NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram

Elsamanoudy, Ayman Z.; Shaalan, Dalia; El-Khair, Salwa M Abo; Gaballah, Mohammad A.; State, Ahmed Fathy; Helaly, Ahmed M.N.

doi:10.21608/ha.2017.1628.1014

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…Another lifestyle factor that causes an increase in ROS generation leading to infertility is heavy alcohol consumption (Das & Vasudevan, 2007; Jensen, Gottschau, et al, 2014; Jensen, Swan, et al, 2014). In addition, alcohol intake and smoking affect and repress Nrf2 expression, which plays a major role in protection against oxidative stress (Elsamanoudy et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Impact of heavy alcohol consumption and cigarette smoking on sperm DNA integrity

et al. 2022

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The purposes of the presents study were to investigate the impact of alcohol consumption and cigarette smoking on semen parameters and sperm DNA quality, as well as to determine whether tobacco smoking, or alcohol consumption causes more deterioration of sperm quality. Two hundred and eleven semen samples of men were included in this study. Four groups were studied: heavy smokers (N = 48), heavy drinkers (N = 52), non‐smokers (n = 70), and non‐drinkers (n = 41). Semen parameters were determined according to WHO guidelines, protamine deficiency assessed by chromomycin (CMA3) staining, and sperm DNA fragmentation (sDF) evaluated by TUNEL assay. Sperm parameters were significantly higher in non‐smokers versus smokers and in non‐drinkers versus drinkers (p < 0.005). However, protamine deficiency and sDF were significantly lower in non‐smokers versus smokers and in non‐drinkers versus drinkers (p < 0.0001). No significant difference in the semen analysis parameters was observed between heavy smokers and heavy drinkers (semen volume: 3.20 ± 1.43 vs. 2.81 ± 1.56 ml, semen count: 65.75 ± 31.32 vs. 53.51 ± 32.67 mill/ml, total motility: 24.27 ± 8.18 vs. 23.75 ± 1.75%, sperm vitality: 36.15 ± 18.57 vs. 34.62 ± 16.65%, functional integrity: 41.56 ± 18.57 vs. 45.96 ± 17.98% and the morphologically normal spermatozoa: 28.77 ± 11.82 vs. 27.06 ± 13.13%, respectively). However, protamine deficiency was significantly higher among drinkers than smokers (37.03 ± 9.75 vs. 33.27 ± 8.56%, p = 0.020). The sDF was also significantly higher among drinkers than smokers (22.37 ± 7.60 vs. 15.55 ± 3.33%, p < 0.0001). Thus, cigarette smoking, and heavy alcohol intake can deteriorate sperm quality. However, alcohol consumption deteriorates sperm maturity and damages DNA integrity at significantly higher rates than cigarette smoking.

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Section: Discussionmentioning

confidence: 99%

Impact of heavy alcohol consumption and cigarette smoking on sperm DNA integrity

et al. 2022

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“…On the other hand, significant enhancement in the SDF in the diabetic group might be caused by insufficient expression of seminal antioxidant enzymes regulated by Nrf2 gene expression and therefore failure in the protection against DNA damage. Indeed, oxidative stress caused by diabetics might effect on the spermatozoa functions as a hidden causative infertility agent which might be occurred through the increased SDF secondary to spermatozoa oxidative stress as a result of preventing spermatozoa Nrf2 gene expression (Elsamanoudy, Shaalan, El‐khair, Salwa, & Helaly, ).…”

Section: Discussionmentioning

confidence: 99%

Amelioration of diabetes-induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment

et al. 2018

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Cerium oxide nanoparticles (CNPs) as an antioxidant have been used frequently to attenuate hyperglycaemia oxidative damage in different organs. We investigated the impact CNPs on the qualitative and quantitative sperm parameters, spermatogenesis and NFE2-related factor 2 (Nrf2) expression as a major contributor of antioxidant defence in the male diabetic rats. Twenty-four male rats were divided into four groups. Controls received only mouse food and water. Second group were treated with CNPs (30 mg kg day ) for 2 weeks. Rats in third group received streptozotocin (STZ) (60 mg/kg). In fourth group, animals became diabetic and received CNPs (30 mg kg day ) for 2 weeks. The results showed a significant abnormality in the sperm parameters and histopathological patterns of testes in the diabetic group compared to the control group and CNPs treatment significantly improved all testicular parameters. Following CNPs administration, sperm DNA fragmentation significantly reduced in the STZ-treated rats. Moreover, after CNPs intake in the STZ-treated rats, Nfr2 expression levels increased significantly. Overall, CNPs administration on the diabetic rates can attenuate detrimental effects of diabetes on the sperm potential fertility, sperm parameters, DNA integrity and Nrf2 expression levels. This study gives a future prospect to determine the role of CNPs in the context of diabetes.

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“…Furthermore, GPx and GSH reductase might be responsible for the inhibition of sperm peroxidation [92] . Moreover, smoking and alcohol drinking also suppress Nrf2 expression, which has a protective role against oxidative damage [93] ( fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Impact of Smoking and Alcohol Consumption on Oxidative Status in Male Infertility and Sperm Quality

Ramgir¹,

Abilash²

2019

ijps

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Ramgir and Abilash: Smoking and Alcohol in Male Infertility The most common unhealthy lifestyle factors like cigarette smoking and drinking alcohol have controversial reports on how these impact male fertility. The present study assessed the potential effects of these habits on sperm quality, male reproductive hormones, and blood plasma oxidative profile in 133 infertile men with 124 fertile, healthy controls. Among the participants, 28 (10.8 %), 29 (11.2 %), 33 (12.8 %), 34 (13.2 %) fertile men were non-alcoholics and non-smokers (total abstainers), smokers, alcohol drinkers, and smokers plus alcohol drinkers, respectively. On the other hand, 32 (12.4 %), 30 (11.6 %), 34 (13.2 %), 37 (14.3 %) infertile men were total abstainers, smokers, alcohol drinkers, and smokers plus alcohol drinkers, respectively. The conventional sperm parameters and reproductive hormone levels were found to be significantly different in the subsets of infertile men than those of fertile men (p<0.001). In these subsets of infertile men, blood plasma malondialdehyde, nitric oxide concentrations were significantly higher and superoxide dismutase, reduced glutathione activities were significantly lower when compared to fertile men and infertile men total abstainers group (p<0.001). In infertile subjects, particularly smoking-alcohol drinking group was more affected than only smoking or alcohol drinking groups. Furthermore, these oxidative markers were correlated with sperm concentration, motility, and morphology, except nitric oxide concentration, which was only associated with sperm motility. These findings highlighted that blood plasma antioxidants are as efficient as seminal plasma antioxidants in male fertility evaluation.

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NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram

Cited by 4 publications

References 17 publications

Impact of heavy alcohol consumption and cigarette smoking on sperm DNA integrity

Impact of heavy alcohol consumption and cigarette smoking on sperm DNA integrity

Amelioration of diabetes-induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment

Impact of Smoking and Alcohol Consumption on Oxidative Status in Male Infertility and Sperm Quality

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