Variabilidade genética de populações naturais de caroá por meio de marcadores RAPD

Abstract

Resumo -O objetivo deste trabalho foi quantificar a variabilidade genética entre e dentro de populações de caroá (Neoglaziovia variegata), por meio de marcadores "random amplified polymorphic DNA" (RAPD). Foram analisados 180 genótipos de caroá, provenientes dos municípios de Guanambi, Juazeiro e Valente, no Estado da Bahia. Foi observado elevado polimorfismo entre as populações de caroá. As dissimilaridades genéticas entre os genótipos variaram de 0,08 a 0,95, com média de 0,44. A variância molecular mostrou que 56% da variação total foi explicada pelas diferenças entre indivíduos dentro de locais. As diferenças entre municípios explicaram 17% da variação total, enquanto as diferenças entre locais dentro dos municípios explicaram 26% da variação.Termos para indexação: Neoglaziovia variegata, Bromeliaceae, divergência genética, marcadores moleculares. Genetic variability estimated among caroá populations through RAPD markersAbstract -The objective of this work was to quantify the genetic variability within and among populations of caroá (Neoglaziovia variegata) using random amplified polymorphic DNA (RAPD) markers. One hundred eighty caroá genotypes from Guanambi, Juazeiro and Valente counties in the state of Bahia, Brazil, were analyzed. A high polymorphism was observed among the caroá populations. The genetic dissimilarities among all genotypes ranged from 0.08 to 0.95 with an average of 0.44. The molecular variance showed that 56% of the total variation was explained by the differences among individuals within locations. The differences among counties explained 17% of the total variation, while the differences among places within counties explained 26% of the variation.

Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá

“…Isto se deve ao fato de que suas fibras já foram muito utilizadas nas indústrias têxteis na primeira metade do século passado e recentemente voltaram a ser uma das principais fontes de emprego e renda para diversas famílias nordestinas com a fabricação de produtos artesanais (SILVEIRA et al, 2009a). Contudo, a coleta das plantas para a extração das fibras sempre ocorreu de forma extrativista colocando a sobrevivência da espécie em condição de ameaçada em algumas regiões nordestinas.…”

Comparison of eight methods of genomic DNA extraction from babassu

“…The development of genetic conservation strategies for a species includes the study of the distribution of genetic variability within and among its populations (Lacerda and Kageyama, 2003). Advances in molecular biology have provided several new methods for the genetic studies of natural populations (Silveira et al, 2009).…”

Somatic embryogenesis of Neoglaziovia variegata (Arruda) Mez, an important source of fiber from native Brazilian bromeliads

“…Its fibers are used in the textile industry and fine craftsmanship, constituting a primary source of employment and income for many families in the Northeast (Ribeiro 2007;Silveira et al 2009a). Furthermore, like other plants of the bromeliad family and due to the quality of the fiber, it can be used in the formulation of polymeric compounds for the development of many industrial products (Leão et al 2009;Sena Neto et al 2013).…”

“…In addition, the increased devastation of the Caatinga for the development of agricultural activities has placed this species under endangered conditions. Therefore, there is a need of immediate studies to establish the strategies for genetic conservation and for the development of a cultivation system for this species (Silveira et al 2009a). For both objectives, the basic requirement is the large-scale production of seedlings, associated with studies on its genetic variability as reported in previous studies (Silveira et al 2009b).…”

“…For both objectives, the basic requirement is the large-scale production of seedlings, associated with studies on its genetic variability as reported in previous studies (Silveira et al 2009b). As regards the conservation, a micropropagation protocol for in vitro germination of caroá plants has already been developed along with the results of the genetic variability studies of three distinct caroá populations (Silveira et al 2009a). Nevertheless, in order to grow this plant for use at an industrial level, such as in the case of reinforcement polymeric matrices, or even for fiber production to be used in craftsmanship, a very high number of seedlings in uniform conditions is necessary.…”