Variabilidad genética de Aedes aegypti en el departamento de Sucre, Colombia, mediante el análisis de la secuencia de nucleótidos del gen mitocondrial ND4

Abstract

Introduction. Aedes aegypti is the most important mosquito species in America for the transmission of viruses of dengue, Zika, Chikungunya and yellow fever. Ecological factors as well as chemical controls can affect the genetic composition of Ae. aegypti populations, which is why its genetic characterization is necessary. Objective. To determine the genetic variability of Ae. aegypti populations in four municipalities of Sucre department, Colombia. Materials and methods. Larvae of Ae. aegypti, collected in the municipalities of Sincelejo, Sampués, Corozal and Guaranda, Sucre department, were reared under laboratory conditions to adult stage. A segment of the mitochondrial ND4 gene which codes for the subunit 4 of the enzyme NADH-dehydrogenase was used as genetic marker. The genetic analysis included the estimation of parameters of nucleotide and haplotype diversity, genetic structure and gene flow. Results. One hundred and eight partial sequences of 357 nucleotides and four nucleotide haplotypes of the ND4 gene of Ae. aegypti were obtained. A significantly high genetic differentiation was found between the Sampués and Guaranda populations (FST=0.59467), Sincelejo and Sampués (FST=0.25637), and Corozal and Guaranda (FST=0.22237). A high gene flow (Nm=infinite) was observed among the populations of Sincelejo and Corozal. Conclusion. There are genetic differences between the Ae. aegypti populations from the municipalities of Sucre department. The presence of a new haplotype of the mitochondrial ND4 gene of Ae. aegypti in Colombia was recorded, detected in the municipality of Sincelejo.

Population structure and ancestry prediction of Aedes aegypti (Diptera: Culicidae) supports a single African origin of Colombian populations

“…Microgeographic structure of Colombian populations has been slightly traced so far, but no clear picture of population demographic has been supported. Several studies at local level using dominant genetic markers such as RAPDs, (14) and nucleotide sequence analyses of mitochondrial genes, such as COI (17) and NADH dehydrogenase subunit 4 (ND4) (15,16) unveiled Colombian Ae. aegypti populations show moderated population differences at spatiotemporal scales, and it was suggested as a consequence of local pressures.…”

“…(11,12,13) In Colombia, a first study based on random amplified polymorphic DNA-polymerase chain reaction products analysis (RAPD) reported local genetic population substructure in city of Cali, which was suggested involved in differences of vector competence and insecticide resistance ratios. (14) Likewise, genetic analyses based on partial nucleotide sequences of mitochondrial NADH dehydrogenase 4 (ND4) gene, disclosed local microgeographic differences among individuals of municipalities of Sincelejo, Guaranda, Corozal and Sampués (within a maximum range of ~100 km) from Sucre department, (15,16) as well as from Medellin city. (17) These studies would suggest a complex heterogeneous population substructure is placed throughout Colombian populations mainly as consequence of local differences on selective pressures from the chemical control.…”

Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

“…The A. aegypti and A. albopictus classification of individuals is mainly determined by morphological identification (Kraemer et al, 2015), and few reports use molecular identification (Zamora-Delgado et al, 2015;Atencia et al, 2018). Although, morphological identification is the most widely used classification technique, it has different limitations.…”

Genetic variation ofAedes aegyptipopulations from Ecuador

“…Several studies have shown the genetic variation of Ae. aegypti populations in the neighboring countries, Perú (Costa-da-Silva et al 2005), Colombia (Jaimes-Dueñez et al 2015, Atencia et al 2018) and Brazil (Monteiro et al 2014, Paduan and Ribolla 2008). In Ecuador, there were two haplotypes, which show low genetic diversity in all the sampled localities, although the ecological differences among the geographical areas are apparent among the Pacific coast, Amazon basin and the Galápagos Islands.…”