Abstract:Variation in growth, production and quality attributes of Physalis species under temperate ecosystem. Abstract-Introduction. Diverse fruit crops with a high value reduce the risk of crop failure and offer alternatives to farmers and the market. The scope of profitable production with such quality crops along with environmental concerns make the evaluation of new species desirable. The aim of our study was to determine the most appropriate species of Physalis for small-scale commercial production in the tempera… Show more
The objective of this study was to evaluate the influence of different types of sugar (white refined sugar, white crystal sugar, demerara sugar, brown sugar and coconut sugar) on the physicochemical characteristics, rheological properties and sensory acceptance of physalis (Physalis L) jelly. In addition, we evaluated the influence of the information on the acceptability of the product. It was found that the type of sugar greatly influences the physicochemical and rheological characteristics of physalis jelly which reflects differences in the product acceptability. Due to higher sensory acceptance, white refined, white crystal and demerara sugars are the most suitable for processing jellies. It was also found that the sugar type information and its benefits has no significant influence on sensory acceptance of physalis jelly and that consumers have a preference for a clearer, less sweet, more acidic and softer jelly.
The objective of this study was to evaluate the influence of different types of sugar (white refined sugar, white crystal sugar, demerara sugar, brown sugar and coconut sugar) on the physicochemical characteristics, rheological properties and sensory acceptance of physalis (Physalis L) jelly. In addition, we evaluated the influence of the information on the acceptability of the product. It was found that the type of sugar greatly influences the physicochemical and rheological characteristics of physalis jelly which reflects differences in the product acceptability. Due to higher sensory acceptance, white refined, white crystal and demerara sugars are the most suitable for processing jellies. It was also found that the sugar type information and its benefits has no significant influence on sensory acceptance of physalis jelly and that consumers have a preference for a clearer, less sweet, more acidic and softer jelly.
“…Physalis most recognizable as food crops are groundcherry (P. pruinosa), goldenberry (P. peruviana), and tomatillo (P. ixocarpa). P. pruinosa originated in Eastern North America, whereas P. peruviana and P. ixocarpa originated in Peru and Mexico, respectively (Singh et al 2014). All three of these Physalis species are primarily consumed as fresh fruit, but P. pruinosa and P. peruviana are also processed to make jams, juices, raisins, and snack products (Puente et al 2011).…”
Section: Introductionmentioning
confidence: 99%
“…Physalis pruinosa is of special interest for our work because of its potential as a specialty fruit crop that could provide farmers, namely in temperate regions based on its origins, with an additional source of income that would be further spurred by the growing consumer demand for new and unique fruits (Singh et al 2014). One advantage of P. pruinosa from an agricultural standpoint is it can be grown in a wide variety of soil types with successful crop production even in poor, sandy conditions (Wolff 1991).…”
Physalis pruinosa, also known as groundcherry, produces a small, yellow, highly nutritious edible fruit that is enveloped by a papery husk. In order for the potential of large-scale production of P. pruinosa fruit to be realized, undesirable characteristics, such as an unmanageable, sprawling growth habit and extensive fruit drop, need to be improved by exploiting approaches available through plant breeding, genetic engineering, and gene editing. In this study, we established plant regeneration and Agrobacterium tumefaciens-mediated methods to allow application of genetic engineering and gene editing of P. pruinosa. Cotyledon and hypocotyl explants from 7 to 8-day-old in vitro-grown seedlings were assessed for plant regeneration. Explants were cultured for 2 weeks on a Murashige and Skoog salts-based medium that contained 2 mg/L zeatin followed by transfer to medium containing 1 mg/L zeatin. Only hypocotyl explants regenerated shoots. Hypocotyl explants were infected with Agrobacterium tumefaciens strain AGL1 containing the pJL33 binary vector that has the green fluorescent protein reporter and neomycin phosphotransferase II (nptII) selectable marker genes. After cocultivation, explants were cultured on selective plant regeneration medium that contained 50, 100, 200, 250, and 300 mg/L kanamycin to determine the most effective level for efficient recovery of transgenic lines. Based on PCR analysis for the presence of the nptII gene, medium containing 200 mg/L kanamycin resulted in the highest transformation efficiency at 24%. This study sets the foundation for future genetic engineering and gene editing approaches for improvement of P. pruinosa. Key message Methodology for regeneration and transformation of Physalis pruinosa is a key component for the genetic improvement of this underutilized fruit crop for future agricultural production. Keywords AGL1 • Green fluorescent protein • Groundcherry • Kanamycin • Solanaceae Abbreviations GFP Green fluorescent protein GUS Beta-glucuronidase MS Murashige and Skoog nptII Neomycin phosphotranferase II
“…Physalis most recognizable as food crops are groundcherry (P. pruinosa), goldenberry (P. peruviana), and tomatillo (P. ixocarpa). P. pruinosa originated in Eastern North America, whereas P. peruviana and P. ixocarpa originated in Peru and Mexico, respectively (Singh et al 2014). All three of these Physalis species are primarily consumed as fresh fruit, but P. pruinosa and P.…”
Section: Introductionmentioning
confidence: 99%
“…peruviana are also processed to make jams, juices, raisins, and snack products (Puente et al 2011). P. pruinosa is of special interest for our work because of its potential as a specialty fruit crop that could provide farmers, namely in temperate regions based on its origins, with an additional source of income that would be further spurred by the growing consumer demand for new and unique fruits (Singh et al 2014). One advantage of P. pruinosa from an agricultural standpoint is it can be grown in a wide variety of soil types with successful crop production even in poor, sandy conditions (Wolff 1991).…”
Physalis pruinosa, also known as groundcherry, produces a small, yellow, highly nutritious edible fruit that is enveloped by a papery husk. In order for the potential of large-scale production of P. pruinosa fruit to be realized, undesirable characteristics, such as an unmanageable, sprawling growth habit and extensive fruit drop, need to be improved by exploiting approaches available through plant breeding, genetic engineering, and gene editing. In this study, we established plant regeneration and Agrobacterium tumefaciens-mediated methods to allow application of genetic engineering and gene editing of P. pruinosa. Cotyledon and hypocotyl explants from 7 -8-dayold in vitro-grown seedlings were assessed for plant regeneration. Explants were cultured for 2 weeks on a Murashige and Skoog salts-based medium that contained 2 mg/L zeatin followed by transfer to medium containing 1 mg/L zeatin. Only hypocotyl explants regenerated shoots.Hypocotyl explants were infected with Agrobacterium tumefaciens strain AGL1 containing the pJL33 binary vector that has the green fluorescent protein (GFP) reporter and neomycin phosphotransferase II (nptII) selectable marker genes. After cocultivation, explants were cultured on selective plant regeneration medium that contained 50, 100, 200, 250, and 300 mg/L kanamycin to determine the most effective level for efficient recovery of transgenic lines. Based on rooting of regenerated shoots on selective medium, GFP visualization, and PCR analysis for the presence of the nptII gene, medium containing 200 mg/L kanamycin resulted in the highest transformation efficiency at 24%. This study sets the foundation for future genetic engineering and gene editing approaches for improvement of P. pruinosa.All rights reserved. No reuse allowed without permission.was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
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