Variation in biofilm formation among blood and oral isolates of Candida albicans and Candida dubliniensis

Villar-Vidal, María; Marcos‐Arias, Cristina; Eraso, Elena; Quindós, Guillermo

doi:10.1016/j.eimc.2011.06.009

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…It also agreed with [37] found all tested isolates of C. albicans produced biofilm on polystyrene. [38] Found that total of 198 of 327 (60.6%) Candida species isolates were biofilm-positive.…”

Section: Biofilm Formation Of C Albicanssupporting

confidence: 75%

Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

2017

ijs

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Biofilm formation (BF) is one of the most important virulence factors of Candida spp. The aim of this study was to detect the prevalence of genes responsible in biofilm formation of C. albicans by conventional PCR technique. Among 49 vaginal specimens (VC), C. albicans was the most predominant species in percentage 22/49 (45%) and 27(55%) were non albicans. Out of 47 oral specimens (OS), 22/47(47%) were C. albicans, whereas 25(53%) were non albicans. At the present study; all C. albicans were biofilm producers with variable strength, out of 44 BF producers, 18 (40.9%) were low biofilm (LBF) with significant differences (P<0.05) between HVS and OS, 25 (56.8%) moderate or high biofilm (HBF) and just one isolate from oral was strong (very high) biofilm in percentage about 2.3% with no significant differences between HVS and OS.Molecular study of virulence genes correlated with biofilm C. albicans show that HWP1, ALS1, ALS3, SAP5, PLB1 and LIP8 genes were detected in 100% of C. albicans from vaginal and oral infections. Keywords ISSN: 0067-2904Iraqi Journal of Science, 2017, Vol. 58, No.2C, pp: 988-

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Section: Biofilm Formation Of C Albicanssupporting

confidence: 75%

Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

2017

ijs

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“…A produção de enzimas extracelulares é, particularmente, considerada como o maior fator de virulência para o desenvolvimento das infecções oportunistas, em especial na candidíase oral 5,7,9 . Entre as espécies fúngicas oportunísticas também consideradas como produtoras de enzimas hidrolíticas, estão Cryptococcus neoformans, Saccharomyces cerevisiae, Malassezia furfur e Rhodotorula rubra [15][16][17][18] .…”

Section: Discussionunclassified

“…Dentre os principais fatores de virulência encontrados na Candida spp., estão: a termotolerância; a presença de adesinas, as quais se destacam pela sua importância na adesão ao hospedeiro; a expressão de genes de resistência aos antifúngicos, bem como a produção de enzimas extracelulares, tais como fosfolipases, proteinases e hemolisinas 3,[5][6][7] .…”

Section: Introductionunclassified

Estudo comparativo entre a produção de fosfolipases extracelulares e proteinases do gênero Candida isoladas a partir de infecções de cavidade oral

Andreola

Demathé

Galafassi

et al. 2016

Rev. odontol. UNESP

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Resumo Introdução A habilidade da Candida spp. em produzir enzimas proteolíticas, tais como fosfolipase e proteinases, tem um papel importante na patogenicidade destas leveduras. Objetivo Determinar as espécies causadoras das infecções orais por Candida spp., além de investigar a atividade in vitro das fosfolipases e proteinases em isolados clínicos do gênero Candida, provenientes de pacientes com candidíase oral. Material e método Isolados de Candida spp., pertencentes à Coleção de Cultivos Fúngicos do Laboratório de Microbiologia e Patologia Oral do Departamento de Odontologia da Faculdade da Serra Gaúcha, foram analisados. Produção de fosfolipases foi analisada utilizando-se Ágar gema de ovo. Liberação de proteinases foi medida utilizando-se extrato de levedura adicionado à albumina bovina. Resultado Um total de 35 isolados clínicos do gênero Candida foi testado. C. albicans foi a espécie predominante (77%). Os demais isolados identificados foram: C. parapsilosis (20%) e C. tropicalis (2%). Ao comparar a atividade de fosfolipase do grupo C. albicans com o grupo Candida não-albicans, foi encontrada diferença significativa (P=0,04). Não foi encontrada diferença significativa entre a C. albicans e a C. não-albicans, para a produção de proteinase. A liberação de proteinase foi significativamente maior quando comparada à produção de fosfolipase para o gênero Candida (P=0,04). Diferença estatisticamente significativa foi encontrada quando a atividade de fosfolipase e proteinase da C. albicans foi comparada à atividade das espécies de C. não-albicans (P=0,02). Conclusão Diferentes quantificações de fosfolipase extracelular e atividade de proteinase têm sido atribuídas aos isolados clínicos de C. albicans quando comparados a outras espécies de Candida.

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“…Several factors, including host and surface properties, artificial saliva and different environmental conditions affect biofilm formation in C. albicans (25-27). In addition, biofilm formation also differed among different species of Candida (28). In our study, similar to other studies, all isolates of C. albicans were able to produce biofilms in vitro (28-30).…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole inCandida albicans

Mahmoudabadi

Zarrin

Kiasat

2014

Jundishapur J Microbiol

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Background:The ability of Candida albicans to form biofilms and adhere to host tissues and biomaterial surfaces is an important factor in its pathogenesis. One of the main characteristics of biofilms is their resistance to broad-spectrum anti-microbial drugs.Objectives:In the present study the formation of biofilm by C. albicans from different sources was evaluated. In addition, the minimum biofilm inhibitory concentration (MBIC) for two antifungals was evaluated.Materials and Methods:In total, 120 isolates of C. albicans from different sources (patients with vaginitis, patients with candiduria, bucal cavity and environmental surfaces) were collected. Biofilm formation was determined by the 96-well micro-titeration plate method. MBIC testing was also performed, using the calorimetric indicator resazurin for amphotericin B and fluconazole.Results:The results indicated that 100% of C. albicans isolates from different sources had the ability to form biofilms in vitro. Amongst these isolates, 83.3% of isolates had the maximum potential (4+) to form biofilms, while only one (0.9%) of isolates had the minimum ability (1+) to form biofilms. Our results showed that 65.0% of the tested isolates are sensitive to amphotericin B at amounts lower than 10 µg/mL, while only 26.7% are sensitive to fluconazole (had MBIC < 10 µg/mL).Conclusions:Although biofilm formation was detected in all tested isolates, there were differences in the ability to form biofilms between isolates from different sources. In addition, there were differences in the MBIC against the two examined antifungals, amphotericin B and fluconazole.

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Variation in biofilm formation among blood and oral isolates of Candida albicans and Candida dubliniensis

Cited by 20 publications

References 39 publications

Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

Estudo comparativo entre a produção de fosfolipases extracelulares e proteinases do gênero Candida isoladas a partir de infecções de cavidade oral

Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole inCandida albicans

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