Vantagens e desvantagens das colunas C18 e C30 para a separação de carotenóides por CLAE

Abstract

INTRODUÇÃONos últimos anos, o licopeno vem ganhando destaque devido ao seu elevado potencial antioxidante e associação ao menor risco de desenvolvimento de cân-cer de próstata (Van den Berg et al., 2000;Giovannucci, 2002;Venkateswaran et al., 2004). Estudos têm demonstrado que o elevado consumo de luteína e zeaxantina está relacionado à significativa redução da catarata e da degeneração macular (Junghans et al., 2001;Stringham, Hammond, 2005).Colunas de fase reversa C 18 e C 30 vêm sendo amplamente utilizadas para separação de carotenóides. Em geral, melhores separações de carotenos e isômeros têm

Encapsulation of lycopene using spray-drying and molecular inclusion processes

“…The chromatograms were processed at 470 nm and the spectra were obtained between 250 and 600 nm. Separation of lycopene from spray-dried microcapsules was achieved on a polymeric YMC C 30 column (3 µm, 250 x 4.6 mm) with MeOH (0.1% triethylamine (TEA))/t-butyl methyl ether (TBME) (1:1 v/v) at 1 mL/min and column temperature set at 33 o C, whereas separation of lycopene from the β-CD complex was carried out on a monomeric C 18 Novapack (4 µm, 300 x 3.9 mm) column using as mobile phase a linear gradient of MeOH (0.1% TEA)/ethyl acetate (EtOAc)/H 2 O from 8:1:1 to 7:3:0 (v/v) in 20 min at 1 mL/min and temperature set at 29 o C. Both conditions were previously evaluated by Nunes and Mercadante (2006). The lycopene isomers were identified, in both the columns, by comparison of the spectra features and elution order with data from literature (Hengartner et al;1992;Mercadante et al, 1999;Nunes and Mercadante, 2004) and with authentic standards of all-trans-, 5-cis-, 9-cis-and 13-cis-lycopene from DSM Nutritional Products (Switzerland).…”

A Comprehensive Quality Evaluation Method Based on C30-HPLC and an Analytic Hierarchy Process for the Chinese Herbal Formula, Erzhiwan

“…It was also widely used in improving the method for separating retinol [ 22 ], all-trans lycopene, and β -carotene [ 23 ]. Therefore, separation with a C 30 column was more effective than that with a C 18 column for non-polar geometric isomers [ 24 ].…”