A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats

Winfield, Mark; Davey, M. R.; Karp, A.

doi:10.1038/hdy.1993.28

Cited by 22 publications

(8 citation statements)

References 24 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a comparison of cell suspensions of diploid, tetraploid and hexaploid wheats, the diploids were found to be the most stable and the hexaploids the least stable, but genotype differences were evident within the ploidy levels (Winfield et al ., 1993) . Similarly, several reports indicate that somaclonal variation is minimal in regenerants of barley Karp et al, 1987 ;Ruiz et al ., 1992), but quite extensive in another diploid cereal, rye Linacero & Vazquez, 1992) .…”

Section: Introductionmentioning

confidence: 99%

Somaclonal variation as a tool for crop improvement

Karp

1995

Euphytica

Self Cite

171

View full text Add to dashboard Cite

Somaclonal variation is a tool that can be used by plant breeders . The review examines where this tool can be applied most effectively and the factors that limit or improve its chances of success . The main factors that influence the variation generated from tissue culture are (1) the degree of departure from organised growth, (2) the genotype, (3) growth regulators and (4) tissue source. Despite an increasing understanding of how these factors work it is still not possible to predict the outcome of a somaclonal breeding programme . New varieties have been produced by somaclonal variation, but in a large number of cases improved variants have not been selected because (1) the variation was all negative, (2) positive changes were also altered in negative ways, (3) the changes were not novel, or (4) the changes were not stable after selfing or crossing . Somaclonal variation is cheaper than other methods of genetic manipulation. At the present time, it is also more universally applicable and does not require `containment' procedures . It has been most successful in crops with limited genetic systems and/or narrow genetic bases, where it can provide a rapid source of variability for crop improvement .

show abstract

Section: Introductionmentioning

confidence: 99%

Somaclonal variation as a tool for crop improvement

Karp

1995

Euphytica

Self Cite

171

View full text Add to dashboard Cite

show abstract

“…The assessment of ploidy status performed by the aid of flow cytometry proved to be an efficient tool to detect the ploidy level of a plant, as reported (Dickson et al, 1992;Gantait et al, 2011a). It was reported that in comparison to the other higher ploidy levels (tetraploid or hexaploid) the diploids were found to be the most stable (Winfield et al, 1993). Yet, there are several reports on the extensive alteration of ploidy level in a number of diploid species (Karp et al, 1992;Linacero and Vazquez, 1992).…”

Section: Analysis By Flow Cytometrymentioning

confidence: 90%

Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants

Gantait¹,

Uma²

2011

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Micropropagated and seed propagated plantlets of anthurium (Anthurium andreanum Lind. cv. CanCan) were transferred to similar field condition and growth stage. A comparative study was conducted using morphological parameters, ploidy level assessment as well as deoxyribonucleic acid (DNA) fingerprinting using inter simple sequence repeat (ISSR) markers. The in vitro generated anthurium plants exhibited comparable vegetative growth and more sucker production when compared to plants propagated through seeds. No variation in ploidy level was established through flow cytometric study. Genetic assessment through ISSR showed no polymorphism in banding pattern. It was revealed that there was no significant variation between micropropagated and seed propagated plants at ploidy and molecular level assuring the trueness of the micropropagated anthurium clones and their commercial applicability.

show abstract

“…Pretreatment and fixation of root tips and suspensioncultured cells for chromosome analyses were performed according to Winfield et al (1993). A minimum of 5 slides and 50 complete cells were examined per sample.…”

Section: Cytological Analysismentioning

confidence: 99%

“…Creso, 2n = 28; 7: aestivum cv. Sicco, 2n = 42) indicated that instability was related to the allohexaploid constitution of wheat (Bang 1990;Winfield et al 1993), with diploids being more stable than hexaploids.…”

Section: Introductionmentioning

confidence: 96%

Nonrandom chromosome variation and morphogenic potential in cell lines of bread wheat (Triticum aestivum L.)

Winfield¹,

Karp²,

Schmitt³

et al. 1995

Genome

View full text Add to dashboard Cite

A cytogenetical analysis of 18 cell lines, 9 microspore derived, 6 anther derived, and 3 immature-embryo derived, of bread wheat (Triticum aestivum L.) varying in their morphogenic potential was undertaken. Chromosome variation, both structural and numerical, was detected in all lines studied. Variation was present and, in some cases quite extensive, in the earliest samples taken (only 12 weeks after initiation of the suspensions). Within any culture, the pattern and extent of variation changed throughout the course of the study and cells with a euploid constitution generally decreased in frequency with culture age. Among the nine microspore-derived suspensions, morphogenic lines generally showed a more restricted range of chromosome numbers and higher proportions of euploid cells than nonmorphogenic lines. The patterns of distribution of chromosome numbers among the anther-derived cultures were similar to those of the microspore-derived lines but the correspondence between instability and regenerative capacity was less. The immature embryo derived lines, which were neither regenerable nor morphogenic, were all unstable. The anther-derived lines were sampled over several months to determine whether loss of morphogenic potential was related to changes in chromosome instability of specific lines. Analysis of the "elite" line Fl.7, initially capable of regenerating green plants, showed that substantial decreases in the frequencies of normal euploid cells (from 45 to 5%) occurred over the period when morphogenic capacity was lost. However, whether the chromosome instability resulted in loss of morphogenicity or vice versa was not clarified. C-banding analyses of lines Fl.7 and C82d indicated that instability was not random with respect to the three genomes (A, B, and D) of wheat nor to the different chromosomes within the genomes. Chromosomes of the B genome were most often lost or involved in rearrangements, with breakpoints located at, or near, the heterochromatic blocks. Because of the heterogeneity of the cell lines, extensive analyses of large numbers of cells would be required before it would be possible to determine whether loss of morphogenic potential arises as a result of specific chromosome loss(es).

show abstract

A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats

Cited by 22 publications

References 24 publications

Somaclonal variation as a tool for crop improvement

Somaclonal variation as a tool for crop improvement

Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants

Nonrandom chromosome variation and morphogenic potential in cell lines of bread wheat (Triticum aestivum L.)

Contact Info

Product

Resources

About