Variation in cell-specific rates of leucine and thymidine incorporation by marine bacteria with high and with low nucleic acid content off the Oregon coast

Longnecker, Krista; Sherr, Barry F.; Sherr, Evelyn B.

doi:10.3354/ame043113

Cited by 60 publications

(60 citation statements)

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“…This agrees with other studies showing that significant growth rates, sometimes equal to those of HNA cells, were also measured in LNA cells (Scharek and Latasa, 2007;Williams et al, 2008). LNA cells were found to be active members of the Hprok community, not only in terms of protein synthesis, but also in cell division, as shown in groups sorted following thymidine incorporation (Longnecker et al, 2006). LNA cells in surface layers may have advantages over other groups, assuming that, like in the open Atlantic, they are dominantly represented by Roseobacter and SAR11 clade (Mary et al, 2006).…”

Section: Importance Of Lna Cells In the Leucine Incorporation Ratessupporting

confidence: 80%

“…LNA cells incorporated significant amounts of leucine at all the stations and depths sampled (1.2-12.5 × 10 −21 mol leucine cell −1 h −1 ), and the rates were in the range of previously published values using saturating concentrations of leucine (Lebaron et al, 2002;Longnecker et al, 2006). The abundance of LNA cells reported for Pacific samples increased proportionally towards the basin station, with cell-specific rates decreasing vertically between 0 and 200 m (Longnecker et al, 2006). In the Mediterranean Sea, our data showed a greater vertical variability than longitudinal variability (Fig.…”

Section: Importance Of Lna Cells In the Leucine Incorporation Ratesmentioning

confidence: 61%

“…and slope areas (e.g. Lebaron et al, 2001;Longnecker et al, 2005Longnecker et al, , 2006. In such oligotrophic environments, LNA cells are therefore an important dynamic group, whose role in the microbial communities and in C cycling is far from negligible.…”

Section: Importance Of Lna Cells In the Leucine Incorporation Ratesmentioning

confidence: 99%

“…Longnecker et al, 2005Longnecker et al, , 2006Mary et al, 2006Mary et al, , 2008bHill et al, 2010). LNA cells also play a role in the microbial food web, as the growth and grazing of LNA cells are more or less balanced (Scharek and Latasa, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Changes in the nature and quantity of resources and grazers affect both cytometric groups (Servais et al, 1999;Nishimura et al, 2005;Longnecker et al, 2010). Temperature selectively affects some groups along the water column, longitudinal gradients, or seasons (Longnecker et al, 2006;Moran and Calvo-Díaz, 2009), but the identification of a single factor driving HNA and LNA cell distribution is difficult, due to the simultaneous variation of biogeochemical variables along trophic gradients (Moran et al, 2010).…”

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confidence: 99%

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Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

et al. 2011

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Abstract. The surface of the Mediterranean Sea is a lowphosphate-low-chlorophyll marine area where marine heterotrophic prokaryotes significantly contribute to the biogeochemical cycles of all biogenic elements such as carbon, notably through the mineralization of dissolved organic compounds. Cell-specific leucine incorporation rates were determined in early summer in the open stratified Mediterranean Sea. The bulk leucine incorporation rate was on average 5 ± 4 pmol leu l −1 h −1 (n = 30). Cell-specific 3 Hleucine incorporation rates were assayed using flow cytometry coupled to cell sorting. Heterotrophic prokaryotes (Hprok) were divided into cytometric groups according to their side scatter and green fluorescence properties: high nucleic acid containing cells (HNA) with high scatter (HNAhs) and low scatter (HNA-ls) and low nucleic acid containing cells (LNA). Cell-specific leucine incorporation rates of these cytometric groups ranged from 2 to 54, 0.9 to 11, and 1 to 12 × 10 −21 mol cell −1 h −1 , respectively. LNA cells represented 45 to 63% of the Hprok abundance, and significantly contributed to the bulk leucine incorporation rates, from 12 to 43%. HNA/LNA ratios of cell-specific leucine incorporation were on average 2.0 ± 0.7 (n = 30). In surface layers (from 0 m down to the deep chlorophyll depth, DCM), cell-specific rates of HNA-hs were elevated (7 and 13 times greater than LNA and HNA-ls, respectively). Nevertheless, on average HNA-hs (26%) and LNA (27%) equally contributed to the bulk leucine incorporation in these layers. Prochlorococcus cells were easily sorted near the DCM and displayed cell-specific leucine incorporation rates ranging from 3 to 55 × 10 −21 mol leu cell −1 h −1 , i.e. as high asCorrespondence to: A. Talarmin (agathe.talarmin@univmed.fr) HNA-hs'. These sorted groups could therefore be defined as key-players in the process of leucine incorporation into proteins. The mixotrophic features of certain photosynthetic prokaryotes and the high contribution of LNA cells to leucine incorporation within the microbial communities of the Mediterranean could be reinforced.

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Section: Importance Of Lna Cells In the Leucine Incorporation Ratessupporting

confidence: 80%

Section: Importance Of Lna Cells In the Leucine Incorporation Ratesmentioning

confidence: 61%

Section: Importance Of Lna Cells In the Leucine Incorporation Ratesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

et al. 2011

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Limiting resources for soil microbial growth in climate change simulation treatments in the subarctic

Yuan,

Na,

Hicks

et al. 2023

Ecology

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The microbial use of resources to sustain life and reproduce influences for example, decomposition and plant nutrient provisioning. The study of “limiting factors” has shed light on the interaction between plants and their environment. Here, we investigated whether carbon (C), nitrogen (N), or phosphorus (P) was limiting for soil microorganisms in a subarctic tundra heath, and how changes in resource availability associated with climate change affected this. We studied samples in which changes in resource availability due to climate warming were simulated by the addition of birch litter and/or inorganic N. To these soils, we supplied factorial C (as glucose), N (as NH4NO3), and P (as KH2PO4/K2HPO4) additions (“limiting factor assays,” LFA), to determine the limiting factors. The combination of C and P induced large growth responses in all soils and, combined with a systematic tendency for growth increases by C, this suggested that total microbial growth was primarily limited by C and secondarily by P. The C limitation was alleviated by the field litter treatment and strengthened by N fertilization. The microbial growth response to the LFA‐C and LFA‐P addition was strongest in the field‐treatment that combined litter and N addition. We also found that bacteria were closer to P limitation than fungi. Our results suggest that, under a climate change scenario, increased C availability resulting from Arctic greening, treeline advance, and shrubification will reduce the microbial C limitation, while increased N availability resulting from warming will intensify the microbial C limitation. Our results also suggest that the synchronous increase of both C and N availability might lead to a progressive P limitation of microbial growth, primarily driven by bacteria being closer to P limitation. These shifts in microbial resource limitation might lead to a microbial targeting of the limiting element from organic matter, and also trigger competition for nutrients between plants and microorganisms, thus modulating the productivity of the ecosystem.

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Processing of humic-rich riverine dissolved organic matter by estuarine bacteria: effects of predegradation and inorganic nutrients

et al. 2014

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Variation in cell-specific rates of leucine and thymidine incorporation by marine bacteria with high and with low nucleic acid content off the Oregon coast

Cited by 60 publications

References 58 publications

Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

Limiting resources for soil microbial growth in climate change simulation treatments in the subarctic

Processing of humic-rich riverine dissolved organic matter by estuarine bacteria: effects of predegradation and inorganic nutrients

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