Variation of prostaglandin E2 concentrations in ovaries and its effects on ovarian maturation and oocyte proliferation in the giant fresh water prawn, Macrobrachium rosenbergii

Role of Arachidonic Acid and COX Inhibitors in the Regulation of Reproduction in Freshwater Crab Oziothelphusa senex senex

“…Similar to mammals, NSAIDs affect the synthesis of PGs in invertebrates NSAIDs blocks the COX pathway [18,45], thereby the physiological roles of PGs in the present study the injection of COX inhibitors, indomethacin and aspirin individually and co-administration with AA resulted in a significant decrease in ovarian index, oocyte diameter and ovarian vitellogenin levels. There are no direct studies available to support the present results, but previous studies supported the COX-inhibiting role of non-steroidal anti-inflammatory drugs in crustaceans [21][22][23][24][25]19,46]. The exposure to IBU reduces the reproductive performance in Daphina magna and Moina macrocopa [24].…”

“…Further, this was supported by several studies in different crustaceans where PGs, stimulates ovarian maturation in freshwater prawn Metapenaeus affinis [16], in crayfish [17], in freshwater crab, Oziothelphusa senex senex [13], in the shrimp Penaeus monodon [11] and in the freshwater giant prawn Macrobrachium rosenbergii [19]. Recently, it was demonstrated that administration of PGE 2 increases the vitellogenin levels in hemolymph of Macrobrachium rosenbergii [19]. Similar to earlier studies, in the present study, the regulation of reproduction in crab, Oziothelphusa senex senex by AA may connect to its role as a precursor to prostaglandins.…”

“…This study also reported that injection of PGE 2 and PGF 2α increased ovarian index and oocyte diameter in a dose-dependent manner and no changes were observed on ovarian growth with an injection of PGD 2 in Oziothelphusa senex senex. Recently, the variation of PGE 2 levels at different vitellogenesis stages were demonstrated in freshwater prawn, Macrobrachium rosenbergii and administration of PGE 2 stimulated the ovarian maturation, increasing ovarian somatic index, oocyte proliferation and vitellogenin (Vg) level in the hemolymph of prawn [19]. In contrast to these studies, it was reported that injection of PGE 2 0.1 μg/g body weight did not stimulate the ovarian maturation in Penaeus esculentus [20].…”

Effects of arachidonic acid supplementation in maturation diet on female reproductive performance and larval quality of giant river prawn (Macrobrachium rosenbergii)

“…The roles of PGE2 have been more extensively examined and demonstrated in several shrimp species, including M. rosenbergii . PGE2 is associated with oocyte maturation (e.g., P. semisulcatus; Ravid et al, 1999; M. rosenbergii , Sumpownon et al, 2015), vitellogenesis (e.g., P.japonicus; Tahara & Yano, 2004; M. rosenbergii , Sumpownon et al, 2015) and spawning ( M. rosenbergii, Spaziani, Hinsch & Edwards, 1993; Spaziani, Hinsch & Edwards, 1995). PGF2 α , on the other hand, has been recognized for its importance in ovarian maturation in a few crustaceans, including a crayfish Procambarus paeninsulanus , and Panaeid marine shrimp ( P. japonicus , Tahara & Yano, 2004; P. monodon , Wimuttisuk et al, 2013).…”

“…In M. rosenbergii , the biosynthesis of PGE2 occurs in the ovary and the PGE2 levels are high during early ovarian stages compared to more mature ovaries (Sumpownon et al, 2015). PGE2 and key relevant enzymes, namely cycloxygenase1 and PGE synthase, were present in cytoplasm of previtellogenic oocytes.…”