Variation in microbial community from predominantly mesophilic to thermotolerant and moderately thermophilic species in an industrial copper heap bioleaching operation

Acosta, Mauricio; Galleguillos, Pedro A.; Ghorbani, Yousef; Tapia, Pierina; Contador, Yasna; Velásquez, Antonio; Espoz, Cristian; Pinilla, Carlos; Demergasso, Cecilia

doi:10.1016/j.hydromet.2014.09.010

Cited by 41 publications

(29 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular analysis in the leachate of industrially or naturally leaching systems shows that microbial consortia of multiple species occur and the genus Sulfobacillus and Leptospirillum are detected frequently (Okibe et al, 2003;Johnson et al, 2008;Acosta et al, 2014). The genus Sulfobacillus was described by Golovacheva andKaravaiko in 1978 (Golovacheva andKaravaȋko, 1978).…”

Section: Introductionmentioning

confidence: 99%

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Zhu

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor from L. ferriphilum. Data show that the number of attached S. thermosulfidooxidans on pyrite increases, if the pyrite had been pre-colonized by living biofilms of L. ferriphilum, while it decreases if the pre-colonized biofilms had been inactivated. Coaggregation between S. thermosulfidooxidans and L. ferriphilum occurs during the dual-species biofilm formation, but different effects on bioleaching were noted, if the preculture of L. ferriphilum had been different. If L. ferriphilum had been pre-colonized on a pyrite, significantly negative effect was shown. However, if the two species were simultaneously inoculated into a sterile leaching system, the bioleaching efficiency was better than that of a pure culture of S. thermosulfidooxidans. The effect might be related to a metabolic preference of S. thermosulfidooxidans. If S. thermosulfidooxidans performed leaching in a filtered pyrite leachate from L. ferriphilum, the cells preferred to oxidize RISCs instead of ferrous ion and the number of attached cells decreased compared with the control. This study gives an indication that in a short-term multi-species leaching system the role of S. thermosufidooxidans may be related to the time of its introduction.

show abstract

Section: Introductionmentioning

confidence: 99%

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Zhu

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Instead of a sole species, microbial populations exhibited substantial diversity in stirred-tank, mineral-processing bioreactors and ore leaching heaps. Microbial community analysis of the leachate has shown that the microbial bioleaching communities varied in commercial mineral leaching operations, but the genus of Sulfobacillus can be detected frequently [6][7][8]. The genus Sulfobacillus was first reported by Golovacheva and Karavaiko in 1978 [9].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Biofilm Formation on Pyrite by Sulfobacillus thermosulfidooxidans

Sand

Zhang

2016

Minerals

View full text Add to dashboard Cite

Abstract:Bioleaching is the mobilization of metal cations from insoluble ores by microorganisms. Biofilms can enhance this process. Since Sulfobacillus often appears in leaching heaps or reactors, this genus has aroused attention. In this study, biofilm formation and subsequent pyrite dissolution by the Gram-positive, moderately thermophilic acidophile Sulfobacillus thermosulfidooxidans were investigated. Five strategies, including adjusting initial pH, supplementing an extra energy source or ferric ions, as well as exchanging exhausted medium with fresh medium, were tested for enhancement of its biofilm formation. The results show that regularly exchanging exhausted medium leads to a continuous biofilm development on pyrite. By this way, multiply layered biofilms were observed on pyrite slices, while only monolayer biofilms were visible on pyrite grains. In addition, biofilms were proven to be responsible for pyrite leaching in the early stages.

show abstract

“…Recently, Acosta et al ( 2014 ) extracted RNA from the pregnant liquor solution samples, also found that Leptospirillum and S. thermosulfidooxidans are the most abundant thermoacidophiles in the deeper areas of heaps (up to 54 m); their relative abundance increases with the age of the heap, and where there is pH lower than 2, high Eh, and up to 3 g/L of Cu (Demergasso et al 2005 ; Remonsellez et al 2009 ). Similar conditions were present in our experiments of As tolerance and biooxidation, while temperature was 48°C…”

Section: Discussionmentioning

confidence: 99%

Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps

2015

View full text Add to dashboard Cite

Soluble arsenic (As) in acidic feed solution may inhibit the copper (Cu) bioleaching process within mine heaps. To clarify the effect of soluble arsenic on the live biomass and bioxidative activity in heaps, toxicological assays were performed using a synthetic feed solution given by a mine company. The microorganisms had previously been isolated from two heap samples at up to 66 m depth, and cultured using specific media for chemolithotrophic acidophiles (pH 1–2) and moderate thermophiles (48°C), for arsenic tolerance assay. The four media with the highest biomass were selected to assay As-resistance; one culture (Q63h) was chosen to assay biooxidative activity, using a heap sample that contained chalcopyrite and covellite. We found that 0.5 g/L of As does not affect living biomass or biooxidative activity on Cu sulfides, but it dissolves Cu, while As precipitates as arsenic acid (H3AsO4·½H2O). The arsenic tolerant community, as identified by 16S rDNA gene sequence analysis, was composed of three main metabolic groups: chemolithotrophs (Leptospirillum, Sulfobacillus); chemolithoheterotrophs and organoheterotrophs as Acidovorax temperans, Pseudomonas alcaligenes, P. mendocina and Sphingomonas spp. Leptospirillum spp. and S.thermosulfidooxidans were the dominant taxa in the Q63–66 cultures from the deepest sample of the oldest, highest-temperature heap. The results indicated arsenic resistance in the microbial community, therefore specific primers were used to amplify ars (arsenic resistance system), aio (arsenite oxidase), or arr (arsenate respiratory reduction) genes from total sample DNA. Presence of arsB genes in S. thermosulfidooxidans in the Q63–66 cultures permits H3AsO4-As(V) detoxification and strengthens the community’s response to As.

show abstract

Variation in microbial community from predominantly mesophilic to thermotolerant and moderately thermophilic species in an industrial copper heap bioleaching operation

Cited by 41 publications

References 35 publications

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Enhancement of Biofilm Formation on Pyrite by Sulfobacillus thermosulfidooxidans

Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps

Contact Info

Product

Resources

About