Variations in glucose metabolism by fat cells from three adipose depots of the rat

Fried, Susan K.; Lavau, M; Pi‐Sunyer, F. Xavier

doi:10.1016/0026-0495(82)90176-7

Cited by 56 publications

(36 citation statements)

References 31 publications

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“…The magnitude of the increase (+ 52 and + 56% in sheep and cow AT, respectively) after 24 h in vitro was similar to that observed by Vernon and Taylor (1988) with ovine subcutaneous AT. Similar results were also noted with isolated rat adipocytes incubated for 2 h (Fried et al, 1982). Moreover, in the present study, insulin produced a more marked stimulation of lactate production on day 2 than on day 1 of incubation, which is in agreement with Plested et al (1987) with sheep subcutaneous AT explants.…”

Section: Overall Analysissupporting

confidence: 92%

“…However, glucose-6-phosphate dehydrogenase activity was similar in cow and sheep subcutaneous AT (Smith and Prior, 1986). In rat AT, 5 to 60% of glucose carbons were converted to lactate depending on the age of the animal (Grandall et al, 1983;Thacker et al, 1987), its nutritional state (Thacker et al, 1987;Newby et al, 1990) and the adipose storage site (Fried et al, 1982;Newby et al, 1988 (Chilliard and Faulconnier, 1995). The magnitude of the increase (+ 52 and + 56% in sheep and cow AT, respectively) after 24 h in vitro was similar to that observed by Vernon and Taylor (1988) with ovine subcutaneous AT.…”

Section: Overall Analysismentioning

confidence: 92%

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Insulin and/or dexamethasone regulation of lactate production and its relationship to glucose utilization by ovine and bovine adipose tissue explants incubated for 7 days

Faulconnier

Ferlay²,

Chilliard³

1997

Reprod. Nutr. Dev.

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Section: Overall Analysissupporting

confidence: 92%

Section: Overall Analysismentioning

confidence: 92%

Insulin and/or dexamethasone regulation of lactate production and its relationship to glucose utilization by ovine and bovine adipose tissue explants incubated for 7 days

Faulconnier

Ferlay²,

Chilliard³

1997

Reprod. Nutr. Dev.

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“…However, few studies have looked at possible differences in insulin action on glucose transport. Regional variation in insulin-stimulated glucose uptake has been reported in freshly isolated adipocytes [14] and newly differentiated cultured fat cells [15] obtained from epididymal, retroperitoneal, and dorsal subcutaneous tissues of rats. However, to the best of our knowledge, direct comparisons of insulin action between subcutaneous and intra-abdominal adipose regions have not yet been reported in either rats or humans.…”

Section: © Springer-verlag 1997mentioning

confidence: 99%

Regional variation in adipose tissue insulin action and GLUT4 glucose transporter expression in severely obese premenopausal women

Marette

Mauriège

Marcotte³

et al. 1997

Diabetologia

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It is now well-documented that insulin resistance is closely associated with the accumulation of fat in the intra-abdominal region [1,2]. Previous studies have shown that whole-body insulin-mediated glucose disposal (measured by the euglycaemic clamp technique) is strongly and negatively correlated with visceral fat accretion, particularly when abdominal adiposity is directly determined by computed tomography or X-ray absorptiometry in both obese and healthy men [3,4], as well as in normal and overweight women [5]. Intra-abdominal obesity is associated with an increased portal non-esterified fatty acid (NEFA) flux which may lead to a reduced hepatic Diabetologia (1997) 40: 590-598 Regional variation in adipose tissue insulin action and GLUT4 glucose transporter expression in severely obese premenopausal women Summary Insulin action and GLUT4 expression were examined in adipose tissue of severely obese premenopausal women undergoing gastrointestinal surgery. Fat samples were taken from three different anatomical regions: the subcutaneous abdominal site, the round ligament (deep abdominal properitoneal fat), and the greater omentum (deep abdominal intraperitoneal fat). The stimulatory effect of insulin on glucose transport and the ability of the hormone to inhibit lipolysis were determined in adipocytes isolated from these three adipose depots. Insulin stimulated glucose transport 2-3 times over basal rates in all adipocytes. However, round ligament adipose cells showed a significantly greater responsiveness to insulin when compared to subcutaneous and omental adipocytes. Round ligament fat cells also displayed the greatest sensitivity and maximal antilipolytic response to insulin. We also investigated whether regional differences in fat cell insulin-stimulated glucose transport were linked to a differential expression of the GLUT4 glucose transporter. GLUT4 protein content in total membranes was 5 and 2.2 times greater in round ligament adipose tissue than in subcutaneous and omental fat depots, respectively. Moreover, GLUT4 mRNA levels were 2.1 and 3 times higher in round ligament than in subcutaneous or omental adipose tissues, respectively. Adipose tissue GLUT4 protein content was strongly and negatively associated (r = -0.79 to -0.89, p < 0.01) with the waist-to-hip ratio but not with total adiposity. In conclusion, these results demonstrate the existence of site differences in adipose tissue insulin action in morbidly obese women. The greater insulin effect on glucose transport in round ligament adipocytes was associated with a higher expression of GLUT4 when compared to subcutaneous abdominal and omental fat cells. Moreover, despite the regional variation in GLUT4 expression, an increased proportion of abdominal fat was found to be associated with lower levels of GLUT4 in all adipose regions investigated. [Diabetologia (1997) 40: 590-598]

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“…We were careful to examine several adipose tissue locations, since it has been clearly established that there are large regional differences in glucose metabolism and its response to insulin in male rats ( 11). As age has also been shown to play a major role in the magnitude of the response to insulin in epididymal adipocytes (12)(13)(14), this comparison was carried out in male and female rats carefully matched for age.…”

Section: Introductionmentioning

confidence: 99%

Increased insulin sensitivity and responsiveness of glucose metabolism in adipocytes from female versus male rats.

Guerre-Millo¹,

Leturque²,

Girard³

et al. 1985

J. Clin. Invest.

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This study was undertaken to examine whether there were sex-associated differences in the action of insulin on glucose metabolism in adipocytes. Insulin binding and the dose-response curves for glucose transport (assessed by measuring the cellassociated radioactivity after 15-s incubation with 50 ;&M 16-'4Clglucose) and [U-'4Cjglucose (5 mM) metabolism into CO2 and lipids were compared in retroperitoneal adipocytes from age-matched (84 d) male and female rats. In addition, the activity of fatty acid synthetase, one of the key lipogenic enzymes, was determined. Fat cell size was not significantly larger in females than in males (0.238 vs. 0.209 jig lipid per cell). At insulin concentrations < 1.6 nM, adipocytes from females bound significantly more insulin than did adipocytes from males, due to an increased apparent affinity of the receptors for insulin. Accordingly, the sensitivity of glucose transport to insulin was greater in females than in males: insulin concentration eliciting half-maximal stimulation (ED50) = 0.19 nM vs. 0.41 nM. At maximal insulin stimulation the rates of glucose transport (12 times the basal values) were similar in the two sexes. In contrast, the maximal effect of insulin on glucose conversion to CO2 plus lipids was much greater in the adipocytes from females than males (increment over basal: 472 vs. 249 nmol/106 cells per 2 h). Fatty acid synthesis contributed -40% of the incremental difference between the two types of adipocytes, while glyceride-glycerol synthesis contributed <10%. The insulin dose-response curves for adipocytes from females were shifted to the left for all the metabolic pathways investigated. The mean ED50 for total glucose metabolism in females was 50% of that in males (0.07 nM vs. 0.15 nM). Marked sex-associated differences in the action of insulin on glucose metabolism were also observed in subcutaneous inguinal adipocytes (increment over basal: 137 and 56 nmol/106 cells per 2 h, ED50 = 0.13 nM and 0.30 nM in females and males, respectively). The intracellular capacity to metabolize glucose through the fatty acid synthesis pathway, as assessed by FAS activity, was higher in adipocytes from females than in those from males and was greater in retroperitoneal than in i guinal adipocytes. Furthermore, by plotting the individual data, a highly significant correlation (r = 0.92, P < 0.001) was found between the absolute effect of insulin on glucose metabolism at maximal stimulation and the fatty acid synthetase activity of the cells.These results indicate that the response of glucose metabolism to insulin in adipocytes from female as compared with male rats is characterized by two main features: (a) an increased sensitivity primarily due to an increase in insulin binding, and (b) an increased responsiveness closely associated with a postreceptor increase in the lipogenic capacity of the cell. These findings might be relevant to the differential disposition of male and female rats to develop fatness.

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Variations in glucose metabolism by fat cells from three adipose depots of the rat

Cited by 56 publications

References 31 publications

Insulin and/or dexamethasone regulation of lactate production and its relationship to glucose utilization by ovine and bovine adipose tissue explants incubated for 7 days

Insulin and/or dexamethasone regulation of lactate production and its relationship to glucose utilization by ovine and bovine adipose tissue explants incubated for 7 days

Regional variation in adipose tissue insulin action and GLUT4 glucose transporter expression in severely obese premenopausal women

Increased insulin sensitivity and responsiveness of glucose metabolism in adipocytes from female versus male rats.

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