Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

Abstract

Lipases are enzymes that catalyze various types of reactions and have versatile applications. Additionally, lipases are the most widely used class of enzymes in biotechnology and organic chemistry. Lipases can be produced by a wide range of organisms including animals, plants and microorganisms. Microbial lipases are more stable, they have substrate specificity and a lower production cost as compared to other sources of these enzymes. Although commercially available lipases are widely used as biocatalysts, there are still many challenges concerning the production of microbial lipases with the use of renewable sources as the main component of microbial growth medium such as straw, bran, oil cakes and industrial effluents. Submerged fermentation (SmF) and solid-state fermentation (SSF) are the two important technologies for the production of lipases by microorganisms. Therefore, this review focuses on microbial lipases, especially their function, specificity, types and technology production, including the use of renewable agro-industrial residues and waste materials.

Optimization and characterization of alkaliphilic lipase from a novel Bacillus cereus NC7401 strain isolated from diesel fuel polluted soil

“…Relatively less activity of 28.5 UmL -1 was reported at optimum pH (8.0) for lipase purified from B. cereus PCSIR NL-37 grown in a medium containing peptone [23] when compared to our results.…”

“…methylotrophicus PS3 and B . cereus PCSIR NL-37 respectively [ 23 , 43 ]. Lipase activity was relatively stable in the presence of β-marcaptoethanol.…”

“…Different researchers found various carbon sources such as soybean, corn, sunflower, olive [21], palm and cottonseed oils, wheat bran or soybean bran, triolein, and oleic acid which support the growth and production of lipase [22][23][24][25]. Other studies also found that the glucose supplementation to the basal medium acts as an inhibitor of lipase due to the catabolic repression.…”

Enhanced Erinacine A Production by Hericium erinaceus Using Solid-State Cultivation

“…Therefore, the corn kernel was selected as the substrate for the following experiments. The advantage of solid-state cultivation is that the source of the substrate is convenient, such as agri-food waste or natural food crops [34,35]. Since whole grains are obtained easily and at a low cost, six different grains were selected as the substrates: polished rice, corn kernel, adlay kernel, brown rice, red beans, and sesame.…”

Optimization of Submerged Fermentation Parameters for Enhanced Lipase Production from Newly Isolated Lipolytic Yeasts using Agro Waste Substrates

“…The cultivated microorganisms metabolize the nutrients in the liquid medium, grow and release the desired enzymes into the medium [1]. When the selected strain produces extracellular enzyme, it makes purification and recovery easier than when it is produced intracellular enzyme [13]. Most industries employ submerged fermentation for enzyme production due to the ease of handling on a large-scale [14,1].…”