Variation in Natural Abundance of 15N among Plant Parts and in 15N/14N Fractionation during N2 Fixation in the Legume-Rhizobia Symbiotic System

Yoneyama, T.; Fujita, Kazuo; Yoshida, Tomio; Matsumoto, Tetsuo; Kambayashi, Iwao; Yazaki, Jinya

doi:10.1093/oxfordjournals.pcp.a077165

Cited by 108 publications

(61 citation statements)

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“…1), indicating that Nj-fixed by the cyanobacteria with which they were co-cultivated has contributed to the nitrogen budget of the plants. In most cases, the 5^^N values were lower for shoots than for roots, which is in agreement with the findings of Yoneyama et al (1986) who reported more negative values of iJ^^N in shoots of some legumes than in roots and argued that this could be due to isotope discrimination during the transport of fixed N from nodules. We observed large differences in iJ'^N values within one wheat cultivar when co-cultured with different cyanobacteria ( Fig.…”

Section: Discussionsupporting

confidence: 91%

Colonization of wheat (Triticum vulgare L.) by N₂‐fixing cyanobacteria

Gantar

Kerby

Rowell³

et al. 1995

New Phytologist

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SUMMARYTwo cyanobacterial soil isolates, Nostoc 2S9B and Anabaena C5, that had previously been shown to form different types of association with the roots of wheat plants grown in liquid culture, were tested for heterotrophic nitrogenase aetivity and the ability to colonize the roots of plants grown in sand. Nostoc 2S9B showed substantial nitrogenase activity when associated with the roots of plants grown in liquid culture in medium free of combined N, even with the roots maintained and with assays performed in the dark (29 % of the rate shown by rootassociated Nostoc 2S9B grown and assayed in the light). When grown heterotrophically in the dark, at the expense of fructose, free-living Nostoc 2S9B showed a similar nitrogenase activity to root-associated Nostoc 2S9B in the dark. In contrast, Anabaena C5 showed no nitrogenase activity in the dark, under these conditions.When three different wheat cultivars were grown in sand that had previously been surface-inoculated with Nostoc 2S9B or with the cultured symbiotic cyanobactedum Nostoc LBGl, isolated from the bryophyte Anthoceros, there was colonization of the plant roots; there was no colonization of roots by Anabaena C5 under these conditions. Some increases in plant biomass and nitrogen content were observed, but these were dependent on the wheat cultivar and cyanobacterial inoculum used.Wheat plants grown in sand that had been pre-inoculated with Nostoc 2S9B, Nostoc LBGl or Anabaena CS in medium free of combined N had lower 5'^N values in both roots and shoots than plants grown under identical conditions without a cyanobaeterial inoculum. The observed '^N/^''N fractionation indicates that N., fixed by the cyanobacteria contributed to the nitrogen economy of the wheat plants, irrespective of whether they were closely associated with the plant roots.

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Section: Discussionsupporting

confidence: 91%

Colonization of wheat (Triticum vulgare L.) by N₂‐fixing cyanobacteria

Gantar

Kerby

Rowell³

et al. 1995

New Phytologist

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“…Using plants grown in N-free medium (Steele et al, 1983;Bergersen et al, 1986;Yoneyama et al, 1986) have reported that the 'B' value of a particular legume may vary with rhizobium strain. Doughton et al (1992) Okito et al (2004) found that there were large differences in 'B' value of either the shoot tissue or the whole soybean plants between the different strains.…”

Section: Introductionmentioning

confidence: 99%

Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean

Guimarães¹,

Morais

Urquiaga

et al. 2008

Sci. agric. (Piracicaba, Braz.)

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In commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15 N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15 N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of d 15 N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15 N-isotope dilution in soils enriched with 15 N. To evaluate N 2 fixation by soybean, three non-N 2 -fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N 2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 ‰ and -0.50 ‰, while those inoculated with B. elkanii were -3.67 ‰ and -1.0 ‰, for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N 2 fixation. 15 N, e estimativas da contribuição da FBN derivado do uso da técnica de diluição isotópica de 15 N em solo enriquecido com 15 N. Para avaliar a fixação de N 2 pela soja três plantas referenciam foram crescidas nas mesmas condições. Independente da estirpe de Bradyrhizobium, não foi observada diferença para matéria seca, massa de nódulos e N total entre solo marcado e não marcado. A fixação de N 2 em soja crescida nas duas condições de marcação do solo foi semelhante. Os valores médios de 'B' para plantas de soja inoculadas com estirpes de B. japonicum foram, em média, de -1,84 ‰ e -0,50 ‰ enquanto as inoculadas com B. elkanii apresentaram médias de -3,67 ‰ e -1,00 ‰, para parte aérea e planta inteira, respectivamente. Finalmente, o valor 'B' para a cultura da soja variou consideravelmente em função da estirpe de Bradyrhizobium inoculada, sendo mais importante quando se utiliza somente a parte aérea da planta para estimar a proporção do N da planta derivado da fixação de N 2 . Palavras-chave: Bradyrhizobium elkanii, Bradyrhizobium japonicum, Glycine max, valor de 'B' Biological N 2 fixation in soybean 517

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“…The other three legume species were intermediate between these two for both nodule mass ratio and whole-plant d 15 N. Thus, d 15 N patterns among legume species provided evidence for a positive relationship between N 2 fixation and nodule mass ratio. Taking the nonnodulating legume S. parahyba as a reference plant (Pons et al, 2007) and assuming a discrimination of 1‰ during N 2 fixation (Yoneyama et al, 1986), we estimated the proportion of plant N acquired by N 2 fixation to be 84% for O. macrocalyx, 73% for A. adinocephala, 52% for D. retusa, 46% for I. punctata, and 0% for S. parahyba. However, these values should be considered as indicative only, because the non-N 2 -fixing species included in the study showed a relatively large range of plant d 15 N, similar to previous observations for non-N 2 -fixing tropical tree species (Guehl et al, 1998;Cernusak et al, 2007aCernusak et al, , 2009cPons et al, 2007).…”

mentioning

confidence: 99%

Responses of Legume Versus Nonlegume Tropical Tree Seedlings to Elevated CO2 Concentration

et al. 2011

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We investigated responses of growth, leaf gas exchange, carbon-isotope discrimination, and whole-plant water-use efficiency (W P ) to elevated CO 2 concentration ([CO 2 ]) in seedlings of five leguminous and five nonleguminous tropical tree species. Plants were grown at CO 2 partial pressures of 40 and 70 Pa. As a group, legumes did not differ from nonlegumes in growth response to elevated [CO 2 ]. The mean ratio of final plant dry mass at elevated to ambient [CO 2 ] (M E /M A ) was 1.32 and 1.24 for legumes and nonlegumes, respectively. However, there was large variation in M E /M A among legume species (0.92-2.35), whereas nonlegumes varied much less (1.21-1.29). Variation among legume species in M E /M A was closely correlated with their capacity for nodule formation, as expressed by nodule mass ratio, the dry mass of nodules for a given plant dry mass. W P increased markedly in response to elevated [CO 2 ] in all species. The ratio of intercellular to ambient CO 2 partial pressures during photosynthesis remained approximately constant at ambient and elevated [CO 2 ], as did carbon isotope discrimination, suggesting that W P should increase proportionally for a given increase in atmospheric [CO 2 ]. These results suggest that tree legumes with a strong capacity for nodule formation could have a competitive advantage in tropical forests as atmospheric [CO 2 ] rises and that the water-use efficiency of tropical tree species will increase under elevated [CO 2 ].

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Variation in Natural Abundance of 15N among Plant Parts and in 15N/14N Fractionation during N2 Fixation in the Legume-Rhizobia Symbiotic System

Cited by 108 publications

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Colonization of wheat (Triticum vulgare L.) by N₂‐fixing cyanobacteria

Colonization of wheat (Triticum vulgare L.) by N₂‐fixing cyanobacteria

Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean

Responses of Legume Versus Nonlegume Tropical Tree Seedlings to Elevated CO2 Concentration

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Variation in Natural Abundance of 15N among Plant Parts and in 15N/14N Fractionation during N2 Fixation in the Legume-Rhizobia Symbiotic System

Cited by 108 publications

References 0 publications

Colonization of wheat (Triticum vulgare L.) by N2‐fixing cyanobacteria

Colonization of wheat (Triticum vulgare L.) by N2‐fixing cyanobacteria

Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean

Responses of Legume Versus Nonlegume Tropical Tree Seedlings to Elevated CO2 Concentration

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Colonization of wheat (Triticum vulgare L.) by N₂‐fixing cyanobacteria

Colonization of wheat (Triticum vulgare L.) by N₂‐fixing cyanobacteria