Biochemistry and regulation of streptomycin and mannosidostreptomycinase (alpha-D-mannosidase) formation.

Demain, Arnold L.; Inamine, E

doi:10.1128/mmbr.34.1.1-19.1970

Cited by 53 publications

(18 citation statements)

References 60 publications

(112 reference statements)

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“…The syntheses of alkaline phosphatases are derepressed in low-Pi environments (11). Possible roles of alkaline phosphatases and of dephosphorylation have been suggested in the formation of such secondary metabolites as streptomycin, lincomycin, viomycin, vancomycin, and pyocyanine (2,8,14). With the latter two, alkaline phosphatase activity was observed to increase just before the onset of synthesis of the secondary material; in this study, we made the same observation with prodigiosin and also showed that the increased activity failed to occur in noninduced cells.…”

Section: Discussionmentioning

confidence: 99%

Phosphate inhibition of secondary metabolism in Serratia marcescens

Witney¹,

Failla²,

Weinberg³

1977

Appl Environ Microbiol

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The synthesis of prodigiosin by non-proliferating cells of Serratia marcescens was examined in the presence of a wide range of concentrations of inorganic phosphate (Pi). A high elevation of pigment formation was obtained at-0.3 mM, and a broader but much lower elevation was obtained at 10 to 250 mM Pi. The synthesis of two immediate precursors of the pigment also was inhibited by Pi. The mechanism of action of Pi did not involve changes in pH or accumulation of the trace metal nutrient iron or zinc. Inhibition was most pronounced when Pi was added to the induction system before the onset of pigment formation. The inhibitor also diminished the burst of alkaline phosphatase activity that occurred in the period befween the start of induction and appearance of prodigiosin.

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Section: Discussionmentioning

confidence: 99%

Phosphate inhibition of secondary metabolism in Serratia marcescens

Witney¹,

Failla²,

Weinberg³

1977

Appl Environ Microbiol

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“…Samples of the culture were removed, centrifuged, and washed once with 0.85% NaCl. The mycelium was suspended in 3 to 4 volumes of cold deionized water and disrupted in a Branson sonifier (Heat Systems Co.) for 2 min in four 30-sec bursts. The disrupted suspensions were then centrifuged at 12,000 rev/min for 20 min in a refrigerated Sorvall RC-2 centrifuge to remove intact cells and cellular debris.…”

mentioning

confidence: 99%

Regulation of Secondary Metabolite Biosynthesis: Catabolite Repression of Phenoxazinone Synthase and Actinomycin Formation by Glucose

1972

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Synthesis of the secondary metabolite, actinomycin, and the enzyme, phenoxazinone synthase, involved in the biosynthesis of the antibiotic, were shown to be under severe catabolite repression by glucose. Of a variety of hexoses and carbon compounds examined, glucose, and to a lesser extent, mannose, proved to be the most repressive for enzyme synthesis. The repression by glucose was most evident before production of the antibiotic. In a chemically defined medium suitable for actinomycin production, synthesis of phenoxazinone synthase began at the time the glucose (0.1%) supply was depleted. Soon after, antibiotic synthesis was initiated. Galactose, the major carbon source for growth and antibiotic synthesis, was not utilized until the glucose was consumed. Generally, carbon compounds which supported a rapid rate of growth were most effective in producing catabolite repression.

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“…A. parasiticus ATCC 15517 (formerly known as A. flavus) was used throughout the study and was maintained as a soil culture. Five-to- 7 day-old spores, obtained from a bottle containing 50 ml of glucose-peptone-agar, were distributed equally to five 500-ml Erlenmeyer flasks containing 100 ml of sterile basal medium. These flasks were incubated at 26 i 1 C on a rotary shaker or as stationary cultures for 8 days unless otherwise stated.…”

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confidence: 99%

High Aflatoxin Production on a Chemically Defined Medium 1

Reddy

Viswanathan

Venkitasubramanian

1971

Applied Microbiology

107

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Aspergillus parasiticus ATCC 15517 produced 28 to 30 mg of aflatoxin per 100 ml of a medium containing sucrose, asparagine, and salts in stationary and shaken cultures. In the absence of asparagine in the medium, the toxin yields fell drastically, and the thin-layer chromatograms of the chloroform extracts of the cultures indicated the total absence of aflatoxin G, and the presence of new intense blue and green fluorescent bands having RF values lower than aflatoxins. Initial pH was critical and had to be around 4.5 for good growth and high toxin production on this

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Biochemistry and regulation of streptomycin and mannosidostreptomycinase (alpha-D-mannosidase) formation.

Abstract: A new field of research has been opened to us; the possibility of elucidating many complex natural processes heretofore not sufficiently understood and of discovering new agents that may help man in controlling diseases. .. is certainly inviting." Selman A. Waksman, 1943

Cited by 53 publications

References 60 publications

Phosphate inhibition of secondary metabolism in Serratia marcescens

Phosphate inhibition of secondary metabolism in Serratia marcescens

Regulation of Secondary Metabolite Biosynthesis: Catabolite Repression of Phenoxazinone Synthase and Actinomycin Formation by Glucose

High Aflatoxin Production on a Chemically Defined Medium 1

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