Variations of genotypes of Vicia species as influenced by seed phenolic compounds and antioxidant activity

Orak, H. H.; Karamać, Magdalena; Amarowicz, Ryszard; Orak, Adnan; Janiak, Michał; Tenikecier, Hazim Serkan

doi:10.13080/z-a.2022.109.005

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…The study of the variability of selected parameters of crops depends on many factors among which the genotype and seasonal aspects that can be helpful to create high-quality plant products (Pšenakova et al 2010;Orak et al 2022). The study of A. tridentata Mutall spp.…”

Section: Discussionmentioning

confidence: 99%

Genotype Variation of Polyphenol Content and Antioxidant Activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd

Vergun,

Svydenko,

Grygorieva

et al. 2023

Agriculture (Pol'nohospodárstvo)

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The results of seasonal variation of total polyphenols (TPC), flavonoids (TFC) and phenolic acid (TPAC) accumulation and antioxidant activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd. genotypes from the Kherson Oblast of Ukraine represented in this study. The total polyphenol content was identified by the Folin-Ciocalteu method and ranged from 20.51 to 132.52 mg GAE/g (gallic acid equivalent). The flavonoid content conducted with aluminum chloride reagent ranged from 8.16 to 70.77 mg QE/g (quercetin equivalent) and phenolic acid content determined with Arnova reagent ranged from 6.26 to 38.67 mg CAE/g (caffeic acid equivalent) depending on genotype and stage of growth. The antioxidant activity of investigated genotypes determined by the DPPH (2.2-diphenyl-1-picrylhydrazyl) method (FRSA) varied from 4.68 to 10.38 mg TE/g (Trolox equivalent), molybdenum-reducing power of extracts detected by the phosphomolybdenum method (MRP) and was from 41.43 to 209.93 mg TE/g depending on genotype. The total polyphenol content, flavonoids and antioxidant activity by the phosphomolybdenum method increased from spring vegetation to flowering. The accumulation of phenolic acids and antioxidant activity by the DPPH method was uneven and depended on genotype and stage of growth. It was found a weak correlation between FRSA and TPC (r = 0.189), TFC (r = 0.211), and TPAC (r = 0.111) for genotypes. A strong correlation was found between MRP and TPC (r = 0.703), and TFC (r = 0.657). A moderate correlation was detected between MRP and TPAC (r = 0.481) for genotypes. The obtained data can be used for further breeding work to get genotypes with high antioxidant activity.

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Section: Discussionmentioning

confidence: 99%

Genotype Variation of Polyphenol Content and Antioxidant Activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd

Vergun,

Svydenko,

Grygorieva

et al. 2023

Agriculture (Pol'nohospodárstvo)

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“…The phenolic compounds were extracted from freeze-dried and ground flax using a methanol:water (5:1, v / v ) mixture. The extraction was carried out at a 1:10 ( v / w ) plant material-to-solvent ratio in closed glass vessels that were shaken in an SW22 water-bath (Julabo, Seelbach, Germany) at 65 °C [ 48 ]. A portion of 5 g of the freeze-dried and powdered flax was used for each extraction.…”

Section: Methodsmentioning

confidence: 99%

Phenolic Compound Profile and Antioxidant Capacity of Flax (Linum usitatissimum L.) Harvested at Different Growth Stages

et al. 2023

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The profile of phenolic compounds changes during the growth of a plant and this change affects its antioxidant potential. The aim of this research has been to find the growth stage of flax with the highest antioxidant capacity, and to determine the phenolic compounds responsible for such a capacity. Flax was harvested in six growth stages: from stem extension to mature seeds. The phenolic compounds were identified using LC–TOF–MS and quantified in an extract and in the fresh matter (FM) of each growth stage. The radical scavenging activity against ABTS•+ and DPPH•, the ferric-reducing antioxidant power (FRAP), and the antioxidant activity in the β-carotene-linoleic acid emulsion system were determined. Mono- and di-C-glycosyl flavones were found to be the most abundant phenolics of the aerial parts of flax, which also showed the highest content of isoorientin (210–538 µg/g FM). Coniferin, its derivative, and hydroxycinnamic acid derivatives were also detected. The plant was richer in flavone C-glycosides from stem extension to seed ripening (1105–1413 µg/g FM) than at the mature seed stage (557 µg/g FM). Most of the individual flavone C-glycoside contents in the extracts decreased when increasingly older plants were considered; however, the isoorientin content did not change significantly from the steam extension to the seed ripening stages. The antiradical activity against ABTS•+ and FRAP was higher for the aerial parts of the flax harvested at the flowering, brown capsule, and seed ripening stages, mainly due to the presence of flavone C-glycosides. The oxidation of β-carotene-linoleic acid emulsion was instead inhibited more effectively by the extracts from plants at the brown capsule and mature seed stages. Coniferin and its derivative were significantly involved in this activity. The extracts from the aerial parts of the flax harvested from flowering to seed ripening could be a valuable source of flavone C-glycosides for use as nutraceuticals and components of functional foods.

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“…Seeds were ground in a coffee mill (Bosch & Siemens Hausgeräte GmbH, Munich, Germany) into powder with a particle size <0.8 mm and defatted with hexane in a Soxhlet apparatus for 6–8 h. Powdered defatted material was extracted using 80% ( v / v ) methanol at a solid to solvent ratio of 1:10 ( w / v ) at 65 °C for 15 min using an SW22 water bath (Julabo, Seelbach, Germany) [ 47 , 48 ]. The process was carried out three times, and the supernatants from each step were pooled.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Antioxidant and Antimicrobial Activity and Phenolic Compound Profile of Extracts from Seeds of Different Vitis Species

et al. 2023

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Seeds of Vitis vinifera L. with a high content of bioactive compounds are valuable by-products from grape processing. However, little is known about the bioactivity of seeds from other Vitis species. The aim of this study has been to compare the phenolic composition, antimicrobial activity, and antioxidant activity of extracts from seeds of four Vitis species (V. riparia Michx., V. californica Benth., V. amurensis Rupr., and V. vinifera L.). Antioxidant activities were assessed as ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) scavenging activity, and oxygen radical absorbance capacity (ORAC). The antimicrobial activity was determined using the microdilution method against some Gram-negative (Escherichia coli, Salmonella enterica ser. Typhimurium, and Enterobacter aerogenes) and Gram-positive (Enterococcus faecalis and Staphylococcus aureus) bacteria. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to evaluate the phenolic profile of extracts. Flavan-3-ols, procyanidins, phenolic acids, flavonols, anthocyanins, and stilbenoids were detected. (+)-Catechin and (−)-epicatechin turned out to be the most abundant in the phenolic profile of V. amurensis seed extract. Phenolic acids prevailed in the extract from V. vinifera seeds. The V. riparia and V. californica seed extracts had higher contents of most individual phenolics compared to the other Vitis species. They also showed a higher total phenolic content, DPPH• scavenging activity, ORAC, and overall antibacterial activity. Total phenolic content significantly correlated with antioxidant activity and antimicrobial activity against E. coli. The principal component analysis (PCA) showed discrimination between V. vinifera, V. amurensis, and clustered V. riparia and V. californica with respect to variables. To recapitulate, this research demonstrates that seeds of different Vitis species, especially V. riparia and V. californica, are sources of molecules with antioxidant and antimicrobial activities that can be used in different sectors, such as in the food, cosmetic, and pharmaceutical industries.

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Variations of genotypes of Vicia species as influenced by seed phenolic compounds and antioxidant activity

Cited by 4 publications

References 29 publications

Genotype Variation of Polyphenol Content and Antioxidant Activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd

Genotype Variation of Polyphenol Content and Antioxidant Activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd

Phenolic Compound Profile and Antioxidant Capacity of Flax (Linum usitatissimum L.) Harvested at Different Growth Stages

Characterization of Antioxidant and Antimicrobial Activity and Phenolic Compound Profile of Extracts from Seeds of Different Vitis Species

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