Variation of aluminium tolerance in lentil (Lens culinaris Medik.)

Abstract

With 5 figures and 5 tables Abstract Aluminium (Al) tolerance of 60 genotypes was evaluated using the growth response and haematoxylin staining methods. Major traits used to evaluate Al tolerance were score of root staining, root regrowth after staining, root and shoot length, dry weight of roots and shoots, and pods/plant. Significant variability was noted for tolerance to Al toxicity among the lentil genotypes for all traits except score of root staining. Score of haematoxylin root staining did not show any correlation with growth response method. However, root regrowth after staining showed significant correlation with root and shoot length, dry weight of roots and shoots, and pods/plant. In a further long‐term experiment, the Al tolerance of three tolerant (‘L‐7903’, ‘L‐4602’ and ‘ILL‐6002’) and two sensitive (‘L‐4147’ and ‘BARI Massor‐4’) lines was assessed at the adult stage to evaluate the consistency of Al tolerance. This study also showed that Al tolerance of lentil does not vary with plant growth stage, so selection for increased Al tolerance can be carried out at the seedling growth stage.

Aluminium tolerance in lentil (Lens culinaris Medik.) with monogenic inheritance pattern

“…Root regrowth is one of the most indicative traits, as it allows preliminary screening of a large number of genotypes under hydroponic condition (Singh et al. ) and is mostly used in genetic studies (Khatiwada et al. , Singh and Choudhary ).…”

“…The parental lines ‘L‐7903’, ‘L‐4602, ‘BM‐4’ and ‘L‐4147’ were selected at seedling and adult stages on the basis of previous inferences on the assessment of aluminium tolerance (Singh et al. , ). The experiments were carried out at National Phytotron Facility, Indian Agricultural Research Institute, New Delhi, India.…”

“…Mean primary root regrowth <0.5 cm was classified as Al‐sensitive, while those with the mean root regrowth significantly >1.0 cm were considered tolerant (Singh et al. ).…”

“…The most reliable screening parameters are root regrowth after staining (Singh et al. ) and callose accumulation (Wissemeier et al. , Zhang et al.…”

“…Genetic variation for Al 3+ tolerance exists among lentil genotypes (Singh et al. ). Several studies have demonstrated that Al tolerance is a simple (Rhue et al.…”