Variable Expression of GLIPR1 Correlates with Invasive Potential in Melanoma Cells

Abstract

GLI pathogenesis-related 1 (GLIPR1) was previously identified as an epigenetically regulated tumor suppressor in prostate cancer and, conversely, an oncoprotein in glioma. More recently, GLIPR1 was shown to be differentially expressed in other cancers including ovarian, acute myeloid leukemia, and Wilms’ tumor. Here we investigated GLIPR1 expression in metastatic melanoma cell lines and tissue. GLIPR1 was variably expressed in metastatic melanoma cells, and transcript levels correlated with degree of GLIPR1 promoter methylation in vitro. Elevated GLIPR1 levels were correlated with increased invasive potential, and siRNA-mediated knockdown of GLIPR1 expression resulted in reduced cell migration and proliferation in vitro. Immunohistochemical studies of melanoma tissue microarrays showed moderate to high staining for GLIPR1 in 50% of specimens analyzed. GLIPR1 staining was observed in normal skin in merocrine sweat glands, sebaceous glands, and hair follicles within the dermis.

Genomic profiling of invasive melanoma cell lines by array comparative genomic hybridization

“…According to previous studies, after the translocation of the GLIPR1 (GLI pathogenesis-related 1) protein to the cell surface, the soluble N-terminal domain of the molecule is exposed to the extracellular space, which can lead to invasion [48,49]. In the present study, gain of the GLIPR1 gene was observed specifically in invasive cell lines.…”

“…In the present study, gain of the GLIPR1 gene was observed specifically in invasive cell lines. The mRNA expression level of the GLIPR1 gene was notably higher in invasive cell lines than in noninvasive lines, confirming that upregulation of the gene is associated with increased invasive potential in melanoma [48].…”

Melanoma Genetics/Genomics

“…Expression of one factor called GLIPR1 was found to correlate with the invasive potential in melanoma cells, as demonstrated in an Original Research Article by Awasthi et al (12). …”

Related to testes-specific, vespid and pathogenesis protein-1 is regulated by methylation in glioblastoma

“…Previous studies have reported that RTVP-1 can be epigenetically regulated (10–13), but this has not been demonstrated in central nervous system tissue or tumors.…”