Variations in Antioxidant Genes and Male Infertility

Yu, Bolan; Huang, Zhaofeng

doi:10.1155/2015/513196

Cited by 62 publications

(37 citation statements)

References 102 publications

(105 reference statements)

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“…Most of these components have already been shown to regulate sperm function, as reviewed in mammals (Dun et al, 2012; Yu & Huang, 2015) and avian species (Breque, Surai, & Brillard, 2003; Hiyama et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Riou

Brionne

Cordeiro

et al. 2020

Molecular Reproduction Devel

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Uterine fluid is an aqueous milieu to which sperm are exposed during their storage and ascent. In this study, a bottom-up proteomic strategy and bioinformatic analysis of hen uterine fluid was performed to improve the understanding of this fluid and its potential role in sperm survival mechanisms. The proteomic data were submitted to ProteomeXchange. Among the 913 proteins identified, 160 are known to be secreted and 640 are referenced in exosomes databases. We isolated exosomes from the avian uterine fluid, analyzed them using electron microscopy, and targeted several exosomes markers (ANXA1/2/4/5, VCP, HSP90A, HSPA8, PARK7, and MDH1) using immunoblotting. Electron microscopy and immunohistochemistry were also used to analyze uterovaginal junctions for the exosomal proteins ANXA4, VCP, and PARK7. Exosomes were observed both at the surface epithelium and inside sperm storage tubules. Our data were compared with two previously published studies on proteomic of hen uterine fluid, and with one study describing the proteomic content of rooster seminal plasma and sperm. In conclusion, we demonstrated for the first time that avian uterine fluid contains exosomes. These may play a key role in preserving sperm functions within the female genital tract. Their presence in the sperm storage tubules may represent an important mechanism regarding interaction between the female genital tract and sperm. K E Y W O R D S avian, exosomes, proteome, sperm, uterine fluid

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Section: Discussionmentioning

confidence: 99%

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Riou

Brionne

Cordeiro

et al. 2020

Molecular Reproduction Devel

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show abstract

“…Superoxide dismutase ( SOD ), nitric oxide synthase ( NOS ), glutathione peroxidase ( GPX ), glutathione S‐transferase ( GST ), nuclear factor erythroid 2‐related factor 2 ( NRF2 ) and catalase ( CAT ) are several antioxidant genes that have been found to be associated with male infertility. The risk of male infertility might be correlated with polymorphisms or genetic variations in these genes via decreasing sperm quality (Yu & Huang, ). A case–control study including 314 heavy smokers and 314 matched nonsmoker controls with idiopathic infertility investigated the association among polymorphisms of NRF2 gene, mRNA expression levels of GSTM1, SOD2 , NRF2 and CAT antioxidant genes, and the level of seminal SOD activity.…”

Section: Cigarette Smoking and Genetic Alterationsmentioning

confidence: 99%

Smoking-induced genetic and epigenetic alterations in infertile men

et al. 2018

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Male fertility rates have shown a progressive decrease in both developing and industrialised countries in the past 50 years. Clinical and epidemiological studies have demonstrated controversial results about the harmful effects of cigarette smoking on seminal parameters. Some studies could not establish a negative effect by tobacco smoking on sperm quality and function, whereas others have found a significant reduction in sperm quality and function. This study reviews the components in cigarette smoke and discusses the effects of smoking on male fertility by focusing extensively on smoking-induced genetic and epigenetic alterations in infertile men. Chromosomal aneuploidies, sperm DNA fragmentation and gene mutations are discussed in the first section, while changes in DNA methylation, chromatin remodelling and noncoding RNAs are discussed in the second section as part of epigenetic alterations.

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“…Smoking switch off this important gene, but the mechanism is not well known. It is worth to say that smoking could work on the susceptible patient having NRF2 single nucleotide polymorphism [21] . It is expected that genetic variations in major antioxidant genes will alter the susceptibility of a male to infertility and defective spermatogenesis [22] .…”

Section: Discussionmentioning

confidence: 99%

NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram

et al. 2017

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Introduction: Male factor is responsible for about half of infertility problems. However, the reasons for the decrease in male fertility are still broadly unclear. The mechanisms of how smoking may impact male fertility have not been established. However, with its influence on different semen parameters, it is regarded as a risk factor for infertility. Aim: To investigate the effect of chronic smoking on spermatozoa NRF2 expression and DNA fragmentation in infertile men with apparently normal seminogram and to determine if NRF2 expression and DNA fragmentation markers could be possible predictors of the impact of chronic cigarette smoking on male fertility. Methods: Semen samples were collected from 170 subjects; 65 nonsmokers (40 fertile and 25 infertile) and 105 smokers (25 fertile and 80 infertile). NRF2 gene expression, 8-OHdG and DNA fragmentation were assayed. Results: There were significant increases in 8-OHdG and %DNA fragmentation with a significant decrease in NRF2 gene expression in infertile smokers. ROC curve analysis of spermatozoa NRF2 gene expression showed 95% sensitivity 93.3% specificity at cutoff value ≤0. 931 (p <0.0001) differentiating infertile smokers from controls. Moreover, seminal 8-OHdG assay shows 93.4% sensitivity, 89.2% % specificity, at cutoff values >19.33 pg/ml predicting the detrimental effect of smoking on spermatozoa DNA. Conclusion: Chronic cigarette smoking may be a hidden causative mechanism of delayed fertility. Spermatozoa NRF2 gene expression and seminal 8-OHdG levels may serve as sensitive diagnostic indicators predicting smoking induced infertility. So, the presence of normal seminal parameters could not be an exclusion of potential effect of chronic smoking on male fertility.

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Variations in Antioxidant Genes and Male Infertility

Cited by 62 publications

References 102 publications

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival

Smoking-induced genetic and epigenetic alterations in infertile men

NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram

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