Exploration of the in vitro cytotoxic and antiviral activities of different medicinal plants against infectious bursal disease (IBD) virus

Ahmad, Waheed; Ejaz, Sohail; Anwar, Khaleeq; Ashraf, Muhammad

doi:10.2478/s11535-013-0276-8

Cited by 13 publications

(11 citation statements)

References 54 publications

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“…Previous research showed that jamun fruit crude extract exhibited cytotoxic activity against MCF 7, Hela Cervic carcinoma cell lines, Hep G2 liver carcinoma cell lines, H 460 lung carcinoma cell lines, and U251 brain carcinoma cell lines 21,22 , but not on hPBMCs. Waqas Ahmad et al, 23 used Jamun leaf powder to study the cytotoxic effects on Vero cell lines with a maximum concentration of 100 µg/mL, and almost agreement percent cell survival was noted in our study also 24 .…”

Section: Discussionsupporting

confidence: 78%

Untitled

2020

IJPSR

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In nature, lectins or glycoproteins are ubiquitous, abundantly distributed. Lectinsplayan an important role in plant defense mechanism against microorganisms. This fact has greatly attracted many research groups working in the field of biotechnology, agriculture, and medicine. Jamun or black plum is a fruit with many health benefits. This paper focuses on the phytochemical investigation of trichloroacetic acid precipitated proteins from leaves, pulp, and seeds. The glycoproteins were characterized by hemagglutination, hemagglutination inhibition, and tested the stability at different temperatures and pH on blood groups by and tested their efficacy on human Peripheral Blood Mononuclear Cells. Phytochemical investigations of trichloroacetic acid precipitated test sample defined the occurrence of protein and absence of other phytochemicals. Blood group O + showed a quick response of agglutination. Both pulp and leaf extracts showed cytotoxicity and genotoxicity on human Peripheral Blood Mononuclear Cells in a dosedependent manner. Glycoproteins were stable at varying temperatures and pH. The current study results indicated that the jamun fruit extract is also a potent anti-proliferative agent against lymphocytes. However, further tests have to be done to investigate the appropriate dose, toxicity, and mechanism of anti-proliferative action of jamun pulp on human Peripheral Blood Mononuclear Cells.

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Section: Discussionsupporting

confidence: 78%

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2020

IJPSR

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“…Ethanolic extract of leaves showed negative activity for herpes simplex virus (HSV; Lipipun et al, ) and seeds against HSV‐1 (Ali, El‐Taweel, & Ali, ). Ethanolic extract of leaves showed significant antiviral activity against infectious bursal disease virus (Ahmad et al, ), foot and mouth disease virus, equine herpes virus, hepatitis virus, and rhinovirus (Younus et al, ). Leaf aqueous extract activated the cellular immunity in infected mice with HSV‐1 by decreasing the infection rate and reducing herpetic skin lesion growth (Kurokawa et al, ).…”

Section: Resultsmentioning

confidence: 99%

Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

Dhakad

Ikram

Sharma

et al. 2019

Phytotherapy Research

169

100

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The genus Moringa Adans. comprises 13 species, of which Moringa oleifera Lam. native to India and cultivated across the world owing to its drought and frost resistance habit is widely used in traditional phytomedicine and as rich source of essential nutrients. Wide spectrum of phytochemical ingredients among leaf, flower, fruit, seed, seed oil, bark, and root depend on cultivar, season, and locality. The scientific studies provide insights on the use of M. oleifera with different aqueous, hydroalcoholic, alcoholic, and other organic solvent preparations of different parts for therapeutic activities, that is, antibiocidal, antitumor, antioxidant, anti‐inflammatory, cardio‐protective, hepato‐protective, neuro‐protective, tissue‐protective, and other biological activities with a high degree of safety. A wide variety of alkaloid and sterol, polyphenols and phenolic acids, fatty acids, flavanoids and flavanol glycosides, glucosinolate and isothiocyanate, terpene, anthocyanins etc. are believed to be responsible for the pragmatic effects. Seeds are used with a view of low‐cost biosorbent and coagulant agent for the removal of metals and microbial contamination from waste water. Thus, the present review explores the use of M. oleifera across disciplines for its prominent bioactive ingredients, nutraceutical, therapeutic uses and deals with agricultural, veterinarian, biosorbent, coagulation, biodiesel, and other industrial properties of this “Miracle Tree.”

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“…Probably, the reduction observed in the pgRNA of genotype H was not enough to reduce the rate of replication of the virus, but the antifibrotic and antioxidant effects deduced by the changes in CTGF , TGFβ , and CAT gene expressions with M. oleifera treatment support the fact that Moringa might be used as a valuable nutritional supplement for patients with chronic hepatitis B. There are additional studies, both in vivo and in vitro , reporting effects of M. oleifera in different viruses, like herpes simplex virus type 1 [ 20 ], Epstein-Barr virus [ 21 ], Newcastle disease virus [ 22 ], and infectious bursal disease virus [ 23 ]. Nevertheless, the complete elucidation of the molecular mechanisms driving M. oleifera action remains to be defined.…”

Section: Discussionmentioning

confidence: 96%

Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells

Feustel

Ayón‐Pérez

Sandoval-Rodríguez

et al. 2017

Journal of Immunology Research

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Chronic hepatitis B infection treatment implicates a long-lasting treatment. M. oleifera extracts contain compounds with antiviral, antioxidant, and antifibrotic properties. In this study, the effect of M. oleifera was evaluated in Huh7 cells expressing either HBV genotypes C or H for the antiviral, antifibrotic, anti-inflammatory, and antioxidative responses. Huh7 cells were treated with an aqueous extract of M. oleifera (leaves) at doses of 0, 30, 45, or 60 μg/mL. The replicative virus and TGF-β1, CTGF, CAT, IFN-β1, and pgRNA expressions were measured by real time. HBsAg and IL-6 titers were determined by ELISA. CTGF, TGF-β1, IFN-β1, and pgRNA expressions decreased with M. oleifera treatment irrespective of the HBV genotype. HBsAg secretion in the supernatant of transfected Huh7 cells with both HBV genotypes was decreased regardless of the dose of M. oleifera. Similar effect was observed in proinflammatory cytokine IL-6, which had a tendency to decrease at 24 hours of treatment. Transfection with both HBV genotypes strongly decreased CAT expression, which is retrieved with M. oleifera treatment. M. oleifera treatment reduced fibrosis markers, IL-6, and HBsAg secretion in HBV genotypes C and H. However, at the level of replication, only HBV-DNA genotype C was slightly reduced with this treatment.

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Exploration of the in vitro cytotoxic and antiviral activities of different medicinal plants against infectious bursal disease (IBD) virus

Cited by 13 publications

References 54 publications

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Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells

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