, MAPRE-2, and RIPK-1, as well as the downregulation of SERPINA-4 and VEGF-C genes, which are important key controller genes of embryogenesis as well as angiogenesis. Our data suggest clearly that ECS can have dramatic toxic outcomes on the early stage of embryogenesis as well as angiogenesis. Accordingly, we believe that further studies to assess the effects of ECS on human health are essential.

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“…The results were evaluated as CSP and expressed in terms of means ± S.D. Analysis of variance (ANOVA) and Post hoc t-test was applied to analyze data and P < 0.05 was considered as significant [34,37].…”

Section: Discussionmentioning

confidence: 99%

Exploration of the in vitro cytotoxic and antiviral activities of different medicinal plants against infectious bursal disease (IBD) virus

et al. 2014

Self Cite

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Infectious bursal disease (IBD) caused by non-enveloped double stranded RNA virus is an acute and contagious poultry disease. Outbreak of IBD could result in 10-75% mortality of the birds; hence it has gained socio-economic importance worldwide. Medicinal plants have shown broad spectrum anti-viral activities against RNA and DNA viruses. Moringa oleifera Lam (MOL), Phyllanthus emblicus Linn (PEL), Glycyrrhiza glabra Linn (GGL), and Eugenia jambolana Lam (EJL) are commonly available medicinal plants of the sub-continent and exhibited anti-viral potential against different viruses. Ethanolic extracts of the leaves of MOL and EJL, roots of GGL and dried fruit of PEL were investigated for their cytotoxic and anti-viral potential against IBD virus using MTT colorimetric assay and anti-viral assay. Significant anti-viral potential (P<0.001) was demonstrated at concentrations 12.5, 25, 50 and 100 µg ml -1 of GGL, PEL, MOL and EJL, respectively, with no cytotoxicity. Data also spotlighted that all tested plant extracts possess significant anti-viral potential and this trend was higher in GGL followed by PEL, MOL, and EJL. The data undoubtedly conclude that these medicinal plants contain several health beneficial phyto-chemicals which got significant anti-viral potential and effectively be utilized against IBD virus. Moreover, the outcomes of this study provide a platform on the way to discover novel anti-viral agents against IBD virus and other viruses from plant origin.

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Vascular and Morphogenetic Abnormalities Associated with Exposure of Cigarette Smoke Condensate during Chicken and Murine Embryogenesis

Cited by 10 publications

References 33 publications

Prenatal retinoic acid exposure reveals candidate genes for craniofacial disorders

Prenatal retinoic acid exposure reveals candidate genes for craniofacial disorders

E-Cigarette Liquid Provokes Significant Embryotoxicity and Inhibits Angiogenesis

Exploration of the in vitro cytotoxic and antiviral activities of different medicinal plants against infectious bursal disease (IBD) virus

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