Variations in Strains of Streptomyces griseus Isolated from a Degenerating Streptomycin-Producing Culture

Abstract

Brunswick, N.J.). Variations in strains of Streptomyces griseus isolated from a degenerating streptomycin-producing culture. Appl. Microbiol. 11:84-89. 1963.-Streptomycin-resistant strains were isolated from a degenerated streptomycin-producing culture of Streptomyces griseus. From 250 resistant strains, 3 low, 2 intermediate, and 2 high potency strains were selected; these were compared in their morphological, cultural, physiological, and streptomycin-producing properties. Though no definite correlation between streptomycin production and the other properties could be obtained, the following correlations were considered as distinct differences among the low, intermediate, and high potency strains. (i) When streptomycin-producing ability degenerates, more sub

Biochemistry and regulation of streptomycin and mannosidostreptomycinase (alpha-D-mannosidase) formation.

“…Some of the reported changes accompanying the shift to nonproducing cultures include increases in growth rate and sugar utilization rate (103), a gain of ability to use arabinose for growth (121), a decrease in hexose content and an increase in pentose and glucosamine content of cell walls (9,119), an increase in susceptibility to lysozyme, and an increase in resistance to disruption by sonic energy (9). Degenerated cultures have also been reported to appear more wrinkled on agar and to produce less of a soluble yellow pigment (86).…”

“…The two amidino groups attached to the streptamine nitrogens are derived by transamidination from arginine. With some strains, addition of arginine and inositol to fermentation media increases streptomycin yields (35,42,72,75,86,111); streptidine has also been reported to stimulate production (86). Synthesis of streptomycin by washed suspensions may also be stimulated by arginine and inositol and by streptidine or streptobiosamine (75,85).…”

STREPTOMYCIN FORMATION BY INTACT MYCELIUM OF STREPTOMYCES GRISEUS

“…Strain and inoculum. S. griseus 107 (Nomi, 1963) derived from S. griseus Z 38 was used in all experiments. One loopful of aerial mycelium from a slant culture on glucose-yeast extract agar was inoculated in 10 ml of the same medium without agar in a 50-ml Erlenmeyer flask and 1220 on September 29, 2020 by guest http://jb.asm.org/ Downloaded from cultured on a reciprocating shaker (98 rev/min) at 28 C for 2 days.…”

“…the growing-culture condition in glucose-yeast mg in the case of 24-hr-old mycelium, 3.8 mg in the extract medium (Nomi, 1963). Figure 1 shows case of 48-hr-old mycelium, and 4.58 mg in the case streptomycin production by the washed mycelium of 72-hr-old mycelium.…”