Variabilidade genética em tomate (Solanum lycopersicon Mill.) por marcadores ISSR

Aguilera, Jorge González; Pessoni, Luiz Alberto; Rodrigues, Gabriel Belfort; Elsayed, Ahmed Youssef Abdelnabi Mohamed; Silva, Derly J. H. da; Barros, Everaldo Gonçalves de

doi:10.5039/agraria.v6i2a998

Cited by 18 publications

(2 citation statements)

References 15 publications

(13 reference statements)

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“…These results contrasted with those of Aguilera et al (2011) who, when using ISSR molecular markers to evaluate the genetic variability of 96 tomato accessions, found a lower level of polymorphism corresponding to 34%, with an average of 5,3 polymorphic bands per primer.…”

Section: Resultscontrasting

confidence: 96%

Study of the genetic diversity of tomato (Solanum spp.) with ISSR markers

2020

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In this study 55 tomato genotypes from different geographical regions were evaluated with ISSR markers (Inter Simple Sequence Repeats). The seven ISSR primers originated 63 amplified bands, of which 90.48% were polymorphic. The cluster analysis based on the Nei-Li similarity coefficient using the average genetic clustering method (UPGMA) revealed the conformation of five clusters at a level of similarity of 72%. The ISSR technique did not discriminate tomato genotypes according to the species or region of provenance. The structure analysis and the dendrogram did not reveal a genetic structure in the population evaluated. The genotypes of the species of S. pimpinellifolium, S. l. cerasiforme, S. lycopersicum and S. peruvianum were found consistently grouped, showing a close genetic relationship among them. A high genetic variation among the individuals within each of the groups formed was suggested by the AMOVA. The ISSR markers were effective in assessing the genetic diversity and structure of populations of tomato genotypes. The high genetic variability found in this study indicates the valuable genetic potential present in tomato germplasm, especially of wild species, which could be used for future breeding programs of the species.

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Section: Resultscontrasting

confidence: 96%

Study of the genetic diversity of tomato (Solanum spp.) with ISSR markers

2020

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“…These authors report that the first group also included the great majority of the genotypes (eight genotypes, about 61.54%), in addition to identifying the formation of groups with only one genotype. Aguilera et al (2011), using the Tocher grouping method in a study on genetic diversity between 96 accessions of tomato and two commercial cultivars, reported the formation of two groups, the first group consisted of 98.97% of the genotypes. The results observed in the present study corroborate those obtained by these authors.…”

Section: Groupmentioning

confidence: 99%

Hierarchical and optimization methods for the characterization of tomato genotypes

Peixoto

Almeida

Rocha

et al. 2019

Rev. bras. eng. agríc. ambient.

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The correct characterization of germplasm banks is fundamental for breeders to succeed in breeding programs. Several studies have sought to obtain genotypes with resistance to pests. However, there is no consensus about which methodology is the most appropriate to characterize a germplasm bank of tomato with different levels of resistance to pests. The objective of this study was to compare methods of multivariate analysis for the evaluation of genetic diversity in tomato genotypes with different levels of resistance to pests. The experiments were conducted at the Vegetable Experimental Station of the Federal University of Uberlândia - Monte Carmelo campus (18º 42’ 43.19” South latitude and 47º 29’ 55.8” West longitude, 873 m altitude), in the period from April 2013 to November 2016. Sixteen genotypes were evaluated from the interspecific cross between LA-716 (S. pennellii) versus pre-commercial line (UFU-057) followed by backcrossing and self-fertilization, along with the pre-commercial line UFU-057 (recurrent parent) Santa Clara and the wild accession S. pennellii (donor genitor). The contents of acylsugar, foliar trichomes, South American tomato pinworm and leaf miner repellency tests were analyzed. The experimental design was the randomized block design totaling 76 plots (19 genotypes x 4 blocks). It was concluded that there was genetic variability among the evaluated genotypes. The method of graphic dispersion by principal components revealed a greater power of discrimination. Genotypes UFU-057F2RC27#4.3, UFU-057F2RC28#2.2 and UFU-057F2RC27#4.7 contain the highest levels of acylsugar, resistance to Liriomyza spp. and T. absoluta.

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