Variable neuroprotective role of Filipendula ulmaria extract in rat hippocampus

Abstract

We evaluated the influence of an antioxidant-rich extract of Filipendula ulmaria L. on anxiety levels induced by nano-sized particles of different calcium phosphates. Rats in experimental groups were administered with either nano-sized hydroxyapatite, tricalcium phosphate, or amorphous calcium phosphate in the presence of Filipendula ulmaria extract. Appropriate behavioral tests were performed to assess anxiety levels, while oxidative status and apoptosis parameters were determined in the hippocampus samples. The applied calcium phosphates increased oxidative stress markers in hippocampal tissue, accompanied by an enhanced pro-apoptotic mechanism. Moreover, the hippocampal immunoreactivity for brainderived neurotrophic factor and GABAergic-A receptors was significantly lower following calcium phosphate nanoparticles intake. The observed functional and morphological alterations in the rat hippocampus occurred simultaneously with the anxiogenic response estimated in behavioral testing. The neuroprotective effect of Filipendulaulmaria was markedly manifested by the attenuation of oxidative damage induced by amorphous calcium phosphate and enhanced anti-apoptotic action in the rat hippocampus. The increased hippocampal immunoreactivity for brain-derived neurotrophic factor, GABAergic-A receptors and significant anxiolytic-like effects of Filipendula ulmaria may suggest a beneficial role of antioxidant supplementation in preventing anxiogenic response to nano-sized calcium phosphates.

Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and Filipendula ulmaria Extract in Rats

“…However, the benefits of therapeutic protocols with antioxidant supplementation and HBO were also confirmed by the increase in hippocampal BDNF relative expression (Figure 7). This is in accordance with the beneficial effects of antioxidant supplementation with matrine reported for neurobehavioral alterations in a mouse model of burn injury [38], as well as with our previous reports that showed the increase in BDNF content following prolonged administration of FU extract in rat hippocampus and prefrontal cortex [39,40]. On the other hand, the neuroprotective role of HBO therapy was previously reported in in vitro on spiral ganglion neurons [41] and in a clinical trial with delayed encephalopathy [42], but with the opposite effect on BDNF in dorsal horns of the spinal cord [17].…”

“…Antioxidant supplementation was performed, as previously described in detail [ 39 ], with Filipendula ulmaria extract [ 22 ], for seven consecutive days (also starting 24 h following the induction of burns), to achieve the final daily dose of 100 mg/kg b.w., in all groups that included this therapeutic approach. The final concentration of FU extract was achieved by free access to drinking water based on the total water intake on the previous day.…”

Simultaneous Administration of Hyperbaric Oxygen Therapy and Antioxidant Supplementation with Filipendula ulmaria Extract in the Treatment of Thermal Skin Injuries Alters Nociceptive Signalling and Wound Healing

“…Starting the day after the induction of thermal injury, antioxidant supplementation was performed, as previously described [ 28 ], with Filipendula ulmaria extract [ 24 ] dissolved in tap water to reach a final concentration of 100 mg/kg b.w. for seven consecutive days in the groups: BURNS + FU, BURNS + FU + HBO, and FU.…”