Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

doi:10.24996/ijs.2017.58.2c.3

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The current investigation concurred with Mohammed et al (2017) who discovered that all C. albicans isolates tested developed biofilm on polystyrene with varying strength.…”

Section: Molecular Identification Of C Albicanssupporting

confidence: 88%

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Mahmoud¹,

Yassein²

2023

AAVS

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The aims of this study are to identify mycotic agents, assess the prevalence of mycotic mastitis, and evaluate the virulence of C. albicans that was isolated from Mastitic goats. To investigate the prevalence of mycotic mastitis, related to goats in Baghdad, 166 lactating goats were examined and 332 milk samples were collected from ( January -July) 2022. 10 ml of milk were collected in sterile test tubes, an equal volume of milk and CMT reagent were gently shaken in a paddle and the reaction was noticed within (10-15) seconds. The clinical form of mastitis accounted 9.35%, while the subclinical form accounted 52.4%, with positive (+ve) California mastitis test (CMT) results which are high significant. All milk samples were cultured on Sabouraud dextrose agar (SDA) for (3-5) days at 37 ºC. According to the result, 46.34% of the isolates had mycotic mastitis, with 33 isolates (34.74%) belonging to mold and 62 isolates (65.26%) returning to yeasts. Candida albicans had the highest percentage (high significant) of yeast isolation of (67.74%) with 42 isolates which were detected by using conventional methods such as the germ tube test, urease production, Chlamydospore formation, and chromogenic medium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 100% of C. albicans isolates. The diagnosis was confirmed using 25 of the most virulent C. albicans isolates, by polymers chains reaction (PCR) to detect the KER1 gene revealed that all 25 samples were positive with the product size (658bp). Phospholipases B1 (PLB1) and the secretion of aspartyl proteinase 1 (SAP1) gene were used to detect C. albicans virulence with 100% and 80% accuracy, respectively, while Hyphal Wall Protein 1 (HWP1) gene was not detected. It is expected that these C. albicans isolates contained a significant proportion of virulence factors that were linked to pathogenicity and the severity of infection. There findings suggest that poor sanitation and a reduction in goat health and nutrition were to blame for the high morbidity incidence of mycotic mastitis in Iraqi goats.

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“…The current investigation concurred with Mohammed et al (2017) who discovered that all C. albicans isolates tested developed biofilm on polystyrene with varying strength.…”

Section: Molecular Identification Of C Albicanssupporting

confidence: 88%

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Mahmoud¹,

Yassein²

2023

AAVS

View full text Add to dashboard Cite

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“…Unfortunately, techniques like single and direct PCR or multiplex PCR have not been widely adopted despite being more sensitive and specific and having a quicker turnaround time [30]. Earlier studies showed that the expression of HWP1 and genes from the ALS, SAP, LIP, and PLB gene families is connected to the development of biofilm on mucosal surfaces [24]. Fungal species that belong to Cryptococcus, Candida, Aspergillus, Histoplasma, and Pneumocystisare are responsible for more than 90% of all reported fungal-related deaths [25].…”

Section: Conventionalmentioning

confidence: 99%

Molecular Detection of Candida tropicalis Isolated from Buffalo's Genitalia in Wasit Province

Al-abedi,

Hussien,

Ali

2023

Egyptian Journal of Veterinary Sciences

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C ANDIDA tropicalis produces biofilms and other virulence factors like attachment to endothelial and buccal epithelial cell and release of lytic enzymes such as hemolysins, phospholipases, proteinases, and transformation from buds to hyphae (also known as morphogenesis). The aim of this study was to identify Candida tropicalis using API 20 Candida as well as using conventional polymers chain reaction technique for the detection of the genotype and virulence in isolates causing mycotic vaginitis in buffaloes in Wasit Province during the period (May 2021 to October 2021). In this study, one hundred vaginal swab samples were collected from buffalo. The results showed that Candida species were found in 40 out of 100 (40%) of the mycotic vaginal swabs and 30/40 (75%) of the isolated Candida species showed positive results using API 20 Candida test for Candida tropicalis. PCR amplification of the 18S rRNA gene for identification of Candida tropicalis revealed that this gene was detected in 16/30 samples (53.3%). Phospholipase (PLB) gene and hyphal formation (CPH1) gene were both found in 10/16 (62.5%) and 8/16 (50%) of samples respectively. In conclusion, the PCR was a useful tool and excellent alternative method for the early detection and diagnosis of the most frequent Candida species causing infection of genital tract in buffalo where culture was not required.

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Molecular Detection of Candida albicans Virulence Genes That Isolated from Periodontitis Patients in Al-Hillah City

Obais,

Al-shukri,

Al-Rubaye

2023

Medical Journal of Babylon

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Background: An inflammatory condition known as periodontitis causes the tooth’s supporting tissues to be destroyed over time. One of the current dental problems is its high prevalence and detrimental consequences on quality of life. An oral candidiasis lesion often develops as a result of the development of a complex biofilm containing Candida albicans and other bacteria. Objectives: The objective of this study was to detect C. albicans virulence gene agglutinin-like sequence 1 and hyphal wall protein 1 (ALS1 and HWP1) by molecular method from clinical oral infection sample. Materials and Methods: One hundred fifty oral swabs were obtained from periodontitis patients who attended to a specific dental health center and outpatient clinics of dentistry in Al-Hillah city, Iraq during the period from April 2022 to September 2022. Patient age ranges from 5 to 72 years. The sample was taken by disposable cotton swabs. This swab was cultured on various culture media including CHROM agar and Sabouraud’s dextrose agar for isolation of C. albicans. After that genomic DNA was extracted from confirmed colony. Virulence genes (ALS1 and HWP1) were detected by polymerase chain reaction technique. Results: The result showed that out of 150 samples 25 (16.66%) belong to C. albicans isolated; also the result showed that ALS1 gene detected in a rate 15 (22.05%) while HWP1 detected in a rate 19 (27.94%). Conclusion: The Als1 and HWP1 virulence genes were expressed in most isolates of C. albicans which suggests that the ALS1 and HWP1 protein play an important role in the pathogenesis of infection.

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Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

Cited by 4 publications

References 39 publications

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Molecular Detection of Candida tropicalis Isolated from Buffalo's Genitalia in Wasit Province

Molecular Detection of Candida albicans Virulence Genes That Isolated from Periodontitis Patients in Al-Hillah City

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