Related to testes-specific, vespid and pathogenesis protein-1 is regulated by methylation in glioblastoma

Jacoby, Elad; Yalon, Michal; Leitner, Moshe; Cohen, Yinon; Fisher, Tamar; Eder, Sarit; Amariglio, Ninette; Rechavi, Gideon; Cazacu, Simona; Xiang, Cunli; Mikkelsen, Tom; Brodie, Chaya; Toren, Amos

doi:10.3892/ol.2014.1829

Cited by 9 publications

(15 citation statements)

References 20 publications

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“…To examine whether the epigenetic status of GLIPR1 in DDP-sensitive and -resistant human lung adenocarcinoma A549/DDP cells was responsible for the high expression of GLIPR1 in A549/DDP cells [ 9 , 15 , 20 ], bisulfite sequencing primers were designed to amplify a 264 bp region 5′ of the transcription start site containing five CpG sites as previously reported. [ 15 ] GLIPR1 bisulfite sequencing data were obtained on a minimum of 5 clones prepared from each of both A549 and A549/DDP cells ( Fig 8A ). The GLIPR1 promoter sequences amplified from A549/DDP cells showed no differences in methylation as compared to A549 cells ( Fig 8B ).…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, the mRNA level of GLIPR1 in DDP-resistant H460/DDP cells was also significantly higher than that in DDP-sensitive H460 cells. GLIPR1 has been found to be expressed at high levels in astrocytic [ 15 – 19 , 23 ], wilms [ 20 ], acute myeloid leukemia [ 21 ], and melanoma [ 22 ] cancers and at low levels in prostate [ 8 – 10 ], and lung cancer [ 11 ]. The differential expression pattern is possibly due to the epigenetic status of GLIPR1 in different cancers.…”

Section: Discussionmentioning

confidence: 99%

“…The differential expression pattern is possibly due to the epigenetic status of GLIPR1 in different cancers. It has been found that DNA hypomethylation of the GLIPR1 gene promoter led to its overexpression in glioma [ 15 ] and wilms [ 20 ] tumors, whereas hypermethylation of the GLIPR1 gene promoter led to the downregulation of its mRNA expression in prostate cancer [ 9 ]. Thus, the epigenetic status of GLIPR1 in DDP-sensitive and -resistant human lung adenocarcinoma A549 cells was examined; however, there were no differences in the levels of methylation between A549 and A549/DDP cells.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, overexpression of GLIPR1 induced apoptosis of lung cancer cells [ 11 ] and prostate cancer cells by activating reactive oxygen species/the JNK pathway [ 12 ], downregulating c-Myc [ 13 ], or suppressing AURKA and TPX2 [ 14 ]. In contrast, GLIPR1 is overexpressed in astrocytic [ 15 – 19 ], wilms [ 20 ], acute myeloid leukemia [ 21 ], and melanoma [ 22 ] cancers. The overexpression of GLIPR1 increases glioma cell proliferation [ 18 – 19 , 23 ], whereas the downregulation of GLIPR1 decreases the proliferation of glioma [ 18 , 23 ] and melanoma [ 22 ] cells.…”

Section: Introductionmentioning

confidence: 99%

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GLIPR1 modulates the response of cisplatin-resistant human lung cancer cells to cisplatin

et al. 2017

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Background and objectiveChemotherapy drugs, such as cisplatin (DDP), improve the survival of patients with lung cancer by inducing apoptosis in cancer cells, which quickly develop resistance to DDP through uncharacterized mechanisms. Glioma Pathogenesis-Related Protein 1 (GLIPR1) plays an important role in cell proliferation, migration and apoptosis. However, the expression and function of GLIPR1 in mediating DDP resistance in human lung adenocarcinoma A549/DDP and human large cell lung cancer H460/DDP cells has not yet been reported.MethodsIn this study, real-time PCR (RT-PCR) and western blot were used to examine the mRNA and protein expression of GLIPR1, respectively. Bright-field microscopy, the cell counting kit-8 (CCK-8) assay, flow cytometry analysis and JC-1 dye were used to measure the cellular morphology, proliferation, apoptosis and mitochondrial membrane potential, respectively.ResultsCompared to human lung adenocarcinoma A549 cells, the mRNA and protein expression of GLIPR1 were significantly increased in DDP-resistant A549/DDP cells (p < 0.05). Similarly, the mRNA level of GLIPR1 in DDP-resistant H460/DDP cells was also significantly higher than that in DDP-sensitive H460 cells (p < 0.05). Silencing of GLIPR1 in A549/DDP and H460/DDP cells led to increased apoptosis via a mitochondrial signaling pathway following incubation with various concentrations of DDP. Furthermore, GLIPR1 downregulation markedly reduced the protein expression of Bcl-2, and increased the cleaved Poly (ADP-Ribose) Polymerase (PARP) and cleaved caspase-3 in DDP-resistant A549/DDP cells.ConclusionIn this study, we demonstrated for the first time that GLIPR1 could modulate the response of DDP-resistant A549/DDP and H460/DDP cells to cisplatin. Therefore, GLIPR1 deserves further investigation in the context of none-small lung cancer (NSCLC).

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GLIPR1 modulates the response of cisplatin-resistant human lung cancer cells to cisplatin

et al. 2017

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“…Glioma pathogenesis-related protein 1 (RTVP-1) has oncogenic features in glioblastoma (GBM; World Health Organization class IV) and highly expressed in GBMs [50]. The RTVP-1 promoter was hypomethylated in GBM.…”

Section: Epigenetic Alterations and Glioblastoma Progressionmentioning

confidence: 99%

Epigenetics of Glioblastoma Multiforme

2015

J Clinic Res Bioeth

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Aberrations in the epigenetic machinery of the genome may results the inactivation of critical genes and the epigenetic changes are important mechanisms in the evolution of malignancies that not only contributes to tumorigenesis but may also precede genetic changes. Several epigenetic mechanisms have been observed in glioblastomas including DNA hyper-methylation of genes, histone modifications including methylation and acetylation, nucleosomal rearrangement and dysregulation of noncoding RNA expression have been shown to play a critical role in the biology of glioblastomas and to contribute to the clinical outcome. This review examines the general role of epigenetic changes in the malignant process and focuses on the known epigenetic changes and development of new therapeutic strategies against these malignancies.

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A novel methylation signature predicts radiotherapy sensitivity in glioma

Feng

Shi

et al. 2020

Sci Rep

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Glioblastoma (GBM) is the most common and malignant cancer of the central nervous system, and radiotherapy is widely applied in GBM treatment; however, the sensitivity to radiotherapy varies in different patients. To solve this clinical dilemma, a radiosensitivity prediction signature was constructed in the present study based on genomic methylation. In total, 1044 primary GBM samples with clinical and methylation microarray data were involved in this study. LASSO-COX, GSVA, Kaplan–Meier survival curve analysis, and COX regression were performed for the construction and verification of predictive models. The R programming language was used as the main tool for statistical analysis and graphical work. Via the integration analysis of methylation and the survival data of primary GBM, a novel prognostic and radiosensitivity prediction signature was constructed. This signature was found to be stable in prognosis prediction in the TCGA and CGGA databases. The possible mechanism was also explored, and it was found that this signature is closely related to DNA repair functions. Most importantly, this signature could predict whether GBM patients could benefit from radiotherapy. In summary, a radiosensitivity prediction signature for GBM patients based on five methylated probes was constructed, and presents great potential for clinical application.

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Related to testes-specific, vespid and pathogenesis protein-1 is regulated by methylation in glioblastoma

Cited by 9 publications

References 20 publications

GLIPR1 modulates the response of cisplatin-resistant human lung cancer cells to cisplatin

GLIPR1 modulates the response of cisplatin-resistant human lung cancer cells to cisplatin

Epigenetics of Glioblastoma Multiforme

A novel methylation signature predicts radiotherapy sensitivity in glioma

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