Variation in activity and heterogeneity of bovine milk proteases with stage of lactation and somatic cell count

Larsen, Lotte Bach; McSweeney, P.L.H.; Hayes, Michael; Andersen, Jannike Mørch; Ingvartsen, Klaus Lønne; Kelly, Alan L.

doi:10.1016/j.idairyj.2005.01.009

Cited by 38 publications

(33 citation statements)

References 24 publications

(51 reference statements)

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“…During mastitis, the endogenous proteolytic activity in milk increases substantially [15,27,28] and it is known that plasmin can hydrolyse β-casein, α S2 -casein and α S1 -casein [29] which explains the presence of peptides from α S1 -casein and β-casein in milk from the infected group. The results presented here, from investigation of naturally occurring mastitis, are in agreement with investigations of experimental induction of mastitis with LTA [15] or lipopolysaccharide [28] in which plasmin activity in milk increased and casein derived peptides were identified by reverse phase HPLC [15] or 2 dimension electrophoresis and mass spectroscopy [28] during mastitis.…”

Section: Discussionmentioning

confidence: 99%

A peptidomic approach to biomarker discovery for bovine mastitis

Mansor

Mullen

Barrett

et al. 2012

Farm Animal Proteomics

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Bovine mastitis is usually caused by either Gram positive or Gram negative bacteria, reducing the quantity and quality of milk produced. This investigation using capillary electrophoresis and mass spectroscopy, studied peptides in milk from cows with clinical mastitis in comparison to milk from healthy cows to identify biomarkers for mastitis. In addition, the milk peptidome from udders infected with Gram positive Staphylococcus aureus (S. aureus) or with Gram negative Escherichia coli (E. coli), was examined to assess differential diagnosis between the causative agent. Comparison of the peptidome between healthy (n = 10) and mastitic milk (n = 27) identified 154 peptides for a biomarker panel which in a model for diagnosis of mastitis showed 100% sensitivity and specificity. β-casein and α s1 casein provided the majority of peptides identified in this model. The peptidome comparison of milk from mastitis cases caused by S. aureus (n = 8) or E. coli (n = 11) revealed a biomarker panel of 47 peptides which discriminated between cause of infection with a sensitivity of 75% and a specificity of 100%. β-casein fragments were the most common of the peptides in this model.

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Section: Discussionmentioning

confidence: 99%

A peptidomic approach to biomarker discovery for bovine mastitis

Mansor

Mullen

Barrett

et al. 2012

Farm Animal Proteomics

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“…It hydrolyses the four types of caseins and is capable of catalysing milk coagulation (Kaminogawa et al 1980;Larsen et al 1996;McSweeney et al 1995), which may explain the presence of clots in mastitic milk . Its level has been positively correlated with SCC (Larsen et al 2006;O'Driscoll et al 1999;Somers et al 2003). Procathepsin D, the proenzyme of cathepsin D, and cathepsin appear to have remained partially active after commercial pasteurization processes .…”

Section: Elastasementioning

confidence: 97%

Mastitis impact on technological properties of milk and quality of milk products—a review

Maréchal

Thiéry²,

Vautor

et al. 2011

Dairy Science & Technol.

148

139

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The consequences of mastitis on the technological properties of milk and on the quality of milk products are widely reported in the literature. Besides, recent advances have shed light on the mechanisms involved in the udder response and subsequent milk changes in mastitis cases. This review gives an update on the literature regarding the impact of mastitis on milk composition and processing properties and collates recent data regarding the mechanisms involved in mastitis effects. It is an attempt to link field observations and experimental studies in order to better understand how mastites affect so dramatically the technological properties of milk. Both bovine and small ruminant milks are considered and a special emphasis is given on the role of staphylococci, streptococci, and Escherichia coli, the most common causative agents of mastitis.

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“…Pyorala and Kaartinen (1988) found a decrease in plasmin activity during the transition from colostrum to milk. Larsen et al (2006) reported that the activity of the acid milk protease, cathepsin D, is significantly lower in colostrum than in milk.…”

Section: Proteinasesmentioning

confidence: 99%

Composition and properties of bovine colostrum: a review

McGrath

Fox

McSweeney

et al. 2015

Dairy Sci. & Technol.

299

263

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Colostrum is the initial milk secreted by mammals following parturition, the composition and physicochemical properties of which are highly dynamic and variable. The composition and physicochemical properties of colostrum during the initial postpartum period has not been systematically reviewed for many years, although the topic remains of interest both to milk producers and processors. In this article, the current understanding of the composition of colostrum, i.e. carbohydrates, proteins, growth factors, enzymes, enzyme inhibitors, nucleotides and nucleosides, cytokines, fats, vitamins and minerals, is reviewed. In addition, the physicochemical properties, i.e. pH and buffering capacity, colour, density and specific gravity, osmotic pressure, somatic cell count, properties of casein micelles, ethanol stability and rennet coagulation properties are discussed, as well as the effects of heat-treating colostrum.

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Variation in activity and heterogeneity of bovine milk proteases with stage of lactation and somatic cell count

Cited by 38 publications

References 24 publications

A peptidomic approach to biomarker discovery for bovine mastitis

A peptidomic approach to biomarker discovery for bovine mastitis

Mastitis impact on technological properties of milk and quality of milk products—a review

Composition and properties of bovine colostrum: a review

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