Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants

Gantait, Saikat; Uma, Rani Sinniah

doi:10.5897/ajb11.1855

Cited by 17 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ISSR approach has been widely used to analyze the genetic stability of vitroplants as well as for varietal characterization in date palm (Zehdi et al 2002). In other species, ISSR markers have been successfully used to detect genetic differences or similarities in micropropagated plants including gerbera (Bhatia et al 2009), anthurium (Gantait and Sinniah 2011), and grape (Nookaraju and Agrawal 2012).…”

Section: Discussionmentioning

confidence: 99%

2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

Baklouti¹,

Beulé²,

Nasri³

et al. 2021

Preprint

View full text Add to dashboard Cite

The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L− 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g− 1 to 2973 nmol g− 1 FW when 40 mg L− 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA); likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8–18.93%.

show abstract

Section: Discussionmentioning

confidence: 99%

2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

Baklouti¹,

Beulé²,

Nasri³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

Baklouti

Beulé

Nasri

et al. 2022

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L − 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some signi cant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g − 1 to 2973 nmol g − 1 FW when 40 mg L − 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA); likewise, the speci c activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also con rmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8-18.93%. Key MessageResults demonstrate that the 2,4-D can affect physiological and molecular parameters of vitrocultures when used at high concentrations without hampering their morphogenetic capacities. It was found to be e cient at inducing genetic and epigenetic variations.

show abstract

Plant regeneration from root segments of Anthurium andraeanum and assessment of genetic fidelity of in vitro regenerates

Wang

Qiao

Chao

2021

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants

Cited by 17 publications

References 26 publications

2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

Plant regeneration from root segments of Anthurium andraeanum and assessment of genetic fidelity of in vitro regenerates

Contact Info

Product

Resources

About