Variation in larval gut bacteria between insecticide-resistant and -susceptible populations of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Gracy, R. Gandhi; Malathi, V. M.; Jalali, S. K.; Jose, V. Lyju; Thulasi, A.

doi:10.1007/s12600-016-0547-9

Cited by 8 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteobacteria accounted for about 68% of the reads. This result is not surprising and is in agreement with findings in other insects, including Lepidoptera [32,53,54]. Two families, Enterobacteriaceae and Enterococcaceae, were detected as the most sequence-rich taxa and are commonly and consistently present in insects' gut, especially in Lepidoptera.…”

Section: Discussionsupporting

confidence: 90%

“…However, similar results were found to ours in a previous study where resistant tobacco budworms had a higher bacterial density and diversity when fed on Bt cotton compared to non-Bt cotton [52]. Using culture and DGGE analyses, Gracy et al [53] also found that H. armigera larvae collected from Bt cotton had a higher bacterial diversity compared to larvae from other plants (Pigeonpea, Chickpea and Tomato) and were also the most resistant to various insecticides (spinosad, emamectin benzoate, cypermethrin, thiodicarb). It is well-known that diet is an important factor in shaping an insect's microbiome [20], and therefore, it is not surprising that insects feeding on non-Bt versus Bt cotton have differences in their microbiota.…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Bacterial Microbiota of Field-Collected Helicoverpa zea (Lepidoptera: Noctuidae) from Transgenic Bt and Non-Bt Cotton

et al. 2021

View full text Add to dashboard Cite

The bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), is an important agricultural pest in U.S. cotton and is managed using transgenic hybrids that produce insecticidal proteins from the bacterium, Bacillus thuringiensis (Bt). The reduced efficacy against H. zea caterpillars of Bt plants expressing Cry toxins is increasing in the field. In a first step towards understanding Bt cotton–bollworm–microbiota interactions, we investigated the internal bacterial microbiota of second–third stadium H. zea collected in the field from non-Bt versus Bt (WideStrike) cotton in close proximity (in North Carolina, USA). The bacterial populations were analyzed using culture-dependent and -independent molecular approaches. We found that WideStrike samples had a higher bacterial density and diversity per larva than insects collected from non-Bt cotton over two field seasons: 8.42 ± 0.23 and 5.36 ± 0.75 (log10 colony forming units per insect) for WideStrike compared to 6.82 ± 0.20 and 4.30 ± 0.56 for non-Bt cotton for seasons 1 and 2, respectively. Fifteen phyla, 103 families, and 229 genera were identified after performing Illumina sequencing of the 16S rRNA. At the family level, Enterobacteriaceae and Enterococcaceae were the most abundant taxa. The Enterococcaceae family was comprised mostly of Enterococcus species (E. casseliflavus and another Enterococcus sp.). Members of the Enterococcus genus can acidify their environment and can potentially reduce the alkaline activation of some Bt toxins. These findings argue for more research to better understand the role of cotton–bollworm–bacteria interactions and the impact on Bt toxin caterpillar susceptibility.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Bacterial Microbiota of Field-Collected Helicoverpa zea (Lepidoptera: Noctuidae) from Transgenic Bt and Non-Bt Cotton

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In the corn earworm Helicoverpa zea and the old-world bollworm Helicoverpa armigera, an increase in bacterial diversity was also observed in the presence of Bt-producing transgenic plants. More specifically, a proliferation of bacteria of the Enterococcaceae family and more particularly of species Enterococcus casseliflavus and Klebsiella oxytoca was reported (Gracy et al 2016;Deguenon et al 2021). Similarly, exposure to Bt increased the bacterial density within the gut and hemolymph of Spodoptera littoralis larvae and the cabbage moth Plutella xylostella (Caccia et al 2016;Li et al 2021).…”

Section: Insecticidesmentioning

confidence: 98%

Reciprocal interactions between anthropogenic stressors and insect microbiota

Antonelli

Duval

Luis

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

Insects play many important roles in nature due to their diversity, ecological role, and impact on agriculture or human health. They are directly influenced by environmental changes and in particular anthropic activities that constitute an important driver of change in the environmental characteristics. Insects face numerous anthropogenic stressors and have evolved various detoxication mechanisms to survive and/or resist to these compounds. Recent studies highligted the pressure exerted by xenobiotics on insect life-cycle and the important role of insect-associated bacterial microbiota in the insect responses to environmental changes. Stressor exposure can have various impacts on the composition and structure of insect microbiota that in turn may influence insect biology. Moreover, bacterial communities associated with insects can be directly or indirectly involved in detoxification processes with the selection of certain microorganisms capable of degrading xenobiotics. Further studies are needed to assess the role of insect-associated microbiota as key contributor to the xenobiotic metabolism and thus as a driver for insect adaptation to polluted habitats.

show abstract

“…Lactobacillus plantarum , 20,21 Pediococcus pentosaceus and Lactococcus lactis 27 also were proven to degrade organophosphate insecticides. According to several studies, alleles that confer insecticide resistance are positively correlated with the presence of insect symbionts, as was demonstrated in Culex pipiens , 30 Helicoverpa armigera 31 and Plutella xylostella 32 . Bacterial symbionts of the pest insects Bactrocera dorsalis 33 and Riptortus pedestris 34 have been shown to confer resistance to organophosphates through their fast degradation.…”

Section: Introductionmentioning

confidence: 95%

Does Leptinotarsa decemlineata larval survival after pesticide treatment depend on microbiome composition?

Stará

Hubert

2023

Pest Management Science

View full text Add to dashboard Cite

BackgroundThe microbiomes of some arthropods are believed to eliminate pesticides by chemical degradation or stimulation of the host immune system. The Colorado potato beetle (CPB; Leptinotarsa decemlineata) is an important agricultural pest with known resistance to used pesticides. We sought to analyze microbiome composition in CPB larvae from different sites and to identify the effect of pesticides on the microbiome of surviving and dead larvae after chlorpyrifos treatment in laboratory. Changes in Lactococcus lactis community in larvae treated with chlorpyrifos and fed by potato leaves with L. lactis cover were studied in manipulative experiment. The microbiome was characterized by sequencing the 16S RNA gene.ResultsThe microbiome of L. decemlineata larvae is composed of a few operational taxonomic units (OTUs) (Enterobacteriaceae, Pseudocitrobacter, Acinetobacter, Pseudomonas, L. lactis, Enterococcus, Burkholderia, and Spiroplasma leptinotarsae). The microbiome varied among the samples from eight sites and showed differences in profiles between surviving and dead larvae. The survival of larvae after chlorpyrifos treatment was correlated with a higher proportion of L. lactis sequences in the microbiome. The S. leptinotarsa profile also increased in the survived larvae, but this OTU was not present in all sampling sites. In manipulative experiments, larvae treated with L. lactis had 5‐fold lower mortality rates than untreated larvae.ConclusionThese results indicate that the microbiome of larvae is formed from a few bacterial taxa depending on the sampling site. A member of the gut microbiome, L. lactis, is believed to help overcome the toxic effects of chlorpyrifos in the larval gut.This article is protected by copyright. All rights reserved.

show abstract

Variation in larval gut bacteria between insecticide-resistant and -susceptible populations of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Cited by 8 publications

References 38 publications

Bacterial Microbiota of Field-Collected Helicoverpa zea (Lepidoptera: Noctuidae) from Transgenic Bt and Non-Bt Cotton

Bacterial Microbiota of Field-Collected Helicoverpa zea (Lepidoptera: Noctuidae) from Transgenic Bt and Non-Bt Cotton

Reciprocal interactions between anthropogenic stressors and insect microbiota

Does Leptinotarsa decemlineata larval survival after pesticide treatment depend on microbiome composition?

Contact Info

Product

Resources

About