Variation in the Pathogenicity of Lily Isolates of Cucumber mosaic virus

Lee, Jin A; Choi, Seung‐Kook; Yoon, Ju Yeon; Hong, Jin Sung; Ryu, Ki Hyun; Lee, Sang Yong; Choi, Joongdae

doi:10.5423/ppj.2007.23.4.251

Cited by 17 publications

(15 citation statements)

References 25 publications

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“…The necrotic ringspot symptom on inoculated leaves has been reported in CMV lily isolates (CMV-Ly2 and-Ly8) on N. tabacum cv. 'Xanthi-nc', but necrotic ringspot symptom on upper non-inoculated leaves was only observed from CMVLy8 (Lee et al, 2007). CMV-Z, -RB, and CMV-ZM also differed significantly in their pathogenicity on P. angularis, Cu.…”

Section: Note Open Accessmentioning

confidence: 92%

“…CMV has the broadest host range of among known plant viruses, infecting more than 1,200 species of plants from monocotyledons to dicotyledons (Douine et al, 1979;Kaper and Waterworth, 1981). Various kinds of symptoms are produced by CMV on different hosts (Choi et al, 2004;Jeon et al, 2006;Kaper and Waterworth, 1981;Lee et al, 2007;Lee et al, 2008;Martelli and Russo, 1985;Oh et al, 2008). CMV is efficiently transmitted by more than 75 species of aphids including Myzus persicae and Aphis gossypii in a styletborne, nonpersistent manner (Palukaitis et al, 1992).…”

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confidence: 99%

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Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea

Kim¹,

Kwak²,

Lee³

et al. 2011

The Plant Pathology Journal

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A virus causing mottle and stunt symptom on Zea mays was observed around Ulleng-do, Korea and identified as Cucumber mosaic virus (CMV-ZM) based upon biological, serological, and molecular characteristics. In host range studies, the CMV-ZM isolate produced local lesions on Datura stramonium, Vigna unguiculata, Cucurbita moschata, Chenopodium amaranticolor, Ch. quinoa, whereas this isolate produced systemic mosaic on Nicotiana tabacum cv. 'Xanthi-nc', Capsicum annuum, Solanum lycopersicum, Solanum melongena, Cucurbita pepo, and Z. mays. In addition, chlorotic local rings on inoculated leaves along with severe mosaic, malformation, and fern leaf symptoms on upper systemic leaves were shown in N. glutinosa plants. Complete nucleotide sequences of each genomic RNA segment was determined and compared to those of the other CMV strains. Comparison of the nucleotide sequence of 1a open reading frame (ORF) revealed approximately 89.2-92.4% sequence identity with each CMV subgroup IA and IB strain, while showing only 78% sequence identity with CMV subgroup II. Nucleotide sequence analysis of RNA2 ORFs revealed 85.3-97.6% sequence identity with subgroup I. In ORFs of RNA3, levels of nucleotide sequence identities were higher than 92-99.2% with CMV subgroup I and lower than 82% with CMV isolates of subgroup II. These results suggest that CMV-ZM isolate is more closely related to subgroup I than subgroup II and therefore, CMV-ZM isolate might be classified into as CMV subgroup I based on biological and molecular analysis.

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Section: Note Open Accessmentioning

confidence: 92%

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confidence: 99%

Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea

Kim¹,

Kwak²,

Lee³

et al. 2011

The Plant Pathology Journal

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“…RNA 3 encodes the 3a protein (movement protein, MP) for viral movement and the capsid protein (CP) for encapsidation which is translated from a subgenomic RNA 4. CMV has an extremely broad host range of approximately 1,000 species including vegetables, cereals, flowering plants and trees (Gal-On et al, 1994;Lee et al, 2007;Palukaitis and Garcia-Arenal, 2003;Ryu et al, 1998).…”

Section: Abstract : African Impatiens Capsid Protein Cucumber Mosaimentioning

confidence: 99%

First report of Cucumber mosaic virus in African Impatiens (Impatiens walleriana) in Korea

Choi¹,

Choi²,

Kwon³

et al. 2015

Research in Plant Disease

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Virus-like symptoms including stunt, severe mosaic with malformation of leaves, fern-like leaves and abnormal petals were observed from an African impatiens (Impatiens walleriana) grown in a plant nursery in Icheon, Korea. Serological analysis using immuno-strip kits for viruses reported in African impatiens indicated that Cucumber mosaic virus (named CMV-Im) was a causal agent for the symptomatic African impatiens. Biological properties of CMV-Im were analyzed using responses of host plant species, suggesting that CMV-Im is a typical strain that belongs to CMV subgroup I. RT-PCR analysis verified CMV-Im infection from naturally infected African impatiens or mechanically inoculated some host species. Analysis of multiple alignments of CMV capsid protein (CP) sequences showed that CMV-Im shared high CP amino acids identities with other CMV strains. Phylogenetic tree analysis for the CP sequences of CMV-Im and representative CMV strains confirmed that CMV is a typical member of CMV subgroup I. To our knowledge, it is the first report of CMV in African impatiens in Korea. Keywords : African impatiens, Capsid protein, Cucumber mosaic virus, Immuno-strip, Sequence analysisImpatiens walleriana, commonly called 'African impatiens' or 'New Guinea impatiens', is a year-round outdoor blooming perennial, native to eastern Africa including Kenya, Tanzania and Mozambique. African impatiens can be bred easily by cuttings and the shoots also have high regenerating capacity after harvesting. African impatiens is frequently used for bedding and decoration of borders or used as pot plants in the world. Since 1990's, African impatiens plants have been grown in some gardens of Korea and have been distributed throughout the nation by commercial plant nurseries.Cucumber mosaic virus (CMV), a type species of the genus Cucumovirus in the family Bromoviridae, is a positive-sense single-stranded RNA virus with a tripartite genome (Palukaitis et al., 1992;Palukaitis and Garcia-Arenal, 2003). CMV strains can be divided into three subgroups, IA, IB and II (Roossink et al., 1999;Palukaitis and Garcia-Arenal, 2003). CMV is consisted of three genomic RNAs. RNA 1 encodes 1a protein that contains domains of methyltransferase and helicase for viral replication. RNA2 encodes 2a protein (the viral polymerase) and 2b protein that is essential for viral systemic movement and infectivity in some host plants (Ding et al., 1995;Hayes and Buck, 1990). RNA 3 encodes the 3a protein (movement protein, MP) for viral movement and the capsid protein (CP) for encapsidation which is translated from a subgenomic RNA 4. CMV has an extremely broad host range of approximately 1,000 species including vegetables, cereals, flowering plants and trees (Gal-On et al., 1994;Lee et al., 2007;Palukaitis and Garcia-Arenal, 2003;Ryu et al., 1998).In March 2014, virus-like symptoms including stunt, severe mosaic with malformation of leaves, fern-like leaves and abnormal petals were observed from an African impatiens plant grown in a plant nursery in Icheon, Korea ( 342(TBSV),...

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“…Most of them were not able to infect many species of tobacco and cucurbits (Jung et al 2000;Ryu et al 2002;Lee et al 2007) and they induced mild systemic symptoms on other herbaceous test plants (Kamińska 1996;Yamaguchi et al 2005;Lee et al 2007). It was reported that lily isolates Li (Ryu et al 2002) and Ly8 (Lee et al 2007) were able to infect Nicotiana tabaccum cv. Xanti-nc and/or N. glutinosa systemically, but they did not induce even local infection on Cucumis sativus plants which is considered to be a good propagation host for the virus.…”

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confidence: 95%

Cucumber mosaic virus groups IA and II are represented among isolates from naturally infected lilies

2010

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Four cucumber mosaic virus isolates (named Cas, CB, P26 and Simp2) found in naturally infected lily plants were characterized on the basis of their serological properties and the results of analysis of RNA3 sequence fragments containing coat protein and movement protein genes. The properties of lily isolates were compared with those of eight other virus isolates originating from dahlia, delphinium, impatiens, honeysuckle, cucumber and redcurrant plants. On the basis of the reaction with group-specific monoclonal antibodies and RNA3 sequence analysis, two lily isolates (Cas and CB) were classified to group I of CMV, similarly to all previously reported virus isolates found in lily plants. Surprisingly, sequences of coat protein and movement protein genes of two other lily isolates (P26 and Simp2) showed more than 98% similarity to CMV group II isolates, and only 77% similarity to group I isolates. Results of ELISA with CMV group II-specific monoclonal antibodies confirmed the classification of isolate Simp2 as a member of group II. Isolate P26 reacted neither with CMV group I nor with group IIspecific monoclonal antibodies. In order to explain the lack of reaction of P26 isolate with monoclonal antibodies, comparative analysis of predicted amino acid sequence of the coat protein was done. This revealed a mutation-change from alanine at position 138 to threonine, probably responsible for particular serological properties of isolate P26.Keywords CMV grouping . Lilium sp. . Sequence analysis . SerologyCucumber mosaic virus (CMV), the type member of genus Cucumovirus in the family Bromoviridae, is a positive-sense RNA plant virus with a tripartite genome. CMV has the widest host range among plant viruses encompassing more than 1,200 species in over 100 families of dicotyledonous and monocotyledonous plants (Palukaitis and García-Arenal 2003). Numerous CMV isolates described so far, can be divided into two major groups-I and II on the basis of serological data, nucleic acid hybridization, peptide mapping of the coat protein (CP) and/or sequence analysis of representative isolates (for review see: Palukaitis et al. 1992). More recently, further division of group I into groups IA and IB was proposed by Roossinck et al. (1999) based on phylogenetic analysis of the RNA3 5′ non-translated region (NTR) and CP gene.

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Variation in the Pathogenicity of Lily Isolates of Cucumber mosaic virus

Cited by 17 publications

References 25 publications

Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea

Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea

First report of Cucumber mosaic virus in African Impatiens (Impatiens walleriana) in Korea

Cucumber mosaic virus groups IA and II are represented among isolates from naturally infected lilies

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