Aluminium tolerance in lentil (Lens culinaris Medik.) with monogenic inheritance pattern

Abstract

The objectives of this study were to determine genetics of Al tolerance and whether the Al tolerance observed is governed by the same gene. The lines 'L-7903' and 'L-4602' have been developed through breeding programme as Al-tolerant lines. These lines showed maximum root regrowth and minimum accumulation of Al and callose as compared to sensitive genotypes ('BM-4' and 'L-4147'). Al tolerance in the parents, F 1 , F 2 and backcross generations was estimated using the regrowth of the primary root after staining and scoring of fluorescent signals. The F 1 hybrids responded similarly to the tolerant parents, indicating dominance of Al tolerance over sensitivity. The segregation ratios obtained for Al tolerance and sensitivity in the F 2 and backcross generations were 3 : 1 and 1 : 1, respectively. Test of allelism confirmed the same gene was conferring Al tolerance in both genotypes ('L-7903' and 'L-4602') as the F 1 was also tolerant and no segregation of tolerant : sensitive was recorded. These results indicated that Al tolerance is a monogenic dominant trait that can be easily transferred to agronomic bases through backcross breeding technique.

Molecular Scanning and Morpho-Physiological Dissection of Component Mechanism in Lens Species in Response to Aluminium Stress

“…Callose accumulation in roots has also been known as a sensitive indicator of A1 toxicity and can be readily detected by fluorescence microscopy [ 62 , 36 ]. In controls (absence of Al), resistant and sensitive genotypes showed a negligible fluorescence, whereas strong fluorescence signals (callose accumulation) were detected in roots after exposure to 74 and 148 μm Al for 48 h. Callose was observed slightly lower in resistant breeding lines (L-7903, L-4602 and ILWL-185) than in sensitive cultivars (BM-4, L-4147 and ILWL-436) ( Fig 6 ).…”

WITHDRAWN: Deep expression scrutiny provides genetic basis of aluminium tolerance in wild (Lens nigrican) and cultivated lentil (Lens culinaris Medik.)

“…These accessions were chosen based on previous studies, where L-4602 was found to be Al tolerant breeding line developed at Indian Agricultural Research Institute (IARI), India whereas BM-4 was found to be Al sensitive cultivar, which was developed from the cross between ILL-5888 (improved landrace) and ILL-5782 (breeding line) at International Center for Agricultural Research in the Dry Areas (ICARDA), Syria [7,90,91]. ILWL-15 is a mineral rich wild accession which was originated in France and was reported to be moderately tolerant to Al stress [7,92].…”

Mechanistic Association of Quantitative Trait Locus with Malate Secretion in Lentil (Lens culinaris Medikus) Seedlings under Aluminium Stress

“…Two common strategies to alleviate Al toxicity are: soil liming [ 4 , 5 ] and crop breeding to develop resistant genotypes [ 6 , 7 ]. Amongst these, development of Al-resistant genotypes is considered as the most effective, economical and environmentally acceptable strategy to maintain sustainable production under Al toxic soils.…”

“…Efficient screening technique plays an important role to get appropriate differential response in genotypes reflected via stress indicating parameters. In our previous Al stress studies, root re-growth, callose deposition, Al accumulation, antioxidant synthesis and organic acid exudation were efficiently used as identification parameters for Al toxicity resistance [ 7 , 15 , 16 , 17 ]. Yusuf et al [ 18 ] assessed Al-resistance of wheat cultivars based on photosynthetic (maximum quantum yield of PSII), osmotic (leaf water potential, electrolyte leakage) and physio-biochemical (lipid peroxidation, hydrogen peroxide production, antioxidant enzyme synthesis and proline content) traits.…”

“…Similarly, Carcamo et al [ 19 ] tested differential response of blueberry cultivars to Al stress using photosynthetic pigments, antioxidants, total phenols, starch and soluble sugars as marker traits. To date, limited studies have been taken up to decipher physiological and genetic bases of Al-resistance in lentil [ 7 ].…”