Variations in storage protein and carbohydrate levels during development of avocado zygotic embryos

Sánchez-Romero, Carolina; Perán-Quesada, R.; Barceló-Muñóz, Araceli; Pliego‐Alfaro, Fernando

doi:10.1016/s0981-9428(02)01469-9

Cited by 22 publications

(18 citation statements)

References 36 publications

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“…9B, C), corroborating previous histochemical analysis. Similar results were obtained in avocado embryos (Sánchez− Romero et al 2002) and in P. mariana and P. glauca (Iraqui and Tremblay 2001), in which starch accumulation also occurred specifically at final phases of embryonic development.…”

Section: Resultssupporting

confidence: 84%

“…These variations in sucrose, glucose, and fructose levels suggest that they were coordinated during the studied processes and resulted from the metabolism of sucrose, as observed in other species (Hill et al 2003). The decrease in hexoses during development of ZEs of avocado, for example, was attributed to the synthesis of sucrose and coincided with the maturation phase, with a simultaneous decrease in water content and accumulation of starch (Sánchez-Romero et al 2002). The high and practically constant amounts of sucrose in every embryonic stage of A. sellowiana suggest the importance of this sugar to the embryo development, differently, for instance, from P. abies where sucrose acts as a regulatory factor for the maturation of SEs, in addition to signaling the synthesis of reserve proteins (Iraqui and Tremblay 2001).…”

Section: Resultssupporting

confidence: 57%

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Changes in soluble carbohydrates and starch amounts during somatic and zygotic embryogenesis of Acca sellowiana (Myrtaceae)

Pescador

Kerbauy

Kraus

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Comparative analysis of zygotic and somatic embryogenesis of Acca sellowiana showed higher amounts of sucrose, fructose, raffinose, and myo-inositol in zygotic embryos at different developmental stages than in corresponding somatic ones. These differences were mostly constant. In general, glucose levels were significantly lower than the other soluble carbohydrates analyzed, showing minor variation in each embryo stage. Despite the presence of sucrose in the culture medium, its levels conspicuously diminished in somatic embryos compared with the zygotic ones. Raffinose enhanced parallel to embryo development, regardless of its zygotic or somatic origin. Analysis of the soluble carbohydrate composition of mature zygotic cotyledon used as explant pointed out fructose, glucose, myoinositol, sucrose, and raffinose as the most important. Similar composition was also found in the corresponding somatic cotyledon. Total soluble carbohydrates varied inversely, decreasing in zygotic embryos and increasing in somatic embryos until the 24th d, at which time they increased rapidly about sixfold in zygotic embryos until the 27th d, a period coinciding with the zygotic proembryos formation. Such condition seems to reflect directly the variation of endogenous sucrose level, mainly because glucose and fructose diminished continuously during this time period. This means that, in terms of soluble sugars, zygotic embryo formation occurred under a situation represented by high sucrose amounts, simultaneously with low fructose and glucose levels, while in contrast, somatic embryo formation took place under an endogenous sugar status characterized by a substantial fructose enhancement. Starch levels increased continuously in zygotic embryos and decreased in somatic ones, the reverse to what was found in fructose variation. Starch accumulation was significantly higher in somatic torpedo and cotyledonary embryos than in the corresponding zygotic ones.

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Section: Resultssupporting

confidence: 84%

Section: Resultssupporting

confidence: 57%

Changes in soluble carbohydrates and starch amounts during somatic and zygotic embryogenesis of Acca sellowiana (Myrtaceae)

Pescador

Kerbauy

Kraus

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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“…In avocado, too, the total soluble carbohydrate content as well as partitioning between individual sugars has been shown to be influenced by the embryo developmental stages. In addition, a correlation between sucrose and starch levels has been observed (Sanchez-Romero et al 2002). This also seems to be the case in oak ZEs, in which starch accumulation seems to be dependent on a high sucrose concentration in the cotyledon.…”

Section: Discussionmentioning

confidence: 64%

Physiological and morphological characteristics during development of pedunculate oak (Quercus robur L.) zygotic embryos

Prewein

Endemann

Reinöhl

et al. 2005

Trees

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The developmental stages of oak zygotic embryos (ZEs) are characterized here according to morphological and physiological features. Seeds were harvested from June to September in 1-week intervals. Excised embryos were classified into four stages of development by using growth parameters. For physiological characterization, endogenous levels of abscisic acid (ABA), indole-3-acetic acid (IAA), l-proline, starch content and water status were determined. The expression of the oak legumin storage protein gene was tested in immature cotyledonary ZEs before and after ABA treatment. The ABA levels of the embryos showed a significant peak during the intermediate stage of maturation (stage III) and then decreased again at the end of the late maturation phase (stage IV). Concomitant with ABA, the moisture content declined with the maximum embryo size. High IAA levels were found at the beginning of embryo enlargement as exponential growth occurred (stage II) but decreased during further development. Starch accumulated gradually in the course of maturation, whereas significant values were found in stage IV ZEs near shedding. Proline, on fresh weight basis, was high during stages I and II. Osmotic potential increased when, by rapid dry matter accumulation, stage II ZEs reached their maximum size during early intermediate development. Expression of precocious germination was C. Prewein · M. Endemann · V. Sunderlikova · E. Wilhelm ( ) higher on hormone-free medium, in particular, among stage II and stage III ZEs. Variations in phytohormone levels in combination with changes in tissue water status seem to be important factors for oak ZE development.

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“…The latter were then washed twice with their respective buffer to eliminate the unbound protein excess. The captured proteins from each sample were desorbed with 40 μL of an extraction buffer containing 4% ( m / v ) SDS, 12.5% (v/v) glycerol, 2.5% (v/v) 2‐mercaptoethanol, 0.005% ( m / v ) bromophenol blue, and 62.5 mM Tris‐HCl (pH 6.8), for 20 min, under boiling conditions .…”

Section: Methodsmentioning

confidence: 99%

“…The avocado fruit composition has been deeply studied, leading to a good characterization of small size compounds such as fatty acids and sterols . However, up to the present, no work has been focused on identifying the avocado pulp proteome; only some reports have appeared on the avocado seed and root proteins. On the other hand, avocado is a source of allergens that can elicit diverse IgE‐mediated reactions including anaphylaxis in sensitized individuals.…”

Section: Introductionmentioning

confidence: 99%

Identification of avocado (Persea americana) pulp proteins by nano‐LC‐MS/MS via combinatorial peptide ligand libraries

Esteve

D’Amato²,

Marina

et al. 2012

Electrophoresis

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Avocado (Persea americana) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at pH 7.4 and under conditions mimicking reverse-phase capture at pH 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low-abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein, a glucanase, and an isoflavone reductase like protein.

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Variations in storage protein and carbohydrate levels during development of avocado zygotic embryos

Cited by 22 publications

References 36 publications

Changes in soluble carbohydrates and starch amounts during somatic and zygotic embryogenesis of Acca sellowiana (Myrtaceae)

Changes in soluble carbohydrates and starch amounts during somatic and zygotic embryogenesis of Acca sellowiana (Myrtaceae)

Physiological and morphological characteristics during development of pedunculate oak (Quercus robur L.) zygotic embryos

Identification of avocado (Persea americana) pulp proteins by nano‐LC‐MS/MS via combinatorial peptide ligand libraries

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