Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Yarmush, Martin L.; Golberg, Alexander; Serša, Gregor; Kotnik, Tadej; Miklavčič, Damijan

doi:10.1146/annurev-bioeng-071813-104622

Cited by 686 publications

(454 citation statements)

References 176 publications

(180 reference statements)

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“…However, intentional transient permeabilization of the membrane is also exploited as a means to introduce genes or drugs into an organism, and targeted permanent disruption of plasma membranes is an effective means to eliminate specific cells (1)(2)(3).…”

mentioning

confidence: 99%

Imaging the dynamics of individual electropores

Sengel

Wallace

2016

Proc. Natl. Acad. Sci. U.S.A.

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Electroporation is a widely used technique to permeabilize cell membranes. Despite its prevalence, our understanding of the mechanism of voltage-mediated pore formation is incomplete; methods capable of visualizing the time-dependent behavior of individual electropores would help improve our understanding of this process. Here, using optical single-channel recording, we track multiple isolated electropores in real time in planar droplet interface bilayers. We observe individual, mobile defects that fluctuate in size, exhibiting a range of dynamic behaviors. We observe fast (25 s −1 ) and slow (2 s −1 ) components in the gating of small electropores, with no apparent dependence on the applied potential. Furthermore, we find that electropores form preferentially in the liquid disordered phase. Our observations are in general supportive of the hydrophilic toroidal pore model of electroporation, but also reveal additional complexity in the interactions, dynamics, and energetics of electropores.droplet interface bilayer | electroporation | toroidal pores | optical single-channel recording | lipid bilayers

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mentioning

confidence: 99%

Imaging the dynamics of individual electropores

Sengel

Wallace

2016

Proc. Natl. Acad. Sci. U.S.A.

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“…7,8 Additionally, continuous-type RF irradiation generates thermal ablative effects in target tissues by continuously delivering HF energy over set conduction times, whereas pulsed-type RF irradiation elicits thermal and/or non-thermal effects by delivering a particular rate of gated RF oscillations. 7,9 With these characteristics, invasive pulsed-type, bipolar AC RF treatment has been deemed effective for use in treating refractory melasma. 10 The skin exhibits various current densities among its multiple layers and also among different types of appendage structures.…”

Section: Discussionmentioning

confidence: 99%

Invasive Pulsed-Type, Bipolar, Alternating Current Radiofrequency Treatment Using Microneedle Electrodes for Nasal Rosacea

Ahn

Cho

2017

Medical Lasers

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Radiofrequency (RF) devices deliver high frequency energy to the biological tissues thath as been either cut or coagulated. A pulsed-type RF irradiation elicits thermal and/or non-thermal effects by delivering a particular rate of gated RF oscillations. In the present study, we describe two patients with nasal rosacea, who were effectively treated with invasive pulsed-type, bipolar alternating current (AC) RF treatment using microneedle electrodes. The RF treatment was delivered to these two patients with a power level of 3, a microneedle penetration depth of 1.5 mm, and 2-3 passes over six (patient 1) and 12 (patient 2) sessions, at two-week intervals. The first patient presented marked clinical improvement with respect to both the overall redness and papulopustular lesions; the second patient exhibited noticeable improvement in both the texture and telangiectatic lesions on the nose. Both patients were satisfied with the clinical outcomes without remarkable side effects. Key words

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“…Electroporation is a technique which allows inducing transient or permanent increased permeability to the cell membrane using pulsed electric fields (PEF) [1]- [3]. Capability to control the permeability of the membrane to various macromolecules allowed to develop a vast array of biotechnological and biomedical methods, such as drug delivery, electrochemotherapy, gene delivery, and many others [3]- [6].…”

Section: Introductionmentioning

confidence: 99%

“…Capability to control the permeability of the membrane to various macromolecules allowed to develop a vast array of biotechnological and biomedical methods, such as drug delivery, electrochemotherapy, gene delivery, and many others [3]- [6].…”

Section: Introductionmentioning

confidence: 99%

Measurement of Transient Permeability of Sp2/0 Myeloma Cells: Flow Cytometric Study

Novickij

Girkontaitė

Grainys

et al. 2016

Measurement Science Review

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Electroporation is an electric field induced phenomenon occurring when the permeability of the cell membrane is increased due to the excess of critical transmembrane potential. Fluorescent dye assays are frequently used for evaluation of the permeabilization rate, however, the protocols vary, which negatively affects the repeatability of the results. In this work we have designed experiments to investigate the protocols and threshold concentrations of the Propidium Iodide (PI) and YO-PRO-1 (YP) fluorescent dyes for evaluation of mammalian cell permeabilization induced by electroporation. The Sp2/0 mouse myeloma cells were used and the bursts of 100 μs x 8 electrical pulses of 0.8-2 kV/cm were applied. It has been shown that the dye concentration has an influence on the detectable permeabilization, and the concentrations below 30 μM for PI and 1 μM for YP should be avoided for measurement of electropermeabilization efficacy due to unreliable fluorescence signals. Further, based on the experimental data, the permeabilization curve for the Sp2/0 myeloma cells in the 0.8-2 kV/cm range has been presented.

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Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges

Cited by 686 publications

References 176 publications

Imaging the dynamics of individual electropores

Imaging the dynamics of individual electropores

Invasive Pulsed-Type, Bipolar, Alternating Current Radiofrequency Treatment Using Microneedle Electrodes for Nasal Rosacea

Measurement of Transient Permeability of Sp2/0 Myeloma Cells: Flow Cytometric Study

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