Variations in Chromosome Number and Structure in Plant Cells Grown in Suspension Cultures

Kao, K. N.; Miller, Rebecca; Gamborg, O. L.; Harvey, B. L.

doi:10.1139/g70-044

Cited by 76 publications

(21 citation statements)

References 10 publications

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“…The phenomenon of polyploidization in haploid and diploid cells accompanying repetition of subculture has been demonstrated in cultures of several plant species by many workers (Bennici et al 1968;Kao et al 1970;Sacristan 1971;Collins et al 1972;Bayliss 1973Bayliss ,1975bSingh et al 1975;Matthews and Vasil 1976). Demoise and Partanen (1969) demonstrated that with repetition of subculture of calli derived from embryos of Paeonia sufruticosa, the fall in frequency of diploid cells was accompanied by the emergence of tetraploid and octoploid cells.…”

Section: Discussionmentioning

confidence: 97%

Growth characteristics and chromosomal behavior of cell suspension lines established from pollen callus of peony.

Ono

Harashima

1983

The Japanese journal of genetics

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Section: Discussionmentioning

confidence: 97%

Growth characteristics and chromosomal behavior of cell suspension lines established from pollen callus of peony.

Ono

Harashima

1983

The Japanese journal of genetics

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“…Structural changes of chromosomes are often produced in cultured cells : These are dicentric chromosome (Norstog et al 1969;Kao et al 1970 ;Singh et al 1972 ;Bayliss 1975;Orton 1980), acentric fragment (Norstog et al 1969 ;Kao et al 1970;Singh et al 1972), translocation (Sacristan andWendt-Gallitelli 1971, 1973), and deletion of a chromosome segment (the present work). These changes reported in cultured cells were reviewed by Sunderland (1977).…”

Section: Discussionmentioning

confidence: 79%

Tissue culture in Haworthia. V. Characterization of chromosomal changes in cultured callus cells.

Ogihara

1982

The Japanese journal of genetics

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The chromosomal variations occurring during the subculture of Haworthia setata (2n =14) calluses that had originated from a single callus induced from a flower bud, repeatedly subdivided in the first four culture generations, and successively subcultured for six more generations were examined. The frequency of diploid cells remained constant during subculture (ca. 50%), but that of the tetraploids gradually increased. Both hyperdiploid and hypo-or hypertetraploid cells remained as minor fractions. But, the frequency of hypodiploid cells was changeable.Though chromosome number differed significantly among some culture generations, its variation did not increase with advanced culture generations.A new variant karyotype, 7L+1M+6S, was first observed in the third culture generation, and came to constitute the modal class in three of the four primary clones, replacing the normal diploid karyotype (8L +6S). A selecting force to maintain the euploids, including the 7L + 1M + 6S type, was operating during callus growth. Structural changes of chromosomes found in the Haworthia callus were similar to those reported in other materials, i.e., deletions, translocations, dicentric and acentric fragments.All of the chromosomes were broken in the interstitial region, and not in the centromeric region.

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“…The :3',. with 14 chromosomes leading to their predominance in the population, as suggested by Kao et al, (1970). The two tetraploid lines also differed in stability.…”

Section: Discussionmentioning

confidence: 98%

A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats

1993

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Chromosome instability was monitored' through time in cell suspensions of the diploids Triticum tauschii (the D genome donor) and T monococcum (the A genome donor), the tetraploids, T durum and T dicoccum (both AABB) and in the cultivars Copain and Sicco of hexaploid (AABBDD) breadwheat (T aestivum). Differences in stability were observed between the different ploidy levels and between species within the ploidy levels. The diploids were most stable, particularly T. tauschii, and the hexaploids were least stable. Instability was greatest in all the lines during the first months of culture. In all but one line, very few structural aberrations were observed, even after many months in culture. Morphogenetic capacity was lost in all lines, even though some retained high proportions of euploid cells. The relevance of these results is discussed in relation to the regeneration of breadwheat from protoplasts.

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Variations in Chromosome Number and Structure in Plant Cells Grown in Suspension Cultures

Cited by 76 publications

References 10 publications

Growth characteristics and chromosomal behavior of cell suspension lines established from pollen callus of peony.

Growth characteristics and chromosomal behavior of cell suspension lines established from pollen callus of peony.

Tissue culture in Haworthia. V. Characterization of chromosomal changes in cultured callus cells.

A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats

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