Variation in Parasitoid Virulence of Tetrastichus brontispae during the Targeting of Two Host Beetles

Zhang, Hua-Jian; Lin, Ya‐Ping; Li, Hongyu; Rui, Wang; Fu, Lang; Jia, Qing-Chen; Hou, Youming

doi:10.3390/ijms22073581

Cited by 2 publications

(2 citation statements)

References 86 publications

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“…The equilibrium maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs) in modulating transcription and gene expression underscores the complexity of epigenetic regulation [ 20 , 21 , 22 , 23 , 24 , 25 ]. Our investigation focuses on the pupal endoparasitoid wasp Tetrastichus brontispae Ferrière (Hymenoptera: Eulophidae), which, in the absence of polydnavirus, relies on maternal venom as its primary virulence factor [ 26 , 27 ]. T. brontispae targets Chrysomelidae (Coleoptera) pests, including Octodonta nipae and Brontispa longissimi , in palm cultivation [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp

Jiang,

Zhou,

Cui

et al. 2024

Insects

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Endoparasitoids are insects that develop within other insects, employing unique strategies to enhance their offspring’s survival. They inject polydnavirus and/or venom into their hosts along with eggs, effectively suppressing the host’s immune system. Polydnavirus from Braconidae and Ichneumonidae wasps can integrate into the host’s genome to express viral genes using the host’s transcription systems. However, the ability of parasitoids without polydnavirus to manipulate host gene expression remains unclear. Lysine acetylation (LysAc), a post-translational modification critical for gene regulation, is hypothesized to be used by endoparasitoids lacking polydnavirus. We utilized the Chalcidoidea wasp Tetrastichus brontispae, which lacks polydnavirus, as an idiobiont endoparasitoid model to test this hypothesis, with pupae of the nipa palm hispid beetle Octodonta nipae as the host. Parasitism by T. brontispae resulted in the reduced expression of histone deacetylase Rpd3 and elevated levels of LysAc modification at histones H3.3K9 and H3.3K14 through proteomics and LysAc modification omics. The knockdown of Rpd3 increased the expression level of OnPPAF1 and OnPPO involved in the phenoloxidase cascade, leading to melanization in the host body whereby it resembled a mummified parasitized pupa and ultimately causing pupa death. This study enhances our understanding of how endoparasitoids employ histone acetylation to regulate immunity-related genes, offering valuable insights into their survival strategies.

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Section: Introductionmentioning

confidence: 99%

Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp

Jiang,

Zhou,

Cui

et al. 2024

Insects

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“…They were highly expressed in hemolymph except OnPPAF2, which shows low transcript abundance. However, knockdown of OnPPAF1 and OnPPAF3 showed a reduction in hemolymph phenoloxidase activity and inhibition of hemolymph melanization [ 51 , 52 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

The Entomopathogenic Nematodes H. bacteriophora and S. carpocapsae Inhibit the Activation of proPO System of the Nipa Palm Hispid Octodonta nipae (Coleoptera: Chrysomelidae)

Sanda

Hou

2022

Life

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Entomopathogenic nematodes are biocontrol agents of invasive insect pests in soil and cryptic habitats. Nipa palm hispid, Octodonta nipae, is a pest of palm trees in Sothern China. To address its increasing damage, environmentally friendly control methods are required. This study aimed to test efficacy of Heterorhabditis bacteriophora and Steinernema carpocapsae on O. nipae and investigated the influence of secondary metabolites, nematodes, and their isolated cuticles on the activation of O. nipae’s prophenoloxidase system using qPCR analysis. Our data revealed that O. nipae were less susceptible to H. bacteriophora than S. carpocapsae and penetrations of infective juveniles were higher with S. carpocapsae treatment than H. bacteriophora. Moreover, expression levels of the serine protease P56, prophenoloxidase activation factor 1, PPO and serine protease inhibitor 28 upon S. carpocapsae and H. bacteriophora infections were generally downregulated at all times. However, upon heating, the cuticles lost their inhibitory effects and resulted in upregulation of the PPO gene. Similarly, the addition of arachidonic acid reversed the process and resulted in the upregulation of the PPO gene compared to the control. Further work is needed to identify toxic substances secreted by these EPNs to evade O. nipae’s immune system.

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Variation in Parasitoid Virulence of Tetrastichus brontispae during the Targeting of Two Host Beetles

Cited by 2 publications

References 86 publications

Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp

Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp

The Entomopathogenic Nematodes H. bacteriophora and S. carpocapsae Inhibit the Activation of proPO System of the Nipa Palm Hispid Octodonta nipae (Coleoptera: Chrysomelidae)

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