Variability of HOMA and QUICKI insulin sensitivity indices

Abstract

Assessment of insulin sensitivity based on a single measurement of insulin and glucose, is both easy to understand and simple to perform. The tests most often used are HOMA and QUICKI. The aim of this study was to assess the biological variability of estimates of insulin sensitivity using HOMA and QUICKI indices. After a 12-h fast, blood was sampled for insulin and glucose determination. Sampling lasted for 90 min with an intersample interval of 2 min. A total of 56 subjects were included in the study, and in nine subjects sampling was done before and after weight reduction, so total number of analyzed series was 65. To compute the reference value of the insulin sensitivity index, averages of all 46 insulin and glucose samples were used. We also computed point estimates (single value estimates) of the insulin sensitivity index based on the different number of insulin/glucose samples (1-45 consecutive samples). To compute the variability of point estimates a bootstrapping procedure was used using 1000 resamples for each series and for each number of samples used to average insulin and glucose. Using a single insulin/glucose sample HOMA variability was 26.18 ± 4.31%, and QUICKI variability was 3.30 ± 0.54%. For 10 samples variability was 11.99 ± 2.22% and 1.62 ± 0.31% respectively. Biological variability of insulin sensitivity indices is significant, and it can be reduced by increasing the number of samples. Oscillations of insulin concentration in plasma are the major cause of variability of insulin sensitivity indices.

Sex-Specific Elevated Fasting Glucose in Patients with Major Depressive Disorder

“…Also, the development of prediabetes from the time of enrollment in the study and the 2-year study period positively correlated with the risk of developing MDD [38]. Fasting glucose levels and insulin sensitivity are also closely related [39][40][41]. Interestingly, our study showed no differences in glucose levels and indices for insulin sensitivity between the MDD and non-MDD groups.…”

Аллостазис Глюкозы У Молодых Женщин С Различным Отношением К Употреблению Алкоголя

“…Таким образом, среди ЖУАвТС даже в условиях голодания и угрозы возникновения гипогликемии при УР имеет место более высокая в 2,11 раза (р<0,001) ФАβКл по сравнению с таковой у Тр; не широкое, но значимое распределение гиперинсулинемии среди выпивающих, что указывает на нарушение основного эндокринного механизма регуляции гликемии за счёт снижения чувствительности клеток и тканей к ИРИ и риск развития инсулинорезистентности. Расчёт соответствующих индексов [22,23] инсулиночувствительности («CARO») и инсулинорезистентности («HOMA-Ir») это подтвердил. Так, средние значения обоих индексов в обеих группах респонденток соответствовали норме для здоровых людей, но показывали меньшую чувствительность и большую резистентность к ИРИ клетокмишеней у ЖУАвТС.…”

Optimal Anthropometric Indicators and Cut Points for Predicting Metabolic Syndrome in Chinese Patients with Type 2 Diabetes Mellitus by Gender

“…The alternative methods to HEC, such as the Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), require the measurement of insulin that is not easily accessible and unstable and cannot be applied in areas with poor medical conditions or where large-scale epidemiological screening is required. 3 , 4 …”