Variation of mechanical behavior of β-TCP/collagen two phase composite scaffold with mesenchymal stem cell in vitro

Arahira, Takaaki; Todo, Mitsugu

doi:10.1016/j.jmbbm.2016.04.019

Cited by 21 publications

(8 citation statements)

References 28 publications

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“…Most of the 3D scaffolds for bone healing have limited tissue ingrowth due to restrained nutrient supply imposed by intrinsic geometrical and structural characteristics [37]. β-TCP has been used to fabricate scaffolds owing to its good osteoconductivity and degradation [38, 39]. The β-TCP scaffold with interconnected pores was sequentially wrapped by the osteogenic cell sheet and prevascularized cell sheet layer by layer to fabricate engineered periosteum, which enlarged the area of cell adhesion, proliferation, and tissue ingrowth, as well as the subsequent ECM formation.…”

Section: Discussionmentioning

confidence: 99%

Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

Zhang

Gao

et al. 2017

Stem Cell Res Ther

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BackgroundThere is a critical need for the management of large bone defects. The purpose of this study was to engineer a biomimetic periosteum and to combine this with a macroporous β-tricalcium phosphate (β-TCP) scaffold for bone tissue regeneration.MethodsRat bone marrow-derived mesenchymal stem cells (rBMSCs) were harvested and cultured in different culture media to form undifferentiated rBMSC sheets (undifferentiated medium (UM)) and osteogenic cell sheets (osteogenic medium (OM)). Simultaneously, rBMSCs were differentiated to induced endothelial-like cells (iECs), and the iECs were further cultured on a UM to form a vascularized cell sheet. At the same time, flow cytometry was used to detect the conversion rates of rBMSCs to iECs. The pre-vascularized cell sheet (iECs/UM) and the osteogenic cell sheet (OM) were stacked together to form a biomimetic periosteum with two distinct layers, which mimicked the fibrous layer and cambium layer of native periosteum. The biomimetic periostea were wrapped onto porous β-TCP scaffolds (BP/β-TCP) and implanted in the calvarial bone defects of rats. As controls, autologous periostea with β-TCP (AP/β-TCP) and β-TCP alone were implanted in the calvarial defects of rats, with a no implantation group as another control. At 2, 4, and 8 weeks post-surgery, implants were retrieved and X-ray, microcomputed tomography (micro-CT), histology, and immunohistochemistry staining analyses were performed.ResultsFlow cytometry results showed that rBMSCs were partially differentiated into iECs with a 35.1% conversion rate in terms of CD31. There were still 20.97% rBMSCs expressing CD90. Scanning electron microscopy (SEM) results indicated that cells from the wrapped cell sheet on the β-TCP scaffold apparently migrated into the pores of the β-TCP scaffold. The histology and immunohistochemistry staining results from in vivo implantation indicated that the BP/β-TCP and AP/β-TCP groups promoted the formation of blood vessels and new bone tissues in the bone defects more than the other two control groups. In addition, micro-CT showed that more new bone tissue formed in the BP/β-TCP and AP/β-TCP groups than the other groups.ConclusionsInducing rBMSCs to iECs could be a good strategy to obtain an endothelial cell source for prevascularization. Our findings indicate that the biomimetic periosteum with porous β-TCP scaffold has a similar ability to promote osteogenesis and angiogenesis in vivo compared to the autologous periosteum. This function could result from the double layers of biomimetic periosteum. The prevascularized cell sheet served a mimetic fibrous layer and the osteogenic cell sheet served a cambium layer of native periosteum. The biomimetic periosteum with a porous ceramic scaffold provides a new promising method for bone healing.

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Section: Discussionmentioning

confidence: 99%

Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

Zhang

Gao

et al. 2017

Stem Cell Res Ther

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“…Some scholars have prepared β-TCP based scaffolds for bone regeneration [60] . Compared with HA, β-TCP has a faster degradation rate and can be completely replaced by newly formed bone tissues [61] .…”

Section: Collagen Based Composite Scaffolds For Bone Regenerationmentioning

confidence: 99%

The development of collagen based composite scaffolds for bone regeneration

Zhang

Chen

et al. 2018

Bioactive Materials

347

215

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Bone is consisted of bone matrix, cells and bioactive factors, and bone matrix is the combination of inorganic minerals and organic polymers. Type I collagen fibril made of five triple-helical collagen chains is the main organic polymer in bone matrix. It plays an important role in the bone formation and remodeling process. Moreover, collagen is one of the most commonly used scaffold materials for bone tissue engineering due to its excellent biocompatibility and biodegradability. However, the low mechanical strength and osteoinductivity of collagen limit its wider applications in bone regeneration field. By incorporating different biomaterials, the properties such as porosity, structural stability, osteoinductivity, osteogenicity of collagen matrixes can be largely improved. This review summarizes and categorizes different kinds of biomaterials including bioceramic, carbon and polymer materials used as components to fabricate collagen based composite scaffolds for bone regeneration. Moreover, the possible directions of future research and development in this field are also proposed.

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“…Significant progress has been made recently in bone tissue engineering to provide higher regeneration rates with ideal mechanical properties suitable for bone tissues [1,2]. Scaffolds are necessary to support the damaged tissues, allowing the cells to proliferate, differentiate, and gradually replaced by new bone tissue [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Nanocomposite Scaffolds (Collagen/β-TCP/SrO) for Bone Tissue Engineering

Goodarzi

Hashemi‐Najafabadi

Baheiraei

et al. 2019

Tissue Eng Regen Med

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BACKGROUND: Nowadays, production of nanocomposite scaffolds based on natural biopolymer, bioceramic, and metal ions is a growing field of research due to the potential for bone tissue engineering applications. METHODS: In this study, a nanocomposite scaffold for bone tissue engineering was successfully prepared using collagen (COL), beta-tricalcium phosphate (b-TCP) and strontium oxide (SrO). A composition of b-TCP (4.9 g) was prepared by doping with SrO (0.05 g). Biocompatible porous nanocomposite scaffolds were prepared by freeze-drying in different formulations [COL, COL/b-TCP (1:2 w/w), and COL/b-TCP-Sr (1:2 w/w)] to be used as a provisional matrix or scaffold for bone tissue engineering. The nanoparticles were characterized by X-ray diffraction, Fourier transforms infrared spectroscopy and energy dispersive spectroscopy. Moreover, the prepared scaffolds were characterized by physicochemical properties, such as porosity, swelling ratio, biodegradation, mechanical properties, and biomineralization. RESULTS: All the scaffolds had a microporous structure with high porosity (* 95-99%) and appropriate pore size (100-200 lm). COL/b-TCP-Sr scaffolds had the compressive modulus (213.44 ± 0.47 kPa) higher than that of COL/b-TCP (33.14 ± 1.77 kPa). In vitro cytocompatibility, cell attachment and alkaline phosphatase (ALP) activity studies performed using rat bone marrow mesenchymal stem cells. Addition of b-TCP-Sr to collagen scaffolds increased ALP activity by 1.33-1.79 and 2.92-4.57 folds after 7 and 14 days of culture, respectively. CONCLUSION: In summary, it was found that the incorporation of Sr into the collagen-b-TCP scaffolds has a great potential for bone tissue engineering applications.

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Variation of mechanical behavior of β-TCP/collagen two phase composite scaffold with mesenchymal stem cell in vitro

Cited by 21 publications

References 28 publications

Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

The development of collagen based composite scaffolds for bone regeneration

Preparation and Characterization of Nanocomposite Scaffolds (Collagen/β-TCP/SrO) for Bone Tissue Engineering

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