In situ nuclear DNA content was estimated in a number of taxa at intergeneric, interspecific and intervarietal levels (Nagato et al. 1981, Dhillon and Miksche 1982, Sharma and Mukhopadhyay 1984, Mukherjee and Sharma 1986, Watson 1987, Das and Das 1994. Interspecific nuclear DNA content depends largely on repetitive and nonrepetitive sequences of the genome (Bennet et al. 1977, Ress andNarayan 1977). The genus Mammillaria of the family Cactaceae is widely distributed in Mexico, South of USA, Greater and Lesser Antilles and Coast of Venezuela (Cullmann et al. 1986). It has 62 species in India with varieties, cultivars and forms of horticultural importance . The Mammillarias are fairly small cacti, globular or elongated with small flowers arranged usually in a ring around the crown. Fruits are smooth, juicy, club shaped, with berries mostly of brilliant red colour (Heywood 1985). The chromosome analysis reported earlier deals with mainly of mitotic studies in different species of Mammillaria that showed 2n=22 in M. bravoae and M. elongata (Remski 1954). Various facets of researches on chromosome and DNA is gaining importance. To ascertain the diversity in DNA and meiotic chromosomes among the species of Mammillaria, understanding of the interspecific variations, if any, is necessary. The meiotic behaviour and chiasma frequency are the major basic information in the conventional breeding programme for creation of new hybrids of horticultural interest. Interspecific chromosome pairing behaviour, genomic compatibility and nuclear DNA content have not been studied in the genus Mammillaria earlier. The present study principally deals with the 4C DNA estimation in relation to genomic behaviour in 8 species of Mammillaria.
Materials and methodsFresh and young flower buds of 8 species of Mammillaria namely M. asteriflora Cels, M. bella Backbg., M bravoae Craig, M brevispina Boed., M. collinsii (B. & R.) Orcutt, M. elongata DC., M klissingiana Boed. and M matudae H. Bravo were collected from the experimental cactus garden of Regional Plant Resource Centre, Bhubaneswar having more than 550 species of cacti.For Feulgen cytophotometric estimation of 4C DNA, ten fixed root-tips of each species were hydrolysed in 1N HC1 for 12 min at 60°C, washed in distilled water and stained in Schiff's reagent for 2 hr at 14°C; each root-tip squash was prepared in 45% acetic acid. Ten scorings were made from each slide and 4C DNA content was estimated in metaphase chromosomes using Nikon Optiphot microscope with microspectrophotometer following the method of Sharma and Sharma (1980) and applying monochromatic light at 550 nm. In situ DNA values obtained on the basis of optical density were converted to picograms (pg) using Van't Hof's (1965) 4C nuclear DNA values 67.1 pg for Allium cepa as standard. To find out the significant differences of 4C DNA content among 8 species of Mammillaria, if any, analysis of variance (ANOVA) test was performed (Sokal and Rohlf 1973).Flower buds were fixed overnight in 1 : 3 propionic acid : ethanol at room tem...