Variability and Genetic Structure in Populations of Arrabidaea bilabiata (Sprague) Sandwith in the Amazonas State

Abstract

-Weeds in pastures can intoxicate animals, and Arrabidaea bilabiata is the most important species for herbivores in floodplain areas in the Amazon Basin. Genetic diversity studies in natural populations may contribute to the better understanding of the range of toxicity and the genetic variability organization in this species. The objective of this study was to assess the variability and genetic structure in six populations of A. bilabiata sampled in floodplain areas in three municipalities of the Amazonas State, from the AFLP markers analysis. AFLP markers were efficient to characterize the genetic variability of the 65 individuals analyzed. From four combinations of oligonucleotides, a total of 309 AFLP fragments was obtained, where 304 (98.38%) were polymorphic. By the dendrogram and Bayesian cluster analysis, there was a formation of two isolated groups, the first one comprising individuals from Autazes municipality and the second one comprising individuals from Itacoatiara and Parintins. However, depending on the method to define the most probable cluster number, there was a separation of the six populations, according to their geographical origin. Mantel test confirmed that geographically closer populations are more akin, although low gene flow (0.538) is observed among the sampled populations. The molecular analysis of variance found that 49.29% of the genetic variability are among individuals inside populations and 50.71% among the populations analyzed. The results indicate the possibility that isolated A. bilabiata populations contain plants with different toxicity levels and suggest a strong adaptability of the species.Keywords: Arrabidaea bilabiata, pasture weeds, poisonous plants, AFLP, genetic diversity. RESUMO -Plantas daninhas de pastagens podem causar intoxicações aos animais, sendo a espécie Arrabidaea bilabiata a mais importante para herbívoros nas regiões de várzea da Bacia Amazônica. Estudos de diversidade genética em populações naturais podem contribuir para o melhor entendimento sobre a variação de toxicidade e a organização da variabilidade genética na espécie. O objetivo deste trabalho foi avaliar a variabilidade e estrutura genética em seis populações de

Molecular genetic diversity and mating system in sacha inchi progenies1

“…Since the breeding system is affected by genetic and environmental factors, a genetic variation among the seeds of a plant is the norm, especially in allogamous species. In our study, a considerable genetic diversity was observed within the families (especially 1 and 21), and estimated values are comparable to those found in small populations of another forest climber: Arrabidaea bilabiata (Souza et al 2015).…”