Varying genetic diversity of Papaya ringspot virus isolates from two time-separated outbreaks in Jamaica and Venezuela

Chin, Mario P. S.; Rojas, Yndra Cordero de; Moret, Jaume Vicent Jordá; Fermín, Gustavo; Tennant, Paula

doi:10.1007/s00705-007-1035-9

Cited by 12 publications

(13 citation statements)

References 24 publications

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“…Similarly to Indian isolates, Puerto Rican isolates grouped independently of their geographical origin, although a higher level of conservation was observed among the latter isolates (Zambrana-Echevarr ıa et al, 2016). In this work, genetic variation was detected between isolates collected from the same areas (Boyeros-1 and -2, Melena del Sur-1 and -2, Santo Domingo-1 and -2), on different collection dates (2-6 years), which coincided with observations of Chin et al (2007). These authors analysed sequences collected from Jamaica and Venezuela in different periods and associated the variability with the movement of germplasm and the implemented management practices.…”

Section: Discussionsupporting

confidence: 61%

“…In this work, genetic variation was detected between isolates collected from the same areas (Boyeros‐1 and ‐2, Melena del Sur‐1 and ‐2, Santo Domingo‐1 and ‐2), on different collection dates (2–6 years), which coincided with observations of Chin et al . (). These authors analysed sequences collected from Jamaica and Venezuela in different periods and associated the variability with the movement of germplasm and the implemented management practices.…”

Section: Discussionmentioning

confidence: 97%

“…Gibbs et al (2008) provided evidence of the Asian origin of PRSV and proposed that the introduction of the virus to the Americas occurred about 300 years ago; a similar hypothesis was formulated by Olarte-Castillo et al (2011). Moreover, compared with the PRSV isolates in America (Chin et al, 2007;Zambrana-Echevarr ıa et al, 2016), genetic diversity of PRSV populations is highest in India (Jain et al, 2004), which coincides with the origin of this virus (Bateson et al, 2002). Several investigations propose the use of transgenic plants for PRSV management, although it requires a high homology between the transgene and the challenged virus coat protein (CP) gene (Fermin et al, 2010).…”

Section: Introductionmentioning

confidence: 85%

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Distribution and phylodynamics of papaya ringspot virus on Carica papaya in Cuba

et al. 2018

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Orchard and garden papaya crops grown in 47 Cuban municipalities were surveyed from 2008 to 2013, revealing the widespread distribution of papaya ringspot virus (PRSV) in Cuba. Phylodynamic analyses performed with the partial coat protein gene of all Cuban PRSV‐P isolates (34 sequences) and 107 sequences of isolates from the American continent and the Caribbean islands showed a most recent common ancestor in 1942 (95% highest posterior density, HPD 95% = 1911–1967). The substitution rate was estimated to be 7.7 × 10−4 substitutions per site per year (HPD 95% = 4.6 × 10−4 to 1.1 × 10−3), which is equivalent to those detected in other RNA viruses. Demographic reconstruction of PRSV showed that viral diversity increased in the 1985–1990 period, which coincides with the implementation of extensive production practices. Moreover in Cuba, viral dispersion occurred from Mexico and other unknown ancestral locations. The spatiotemporal diffusion analysis proposed Mexico as an ancestral area for the origin of diversification in the American continent and suggests new dispersion events between American and Caribbean isolates. The observed widespread distribution, clear geographic grouping of Cuban isolates, virus growth and genetic diversity provide strong evidence of the PRSV dispersion patterns, which has implications for the control strategies of PRSV.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 85%

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Distribution and phylodynamics of papaya ringspot virus on Carica papaya in Cuba

et al. 2018

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“…The results based on the CP nucleotide sequences also showed that the isolates were distributed into different groups according to the region of origin. Chin et al [33] also detected a high genetic variability among the PRSV isolates from Venezuela compared to isolates from Jamaica. Such differences could be related, among other factors, to geographic isolation [34].…”

Section: Resultsmentioning

confidence: 94%

Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba

Martínez¹

2014

AJAF

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The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country.

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“…ns, non-significant; **significant (P < 0AE01). (Azzam et al, 2000;Bateson et al, 2002;Chin et al, 2007). It should be noted that the genetic flow between Andalucía and surrounding areas may be strongly constrained by mountain barriers.…”

Section: Discussionmentioning

confidence: 99%

Maize dwarf mosaic virus diversity in the Johnsongrass native reservoir and in maize: evidence of geographical, host and temporal differentiation

2010

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The genetic diversity and population structure of Maize dwarf mosaic virus (MDMV) was examined by analysis of the full coat protein gene of 539 isolates collected from maize and Johnsongrass (Sorghum halepense) from eight different maizegrowing areas in Spain. Restriction fragment length polymorphism analysis revealed that the MDMV population consisted of 49 genetic variants, with the three most frequent accounting for 44% of the isolates. This population was spatially structured according to the establishment of maize crops in the area. The highest nucleotide diversity values were observed in the old maize-growing areas in the northeast of Spain (>0AE211) and the lowest in the new maize-growing areas in the west (<0AE019). Moreover, the major genetic variants differed between the old and new maize-growing areas. Evidence of hostassociated selection was found in the endemic area of the virus (Lleida), and aphid-transmission studies suggested vector selection pressure. Assessment of the temporal evolution of the MDMV population in northeastern areas indicated that time was only significant in Lleida, where it explained 4AE8% of the total variation over nine consecutive years.

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Varying genetic diversity of Papaya ringspot virus isolates from two time-separated outbreaks in Jamaica and Venezuela

Cited by 12 publications

References 24 publications

Distribution and phylodynamics of papaya ringspot virus on Carica papaya in Cuba

Distribution and phylodynamics of papaya ringspot virus on Carica papaya in Cuba

Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba

Maize dwarf mosaic virus diversity in the Johnsongrass native reservoir and in maize: evidence of geographical, host and temporal differentiation

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