Variabilidade genética de isolados de Fusarium spp. e estudo da interação com a planta hospedeira.

Martins, Mayra Kassawara

doi:10.11606/t.11.2005.tde-06062005-153027

Cited by 11 publications

(4 citation statements)

References 119 publications

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“…The RFLP-ITS technique implies the use of only a single region of the genome and cannot be used to distinguish between pathogenic and clinical isolates of F. verticillioides. Martins (2005) could not distinguish phytopathogenic from non-pathogenic isolates of Fusarium species that were obtained from different host plants using the same technique.…”

Section: Discussionmentioning

confidence: 94%

Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans

Chang¹,

Macêdo²,

Souza-Motta³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Fusarium verticillioides is a pathogen of agriculturally important crops, especially maize. It is considered one of the most important pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and animals. Moreover, it is recognized as a cause of localized infections in immunocompetent patients and disseminated infections among severely immunosuppressed patients. Several molecular tools have been used to analyze the intraspecific variability of fungi. The objective of this study was to use molecular markers to compare pathogenic isolates of F. verticillioides and isolates of the same species obtained from clinical samples of patients with Fusarium mycoses. The molecular markers that we used were intersimple sequence repeat markers (primers GTG 5 and GACA 4 ), intron splice site primer (primer EI1), random amplified polymorphic DNA marker (primer OPW-6), and restriction fragment length polymorphism-internal transcribed spacer (ITS) from rDNA. From the data obtained, clusters were generated based on the UPGMA clustering method. The amplification products obtained using primers ITS4 and ITS5 and loci ITS1-5.8-ITS2 of the rDNA yielded fragments of approximately 600 bp for all the isolates. Digestion of the ITS region fragment using restriction enzymes such as EcoRI, DraI, BshI, AluI, HaeIII, HinfI, MspI, and PstI did not permit differentiation among pathogenic and clinical isolates. The inter-simple sequence repeat, intron splice site primer, and random amplified polymorphic DNA markers presented high genetic homogeneity among clinical isolates in contrast to the high variability found among the phytopathogenic isolates of F. verticillioides.

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Section: Discussionmentioning

confidence: 94%

Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans

Chang¹,

Macêdo²,

Souza-Motta³

et al. 2013

Genet. Mol. Res.

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“…A partir da amplificação desses primers, obtiveram-se 178 produtos de amplificação (bandas) (Tabela 2), sendo que 167 (93,82%) apresentaram polimorfismo entre, pelo menos, dois isolados e apenas 11 (6,18%) apresentaram monomorfismo, demonstrando alta variabilidade entre os isolados (Figura 1). Esses valores foram similares aos obtidos por Martins (2005) que, ao realizar análise de polimorfismo em DNA de Fusarium spp., utilizando 14 primers RAPD, observou um total de 166 bandas, sendo 6,1% monomórficas e 93,9% polimórficas. Nozaki (2003) não obteve nenhuma banda monomórfica e 72 bandas polimórficas ao analisar os produtos de amplificação de 7 primers RAPD em diferentes isolados de Fusarium sp.. Silva (2009), trabalhando com 66 isolados de F. oxysporum f. sp.…”

Section: Resultsunclassified

“…A maior distância (54,6%) ocorreu entre o isolado 90 proveniente do município de Nova Porteirinha e 128 provenientes de Janaúba, e a menor (5,7%) entre os isolados 5 e 106 provenientes de Matias Cardoso e Janaúba, respectivamente. Martins (2005) De posse das distâncias genéticas, foi realizada uma análise de agrupamento pelo Método da Ligação Média entre Grupo (UPGMA), com a obtenção do dendrograma (Figura 2). A partir da distância média relativa (55,3%), determinou-se um ponto de corte, que permitiu a formação de 13 grupos.…”

Section: Resultsunclassified

Variabilidade genética de isolados de Fusarium oxysporum f. sp. cubense obtidos de bananais do norte de Minas Gerais

Santos¹,

Costa

Xavier

et al. 2011

Rev. Bras. Frutic.

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RESUMO-A banana é uma das frutas tropicais mais consumidas no mundo, respondendo por, aproximadamente, 10% do comércio mundial de frutas. O mal-do-panamá, causado por Fusarium oxysporum f. sp. cubense (E.F. Smith), é uma das principais doenças da bananeira. Os marcadores moleculares RAPD têm sido extremamente úteis para estudos de identificação taxonômica, análise de variabilidade da virulência em fungos fitopatogênicos, caracterização de raças e variabilidade inter e intraespecifica de populações de diferentes regiões. O objetivo do trabalho foi avaliar a variabilidade genética de 36 culturas monospóricas de isolados de F. oxysporum f. sp. cubense por meio de marcadores moleculares do tipo RAPD. Foram selecionados 13 primers RAPD, e a análise de dados foi realizada utilizando-se do coeficiente de similaridade de Nei e Li. A partir da amplificação dos primers, foram obtidas 178 bandas, sendo que 167 (93,82%) apresentaram polimorfismo entre, pelo menos, dois isolados e apenas 11 (6,18%) apresentaram monomorfismo, demonstrando alta variabilidade entre os isolados. As distâncias genéticas variaram de 5,7 a 54,6%, sendo a distância média de 30,2%, e a distância média relativa, de 55,3%. De acordo com a análise de agrupamento (UPGMA), não foram observadas correlações entre os isolados estudados. Os resultados sugerem alta variabilidade genética entre os isolados avaliados, não ocorrendo agrupamento de acordo com a origem geográfica de coleta. Termos para Indexação: mal-do-panamá, marcadores RAPD, Musa spp., bananeira. GENETIC VARIABILITY OF Fusarium oxysporum f. sp. cubense ISOLATES OBTAINED FROM BANANA ORCHARDS OF THE NORTH OF MINAS GERAISABSTRACT -Banana is one of the most consumed tropical fruits in the world, accounting for approximately 10% of world trade in fruits. Panama disease, caused by Fusarium oxysporum f. sp. cubense is a major disease of banana. RAPD marker have been used for taxonomic studies, analysis of variability of virulence in pathogenic fungi, characterization of races and variability inter and intra-specific populations from different regions. The objective of present study was to evaluate the genetic variability of 36 isolates of F. oxysporum f. sp. cubense using RAPD marker. Thirteen RAPD primers were selected and data analysis was performed using the similarity coefficient of Nei and Li. A total of 178 bands were obtained, of which 167 (93.82%) showed polymorphism at least two isolates and only 11 (6.18%) showed monomorphism, demonstrating the high variability among isolates. The genetic distances ranged from 5.7 to 54.6%, and the average distance of 30.2%. According to cluster analysis (UPGMA) no correlation among isolates were found. The results suggest a high genetic variability among isolates and no correlation between molecular groupings and their geographical origin were observed.

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“…are also associated with losses in vegetables and other crops [8]. The Fusarium genus is mainly composed of soil fungi, with a cosmopolitan distribution and active in the decomposition of plant cell substrates, some of which cause plant diseases [9], such as the species F. graminearum, F. moniliforme, F. oxysporum and F. verticillioides, known to infect cereal, fruit, vegetable crops, among others [10]. Sclerotium rolfsii is a soil polyphagous phytopathogenic fungus that causes significant crop yield losses worldwide, particularly in tropical and subtropical countries [11].…”

Section: Introductionmentioning

confidence: 99%

Identification of Coumarins and Antimicrobial Potential of Ethanolic Extracts of Dipteryx odorata and Dipteryx punctata

et al. 2022

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Dipteryx odorata and Dipteryx punctata are species native to the Amazonian, traded by extractivists to obtain coumarin. We aimed to analyze the presence of coumarin in the ethanolic extracts of leaves, branches and fruits of D. odorata and D. punctata and to evaluate the antimicrobial activity of these extracts against phytopathogenic fungi and bacteria of clinical interest. Chemical analyses were performed by thin layer chromatography (TLC) and by gas chromatography coupled to mass spectrometry (GC-MS). For the antifungal assays, the fungi used were Cercospora longissima, Colletotrichum gloeosporioides, two isolates of Fusarium spp. and Sclerotium rolfsii, and the antibacterial assay was performed using the minimum inhibitory concentration (MIC) test with Burkholderia cepacia, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus bacteria. In D. odorata seed extracts and in D. punctata husks, endocarps, and seeds, we identified 1,2-benzopyrone. D. odorata endocarp extracts and D. punctata seeds provided the greatest decrease in mycelial growth of the evaluated phytopathogens, showing promise as an alternative control. The husk and endocarp extracts of both species had a weak effect on E. coli. This research is the first to compare the different parts of species of the genus Dipteryx and to evaluate the use of husks and endocarps of D. punctata fruits to obtain coumarin. Chemical analyses used to quantify the compounds existing in the extracts, and tests with phytopathogens in vitro and in vivo are currently being carried out.

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Variabilidade genética de isolados de Fusarium spp. e estudo da interação com a planta hospedeira.

Cited by 11 publications

References 119 publications

Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans

Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans

Variabilidade genética de isolados de Fusarium oxysporum f. sp. cubense obtidos de bananais do norte de Minas Gerais

Identification of Coumarins and Antimicrobial Potential of Ethanolic Extracts of Dipteryx odorata and Dipteryx punctata

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