Variación genética y flujo de genes entre poblaciones de Crocodylus acutus (Crocodylia: Crocodylidae) en tres ríos del Pacífico Central, Costa Rica

Abstract

suggests that the population of the Tarcoles river is the source and those from Jesus Maria and Tusubres are the drains. There was a direct relationship between the genetic distance and the geographical distance (z =1.1449, r =0.9731, p< 0.0010). A conservation strategy for these crocodiles must consider the existence of the metapopulation between the three rivers and the importance of studying the genetics of the American Crocodile in the rest of the Pacific coast of Costa Rica, as well as over the entire distribution range of this species. Rev.

Genetic Diversity and Structure From Antillean Manatee (Trichechus manatus manatus) in the Southern Gulf of México: Comparison Between Connected and Isolated Populations

“…Amplifications were performed in a total volume of 22 µL, with 20 ng template DNA, 15 µL Platinum® PCR SuperMix (22 U/mL recombinant Taq DNA polymerase with Platinum® Taq Antibody, 22 mM Tris-HCl [pH 8.4], 55 mM KCl, 1.65 mM MgCl2, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, and stabilizers; Invitrogen), and 200 nM per primer. To visualize the PCR products, 2% agarose gel electrophoresis, stained with ethidium bromide, was used (Barril & Nates, 2012; Porras Murillo, Bolaños Montero, & Barr, 2008). Gels were photo-documented using Molecular Imager® Gel Doc™ (Bio-Rad).…”