Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves

Wani, S.A.; Hussain, I.; Nabi, Asha; Fayaz, I.; Nishikawa, Yoshikazu

doi:10.1111/j.1472-765x.2007.02235.x

Cited by 21 publications

(15 citation statements)

References 29 publications

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“…Several studies reported identifi cation of EHEC O157:H7 through various approaches to detect the toxin genes like, stx1, stx2, eaeA, rfbE, Z3276, hlyA and serogroupspecifi c genes wzx and wzy [Sharma & Dean-Nystrom, 2003;Wani et al, 2007]. Most of these genes are not very unique for the pathogenic serotypes like EHEC O157:H7.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

Qin¹,

Puthiyakunnon²,

Zhang³

et al. 2018

Pol. J. Food Nutr. Sci.

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Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (IMS) with loop-mediated isothermal amplifi cation (LAMP). The EHEC O157:H7 cells were separated with Dynabeads coated with anti-EHEC O157:H7 after a short enrichment for 4 h. Then, EHEC O157:H7 was identifi ed by LAMP assay for amplifying and detecting the rfbE gene, which is highly conserved in all EHEC O157:H7 strains and exhibits strain-specifi c gene expression. The LAMP method results analyzed with real time turbidity measurements showed a high specifi city and sensitivity, with a positive detection rate of amplifi cation of EHEC O157:H7 DNA diluted to a minimum equivalent concentration of 1.8 × 10 1 CFU/mL, which was 10 times more sensitive than the conventional PCR assay. The IMS followed with LAMP could capture and detect a bacterial concentration as low as 3×10 1 CFU/mL from the meat samples, which was close to the sensitivity of LAMP assay with pure culture. IMS combined with realistic LAMP method is a simple, rapid, highly specifi c gene amplifi cation technology that is suitable for implementing as a screening assay in basic laboratory and fi eld test for detecting food contamination. Unauthenticated Download Date | 5/11/18 5:54 AM

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Section: Discussionmentioning

confidence: 99%

Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

Qin¹,

Puthiyakunnon²,

Zhang³

et al. 2018

Pol. J. Food Nutr. Sci.

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“…A large number of reports on the isolation of aEPEC from a wide range of diarrheic and healthy animal species have been published. In most of the cases, these aEPEC strains have been identified in the course of characterization of A/E E. coli isolates among animals used for food production, such as cattle, sheep, goat, pig and poultry (Aidar et al, 2000;Aktan et al, 2004;Stephan et al, 2004;Blanco et al, 2005;Krause et al, 2005;Leomil et al, 2005;Yuste et al, 2006;Ishii et al, 2007;Wani et al, 2007). Moreover, the detection of aEPEC in domestic animals (dog and cat), horse, deer and marmoset has also been described (Beaudry et al, 1996;Goffaux et al, 2000;Carvalho et al, 2003;Nakazato et al, 2004;Krause et al, 2005;Ishii et al, 2007;A.…”

Section: Transmission and Reservoirmentioning

confidence: 99%

An overview of atypical enteropathogenicEscherichia coli

Hernandes

Elias

Vieira

et al. 2009

FEMS Microbiology Letters

263

216

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The enteropathogenic Escherichia coli (EPEC) pathotype is currently divided into two groups, typical EPEC (tEPEC) and atypical EPEC (aEPEC). The property that distinguishes these two groups is the presence of the EPEC adherence factor plasmid, which is only found in tEPEC. aEPEC strains are emerging enteropathogens that have been detected worldwide. Herein, we review the serotypes, virulence properties, genetic relationships, epidemiology, reservoir and diagnosis of aEPEC, including those strains not belonging to the classical EPEC serogroups (nonclassical EPEC serogroups). The large variety of serotypes and genetic virulence properties of aEPEC strains from nonclassical EPEC serogroups makes it difficult to determine which strains are truly pathogenic.

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“…As such, it is important to know, which virulence genes, phylogroups, serogroups, and antibiotic resistance patterns are present in commensal E. coli strains in yaks with diarrheic infections. Ruminants act as one of the most important reservoirs for pathogenic E. coli and lead to asymptomatic infections that can cause life-threatening complications in humans [5, 6]. Therefore, this bacterium in yaks with diarrhea may be a potential health risk if it is transmitted to humans via cross-contamination of water, food, carcasses, or feces [7].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance, serogroups, virulence genes, and phylogenetic groups of Escherichia coli isolated from yaks with diarrhea in Qinghai Plateau, China

Rehman¹,

Hui²,

Iqbal³

et al. 2017

Gut Pathog

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BackgroundRuminants serve as one of the most important reservoirs for pathogenic Escherichia coli. Infection with E. coli, a foodborne enteropathogen, can lead to asymptomatic infections that can cause life-threatening complications in humans. Therefore, from a clinical and human health perspective, it is important to know which virulence genes, phylogenetic groups, serogroups, and antibiotic resistance patterns are present in E. coli strains in yaks with diarrheic infections.MethodsTwo-hundred and ninety-two rectal swabs were collected from diarrheic yaks in Qinghai Plateau, China. The antimicrobial sensitivity of each resulting isolate was evaluated according to the disk diffusion method, and different PCR assays were performed for the detection of virulence genes and different phylogroups. Additionally, strains were allocated to different serogroups based on the presence of O antigen via the slide agglutination method.ResultsAmong the E. coli isolates tested, most of the isolates were multidrug resistant (97%) and harbored at least one virulence gene (100%). We observed ten virulence genes (sfa, eaeA, cnf1, etrA, papC, hlyA, aer, faeG, rfc, and sepA), of which sfa was the most commonly found (96.9%). Significant positive associations between some resistance phenotypes and virulence genes were observed (P < 0.05, OR > 1). The majority of the E. coli isolates belonged to phylogroup A (79.5%), and the others belonged to phylogroups B1 (7.5%), D (4.1%), B2 (5.8%), and F (0.7%). Among all the E. coli strains tested, serogroups O91 and O145 were the most prevalent, accounting for 15.4 and 14.4%, respectively.ConclusionsOur results suggest that yaks with diarrhea serve as a reservoir of pathogenic E. coli carrying various virulence genes and resistance phenotypes. Therefore, clinicians and relevant authorities must ensure the regulatory use of antimicrobial agents and prevent the spread of these organisms through manure to farm workers and food-processing plants.

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Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves

Cited by 21 publications

References 29 publications

Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

An overview of atypical enteropathogenicEscherichia coli

Antibiotic resistance, serogroups, virulence genes, and phylogenetic groups of Escherichia coli isolated from yaks with diarrhea in Qinghai Plateau, China

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