Variation in Karyotype and 4C DNA Content in Six Species of Melocactus of the Family Cactaceae.

Das, Anath Bandhu; Mohanty, S.; Das, P.

doi:10.1508/cytologia.63.9

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…Melocactus curvispinus is a diploid cactus (2n=22; Das et al. , 1998), easy to distinguish morphologically by its depressed‐globose body, less than 30 cm tall at maturity.…”

Section: Methodsmentioning

confidence: 99%

Genetic variation and population structure of the mixed-mating cactus, Melocactus curvispinus (Cactaceae)

2001

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Genetic diversity was measured in the mixed-mating cactus, Melocactus curvispinus, in Venezuela. Allozyme diversity was surveyed in 19 putative loci over 18 populations. Compared to other plant taxa, this cactus is rich in polymorphic loci (Ps=89.5%), with high numbers of alleles per polymorphic locus (APs=3.82), but moderate levels of heterozygosity (Hes=0.145). Substantial levels of inbreeding were detected across loci and populations at macrogeographic (FIS=0.348) and regional levels (FIS=0.194-0.402). Moderate levels of genetic differentiation among populations were detected at macrogeographical (FST=0.193) and regional (FST=0.084-0.187) scales, suggesting that gene flow is relatively restricted, but increases within regions without topographic barriers. The population genetic structure observed for this cactus was attributed to, at least, three factors: short-distance pollination and seed dispersal, the mixed-mating condition of the species, and genetic drift. High genetic identities between populations (I=0.942) supported the conspecific nature of all populations surveyed. The levels and patterns of genetic structure observed for M. curvispinus were consistent with its mating system and gene dispersal mechanisms.

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“…Melocactus curvispinus is a diploid cactus (2n=22; Das et al. , 1998), easy to distinguish morphologically by its depressed‐globose body, less than 30 cm tall at maturity.…”

Section: Methodsmentioning

confidence: 99%

Genetic variation and population structure of the mixed-mating cactus, Melocactus curvispinus (Cactaceae)

2001

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“…As observed by Das et al (1998a , 1998b) and Das and Mohanty (2008) the Melocactus genus is characterized by significant differences in DNA content among species, which can be conveniently detected by flow cytometry to identify species in natural populations and germplasm banks. Variations in ploidy between species are related to loss or addition of repeats in the genome, which may reflect micro- and macroscopic alterations during genome evolution ( Das et al, 1998b ).…”

Section: Discussionmentioning

confidence: 99%

Anatomy, Flow Cytometry, and X-Ray Tomography Reveal Tissue Organization and Ploidy Distribution in Long-Term In Vitro Cultures of Melocactus Species

et al. 2020

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Cacti have a highly specialized stem that enables survival during extended dry periods. Despite the ornamental value of cacti and the fact that stems represent the main source of explants in tissue culture, there are no studies on their morpho-anatomical and cytological characteristics in Melocactus . The present study seeks to address the occurrence of cells with mixed ploidy level in cacti tissues. Specifically, we aim to understand how Melocactus stem tissue is organized, how mixoploidy is distributed when present, and whether detected patterns of ploidy change after long periods of in vitro culture. To analyze tissue organization, Melocactus glaucescens and Melocactus paucispinus plants that had been germinated and cultivated in vitro were analyzed for stem structure using toluidine blue, Xylidine Ponceau, Periodic Acid Schiff, ruthenium red, and acid floroglucin. To investigate patterns of ploidy, apical, medial, and basal zones of the stem, as well as, periphery, cortex, and stele (vascular tissue and pith) regions of the stem and root apexes from four- and ten-year old cultured in vitro were analyzed by flow cytometry. X-ray micro-computed tomography (XRµCT) was performed with fragments of stems from both species. The scarcity of support elements ( i.e. , sclereids and fibers) indicates that epidermis, hypodermis, and wide-band tracheids present in cortical vascular bundles and stele, as well as water stored in aquifer parenchyma cells along the cortex, provide mechanical support to the stem. Parenchyma cells increase in volume with a four-fold increase in ploidy. M. glaucescens and M. paucispinus exhibit the same pattern of cell ploidy irrespective of topophysical region or age, but there is a marked difference in ploidy between the stem periphery (epidermis and hypodermis), cortex, stele, and roots. Mixoploidy in Melocactus is not related to the age of the culture, but is a developmental trait, whereby endocycles promote cell differentiation to accumulate valuable water.

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“…a 26,46 pg em M. plumosa F.A.C.Weber), Das et al (2000), que estudaram quatro espécies de Astrophytum Lem. (que variaram de 9,23 pg a 10,12 pg), Das et al (1998aDas et al ( e 1998b …”

Section: Estudos Cariotípicosunclassified

Variação Cromossômica Em Cactáceas

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Variation in Karyotype and 4C DNA Content in Six Species of Melocactus of the Family Cactaceae.

Cited by 17 publications

References 20 publications

Genetic variation and population structure of the mixed-mating cactus, Melocactus curvispinus (Cactaceae)

Genetic variation and population structure of the mixed-mating cactus, Melocactus curvispinus (Cactaceae)

Anatomy, Flow Cytometry, and X-Ray Tomography Reveal Tissue Organization and Ploidy Distribution in Long-Term In Vitro Cultures of Melocactus Species

Variação Cromossômica Em Cactáceas

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