Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Fernand, Francois; Rubinsky, Liel; Golberg, Alexander; Rubinsky, Boris

doi:10.1002/bit.24479

Cited by 14 publications

(14 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Defining the optimum conditions for specific-cell-line electroporation can be a labor-and cost-intensive procedure. To avoid this problem, a high-throughput method has been introduced to identify electroporation parameters for cell electroporation in a single step using a concentric electrode configuration (139).…”

Section: Wwwannualreviewsorg • Electroporation In Medicinementioning

confidence: 99%

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Yarmush

Golberg

Serša³

et al. 2014

Annu. Rev. Biomed. Eng.

711

443

View full text Add to dashboard Cite

When high-amplitude, short-duration pulsed electric fields are applied to cells and tissues, the permeability of the cell membranes and tissue is increased. This increase in permeability is currently explained by the temporary appearance of aqueous pores within the cell membrane, a phenomenon termed electroporation. During the past four decades, advances in fundamental and experimental electroporation research have allowed for the translation of electroporation-based technologies to the clinic. In this review, we describe the theory and current applications of electroporation in medicine and then discuss current challenges in electroporation research and barriers to a more extensive spread of these clinical applications.

show abstract

Section: Wwwannualreviewsorg • Electroporation In Medicinementioning

confidence: 99%

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Yarmush

Golberg

Serša³

et al. 2014

Annu. Rev. Biomed. Eng.

711

443

View full text Add to dashboard Cite

show abstract

“…Applications of electroporation were used first for water sterilization for industrial use to eliminate microorganisms. It is now being used as IRE for tumor therapy (25,26). The advantages of IRE for tumor ablation comprises the following facts: non-thermal, short, pulsed electric fields; nanometer-sized pores in phospholipid layer; disturbance of homoeostasis; induction of apoptosis; tissue selectivity, no thermal damage (which means extracellular matrix/fibrous structures remain intact as bile ducts, vascular structures, renal pelvis); sharp ablation margins; no heat sink effects; "short ablation times" (minutes); and peri-interventional delineation [CT, ultrasound, magnetic resonance imaging (MRI)].…”

Section: Irementioning

confidence: 99%

From diagnosis to therapy in lung cancer: management of CT detected pulmonary nodules, a summary of the 2015 Chinese-German Lung Cancer Expert Panel

Meyer

Pirker

et al. 2016

Transl. Lung Cancer Res.

View full text Add to dashboard Cite

Improved risk classification criteria and novel imaging approaches for screening populations are highly required as more than half of lung cancer cases are false positive during the initial screening round if the National Lung Screening Trial (NLST) demographic criteria [≥30 pack years (PY) of cigarettes, age ≥55 years] are applied. Moreover, if the NLST criteria are applied to the Chinese population a high number of lung cancer patients are not diagnosed due to non-smoking related risk factors in China. The primary goal in the evaluation of pulmonary nodules (PN) is to determine whether they are malignant or benign. Volumetric based screening concepts such as investigated in the Dutch-Belgian randomized lung cancer screening trial (NELSON) seem to achieve higher specificity. Chest CT is the best imaging technique to identify the origin and location of the nodule since 20% of suspected PN found on chest X-ray turn out to be non-pulmonary lesions. Moreover, novel state-of-the-art CT systems can reduce the radiation dose for lung cancer screening acquisitions down to a level of 0.1 mSv with improved image quality to novel reconstruction techniques and thus reduce concerns related to chest CT as the primary screening technology. The aim of the first part of

show abstract

“…It is, moreover, cumbersome to estimate the local electric field distribution for different systems because of changes in impedance/conductance during electroporation protocols due to membrane resealing. To facilitate the optimization processes, a concentric electrodes electroporation system that allows for determining IRE/RE electric field threshold for bacteria inactivation in a single experiment has been previously introduced [18]. There is still a need for an experimental system that can offer a determination of the RE/no-EP and electroporation mediated molecular transport parameters in a single experiment.…”

Section: Introductionmentioning

confidence: 99%

Single-step electrical field strength screening to determine electroporation induced transmembrane transport parameters

Blumrosen

Abazari

Golberg

et al. 2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

The design of effective electroporation protocols for molecular delivery applications requires the determination of transport parameters including diffusion coefficient, membrane resealing, and critical electric field strength for electroporation. The use of existing technologies to determine these parameters is time-consuming and labor-intensive, and often results in large inconsistencies in parameter estimation due to variations in the protocols and setups. In this work, we suggest using a set of concentric electrodes to screen a full range of electric field strengths in a single test to determine the electroporation-induced transmembrane transport parameters. Using Calcein as a fluorescent probe, we developed analytical methodology to determine the transport parameters based on the electroporation-induced pattern of fluorescence loss from cells. A monolayer of normal human dermal fibroblast (NHDF) cells were pre-loaded with Calcein and electroporated with an applied voltage of 750 V with 10 and 50 square pulses with 50 μs duration. Using our analytical model, the critical electric field strength for electroporation was found for the 10 and 50 pulses experiments. An inverse correlation between the field strength and the molecular transport time decay constant, and a direct correlation between field strength and the membrane permeability were observed. The results of this work can simplify the development of electroporation-assisted technologies for research and therapies.

show abstract

Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Cited by 14 publications

References 12 publications

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

From diagnosis to therapy in lung cancer: management of CT detected pulmonary nodules, a summary of the 2015 Chinese-German Lung Cancer Expert Panel

Single-step electrical field strength screening to determine electroporation induced transmembrane transport parameters

Contact Info

Product

Resources

About